Eg5 Antibody - #AF4745

(1)   (3)  
Product: Eg5 Antibody
Catalog: AF4745
Description: Rabbit polyclonal antibody to Eg5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 120 kDa; 119kD(Calculated).
Uniprot: P52732
RRID: AB_2844603

View similar products>>

   Size Price Inventory
 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(83%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
Eg5 Antibody detects endogenous levels of total Eg5.
RRID:
AB_2844603
Cite Format: Affinity Biosciences Cat# AF4745, RRID:AB_2844603.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EG5; HKSP; KIF11; KIF11_HUMAN; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5;

Immunogens

Immunogen:
Uniprot:

P52732(KIF11_HUMAN) >>Visit HPA database.

Gene(ID):
KIF11(3832)
Sequence:
MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVKEEYITSALESTEEKLHDAASKLLNTVEETTKDVSGLHSKLDRKKAVDQHNAEAQDIFGKNLNSLFNNMEELIKDGSSKQKAMLEVHKTLFGNLLSSSVSALDTITTVALGSLTSIPENVSTHVSQIFNMILKEQSLAAESKTVLQELINVLKTDLLSSLEMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Rabbit
92
Pig
83
Dog
83
Zebrafish
83
Chicken
83
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52732 As Substrate

Site PTM Type Enzyme
K34 Ubiquitination
S36 Phosphorylation
K60 Ubiquitination
S62 Phosphorylation
T114 Phosphorylation
S120 Phosphorylation
Y125 Phosphorylation
T126 Phosphorylation
K146 Acetylation
K197 Ubiquitination
K207 Ubiquitination
Y211 Phosphorylation
K216 Ubiquitination
T223 Phosphorylation
T226 Phosphorylation
Y231 Phosphorylation
S232 Phosphorylation
S233 Phosphorylation
S240 Phosphorylation
K257 Ubiquitination
K260 Ubiquitination
S291 Phosphorylation
T294 Phosphorylation
S323 Phosphorylation
K357 Ubiquitination
K362 Ubiquitination
K372 Acetylation
K376 Acetylation
K376 Ubiquitination
K395 Ubiquitination
Y399 Phosphorylation
S401 Phosphorylation
K444 Ubiquitination
K451 Ubiquitination
K457 Ubiquitination
T458 Phosphorylation
T463 Phosphorylation
T464 Phosphorylation
K466 Ubiquitination
K472 Ubiquitination
K477 Ubiquitination
Y480 Phosphorylation
K491 Ubiquitination
K498 Ubiquitination
T506 Phosphorylation
T507 Phosphorylation
K508 Ubiquitination
K516 Ubiquitination
K521 Ubiquitination
K536 Ubiquitination
S540 Phosphorylation
K550 Ubiquitination
K618 Ubiquitination
T643 Phosphorylation
S646 Phosphorylation
S652 Phosphorylation
K655 Ubiquitination
K659 Ubiquitination
T663 Phosphorylation
K667 Ubiquitination
K712 Ubiquitination
K715 Ubiquitination
K724 Ubiquitination
K739 Ubiquitination
K745 Ubiquitination
K757 Ubiquitination
K764 Ubiquitination
K771 Ubiquitination
R784 Methylation
K792 Ubiquitination
K803 Ubiquitination
K810 Ubiquitination
S852 Phosphorylation
S858 Phosphorylation
K875 Ubiquitination
K890 Ubiquitination
K908 Ubiquitination
K918 Ubiquitination
T923 Phosphorylation
T925 Phosphorylation
T926 Phosphorylation P06493 (CDK1)
K930 Ubiquitination
S931 Phosphorylation
Y932 Phosphorylation
Y934 Phosphorylation
S936 Phosphorylation
K952 Ubiquitination
R1025 Methylation
T1029 Phosphorylation
S1033 Phosphorylation Q9HC98 (NEK6)
K1034 Ubiquitination
K1045 Ubiquitination

Research Backgrounds

Function:

Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.

PTMs:

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).

A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

References

1). Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway. International Journal of Oncology, 2023 (PubMed: 36929198) [IF=4.5]

Application: WB    Species: Human    Sample: NSCLC cells

Figure 6 KIF11, KIF11-CDK1-CDC25C-cyclinB1 oncogenic network and NF-kB/JNK signaling pathway are the potential CVB-D therapeutic targets in the NSCLC cells. (A) Venn diagram showed the overlap number of potential CVB-D target genes in NSCLC cells by intersecting four LUAD datasets. (B) Protein-protein interaction network analysis revealed interaction between the 10 overlapping DEGs. The disconnected nodes are hidden. (C) The interactive heatmap from GEPIA 2 database showed expression of the 6 DEGs in LUAD and normal lung tissues. KIF11 is the most differentially expressed gene between LUAD and normal lung tissues. (D) Venn diagram revealed 2 common genes (KIF11 and CDK1) based on intersection between the 10 overlapping DEGs and the 100 predicted CVB-D target genes extracted from the Swisstarget prediction database. (E) Western blot analysis identified the expression of KIF11, CDK1, CDC25C and cyclinB1 proteins in the control and CVB-D-treated NSCLC cells. (F and G) Immunohistochemical assay results showed the expression levels and localization of the KIF11 protein in the control and CVB-D-treated A549 xenograft tumor tissues. (H) Western blot assay results revealed the KIF11 protein levels in the si-NC and si-KIF11-transfected NSCLC cells. (I) Western blot analysis demonstrated the expression levels of the oncogenic signaling network proteins (KIF11, CDK1, CDC25C and CyclinB1) and the NF-κB/JNK signaling pathway proteins (p65, p-p65, JNK and p-JNK) in the si-NC- and si-KIF11-transfected NSCLC cells. **P

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.