PASK Antibody - #DF10342

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Product: PASK Antibody
Catalog: DF10342
Description: Rabbit polyclonal antibody to PASK
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 142KD; 143kD(Calculated).
Uniprot: Q96RG2
RRID: AB_2844349

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PASK Antibody detects endogenous levels of total PASK.
RRID:
AB_2844349
Cite Format: Affinity Biosciences Cat# DF10342, RRID:AB_2844349.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZP434O051; DKFZp686P2031; hPASK; KIAA0135; MGC93882; mKIAA0135; PAS domain containing serine/threonine kinase; PAS domain containing serine/threonine protein kinase; PAS domain-containing serine/threonine-protein kinase; PAS kinase; PAS serine/threonine kinase; PAS-kinase; Pask; PASK_HUMAN; PASKIN; STK 37; STK37;

Immunogens

Immunogen:
Uniprot:

Q96RG2(PASK_HUMAN) >>Visit HPA database.

Gene(ID):
PASK(23178)
Expression:
Q96RG2 PASK_HUMAN:

Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).

Sequence:
MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAKTTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEKIPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITDLIPSVQLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLIPGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIRKLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESPGQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQLSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RG2 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S19 Phosphorylation
S40 Phosphorylation
S42 Phosphorylation
S55 Phosphorylation
S60 Phosphorylation
S104 Phosphorylation
S106 Phosphorylation
S109 Phosphorylation
S116 Phosphorylation
K132 Ubiquitination
K218 Acetylation
T307 Phosphorylation
K314 Ubiquitination
K316 Ubiquitination
S333 Phosphorylation
Y389 Phosphorylation
S390 Phosphorylation
S524 Phosphorylation
S533 Phosphorylation
K570 Ubiquitination
S579 Phosphorylation
S582 Phosphorylation
K594 Ubiquitination
S667 Phosphorylation
S670 Phosphorylation
S843 Phosphorylation
T874 Phosphorylation
K930 Ubiquitination
S939 Phosphorylation
S949 Phosphorylation
T954 Phosphorylation
K981 Ubiquitination
Y999 Phosphorylation
S1000 Phosphorylation
T1001 Phosphorylation
S1003 Phosphorylation
K1034 Ubiquitination
K1045 Ubiquitination
S1057 Phosphorylation
K1122 Ubiquitination
S1149 Phosphorylation Q96RG2 (PASK)
T1161 Phosphorylation Q96RG2 (PASK)
T1165 Phosphorylation Q96RG2 (PASK)
K1270 Ubiquitination
S1273 Phosphorylation Q96RG2 (PASK)
S1277 Phosphorylation Q96RG2 (PASK)
S1280 Phosphorylation Q96RG2 (PASK)
S1287 Phosphorylation Q96RG2 (PASK)
S1289 Phosphorylation Q96RG2 (PASK)
T1322 Phosphorylation
S1323 Phosphorylation

PTMs - Q96RG2 As Enzyme

Substrate Site Source
P13807 (GYS1) S641 Uniprot
P52945 (PDX1) T151 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P68104 (EEF1A1) T432 Uniprot
Q96RG2 (PASK) S1149 Uniprot
Q96RG2 (PASK) T1161 Uniprot
Q96RG2 (PASK) T1165 Uniprot
Q96RG2 (PASK) S1273 Uniprot
Q96RG2 (PASK) S1277 Uniprot
Q96RG2 (PASK) S1280 Uniprot
Q96RG2 (PASK) S1287 Uniprot
Q96RG2 (PASK) S1289 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.

PTMs:

Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).

Family&Domains:

The protein kinase domain mediates binding to phosphatidylinositol.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

References

1). Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis. Journal of agricultural and food chemistry, 2023 (PubMed: 37943949) [IF=5.7]

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