SF1 Antibody | Affinity Biosciences

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Product:	SF1 Antibody
Catalog:	AF7895
Description:	Rabbit polyclonal antibody to SF1
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.:	68kDa; 68kD(Calculated).
Uniprot:	Q15637
RRID:	AB_2844259

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SF1 Antibody detects endogenous levels of total SF1.

RRID:

AB_2844259
Cite Format: Affinity Biosciences Cat# AF7895, RRID:AB_2844259.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BBP; D11S636; Mammalian branch point binding protein mBBP; OTTHUMP00000069749; OTTHUMP00000069750; OTTHUMP00000069752; Splicing factor 1; Transcription factor ZFM1; ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZNF162; SF1;

Immunogens

Immunogen:

Uniprot:

Q15637(SF01_HUMAN) >>Visit HPA database.

Gene(ID):

SF1(7536)

Expression:

Q15637 SF01_HUMAN:

Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

Sequence:

Q15637 SF01_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Rabbit

100

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15637 As Substrate

Q15637

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T8	Phosphorylation		Uniprot
S14	Phosphorylation		Uniprot
S20	Phosphorylation		Uniprot
T26	Phosphorylation		Uniprot
T67	Phosphorylation		Uniprot
S80	Phosphorylation	Q8TAS1 (UHMK1)	Uniprot
S82	Phosphorylation	Q8TAS1 (UHMK1)	Uniprot
Y87	Phosphorylation		Uniprot
S89	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
K217	Acetylation		Uniprot
K217	Ubiquitination		Uniprot
K227	Ubiquitination		Uniprot
T232	Phosphorylation		Uniprot
T253	Phosphorylation		Uniprot
R263	Methylation		Uniprot
S267	Phosphorylation		Uniprot
S268	Phosphorylation		Uniprot
S272	Phosphorylation		Uniprot
T274	Phosphorylation		Uniprot
T277	Phosphorylation		Uniprot
C279	S-Nitrosylation		Uniprot
S302	Phosphorylation		Uniprot
K306	Acetylation		Uniprot
K306	Ubiquitination		Uniprot
Y313	Phosphorylation		Uniprot
T338	Phosphorylation		Uniprot
R345	Methylation		Uniprot
R365	Methylation		Uniprot

Research Backgrounds

Q15637_SF01_HUMAN

Function:

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

PTMs:

Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.

Subcellular Location:

Nucleus.

Tissue Specificity:

Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

Subunit Structure:

Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Interacts with RBM17.

Family&Domains:

Belongs to the BBP/SF1 family.

References

1). Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis. Frontiers in Marine Science, 2020 [IF=3.7]

Application: WB Species: Human Sample: ovarian tissue

FIGURE 7. Protein expression of Aromatase and AC/cAMP/PKA/CREB/SF-1 pathway in ovarian tissue of E. sinensis subjected to different levels of dietary ICA. The relative expression levels of aromatase were normalized to that of β-actin. Values represent means ± SEM (n = 3). Bars assigned different superscripts are significantly different

2). BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells. Theriogenology, 2023 (PubMed: 36525856) [IF=2.4]

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For Research Use Only.
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SF1 Antibody - #AF7895