UBF1 Antibody - #AF7743

(2)  
Product: UBF1 Antibody
Catalog: AF7743
Description: Rabbit polyclonal antibody to UBF1
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90KD; 89kD(Calculated).
Uniprot: P17480
RRID: AB_2844107

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
UBF1 Antibody detects endogenous levels of total UBF1.
RRID:
AB_2844107
Cite Format: Affinity Biosciences Cat# AF7743, RRID:AB_2844107.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;

Immunogens

Immunogen:
Uniprot:

P17480(UBF1_HUMAN) >>Visit HPA database.

Gene(ID):
UBTF(7343)
Sequence:
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17480 As Substrate

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
T9 Phosphorylation
K17 Ubiquitination
S23 Phosphorylation
K35 Ubiquitination
S44 Phosphorylation
K45 Ubiquitination
K47 Ubiquitination
K57 Ubiquitination
K61 Acetylation
K61 Ubiquitination
K69 Ubiquitination
K71 Ubiquitination
K97 Ubiquitination
K107 Ubiquitination
K114 Acetylation
K114 Sumoylation
K114 Ubiquitination
T117 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
K126 Ubiquitination
K132 Ubiquitination
K144 Ubiquitination
S147 Phosphorylation
Y150 Phosphorylation
K151 Ubiquitination
K160 Sumoylation
K160 Ubiquitination
K169 Ubiquitination
K191 Ubiquitination
K198 Ubiquitination
K200 Ubiquitination
T201 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
Y207 Phosphorylation
T208 Phosphorylation
K211 Ubiquitination
K216 Ubiquitination
K224 Ubiquitination
K232 Methylation
K232 Ubiquitination
S238 Phosphorylation
K240 Ubiquitination
K244 Ubiquitination
K248 Ubiquitination
K254 Methylation
K254 Ubiquitination
K266 Ubiquitination
S273 Phosphorylation
K279 Ubiquitination
K284 Acetylation
K284 Sumoylation
K284 Ubiquitination
S306 Phosphorylation
Y308 Phosphorylation
S329 Phosphorylation
K333 Ubiquitination
S336 Phosphorylation
K338 Ubiquitination
K352 Acetylation
K352 Ubiquitination
S364 Phosphorylation
K378 Ubiquitination
K384 Ubiquitination
K385 Acetylation
T388 Phosphorylation
S389 Phosphorylation P24941 (CDK2)
S392 Phosphorylation
K394 Acetylation
K401 Acetylation
K406 Acetylation
S412 Phosphorylation
K421 Ubiquitination
R429 Methylation
S433 Phosphorylation
S435 Phosphorylation
R443 Methylation
S449 Phosphorylation
Y456 Phosphorylation
K464 Sumoylation
K480 Ubiquitination
S484 Phosphorylation P24941 (CDK2) , P11802 (CDK4)
S495 Phosphorylation
Y500 Phosphorylation
S546 Phosphorylation
K559 Acetylation
S584 Phosphorylation
K594 Ubiquitination
S602 Phosphorylation
S610 Phosphorylation
K624 Ubiquitination
S638 Phosphorylation
R642 Methylation
K646 Ubiquitination
Y648 Phosphorylation
S655 Phosphorylation
S666 Phosphorylation

Research Backgrounds

Function:

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

PTMs:

Phosphorylated and activated by PIK3CA.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.

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For Research Use Only.
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