Desmoplakin Antibody - #AF4709

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Product: Desmoplakin Antibody
Catalog: AF4709
Description: Rabbit polyclonal antibody to Desmoplakin
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 250kDa; 332kD(Calculated).
Uniprot: P15924
RRID: AB_2844714

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
Desmoplakin Antibody detects endogenous levels of total Desmoplakin.
RRID:
AB_2844714
Cite Format: Affinity Biosciences Cat# AF4709, RRID:AB_2844714.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin I; Desmoplakin II; DP I; DP II; DPI; DPII; DSP; KPPS2; PPKS2;

Immunogens

Immunogen:
Uniprot:

P15924(DESP_HUMAN) >>Visit HPA database.

Gene(ID):
DSP(1832)
Expression:
P15924 DESP_HUMAN:

Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.

Sequence:
MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSIRKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSNKPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNPLAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDYMKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTVTTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLADQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKLENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLKKIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRIDKQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQKNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPSGVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDANSENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKELNEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKEKEYEIERLRVLLQEEGTRKREYENELAKVRNHYNEEMSNLRNKYETEINITKTTIKEISMQKEDDSKNLRNQLDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQQKACGSEIMQKKQHLEIELKQVMQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEFQEEAKRRWEYENELSKVRNNYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQIDNLTRENRSLSEEIKRLKNTLTQTTENLRRVEEDIQQQKATGSEVSQRKQQLEVELRQVTQMRTEESVRYKQSLDDAAKTIQDKNKEIERLKQLIDKETNDRKCLEDENARLQRVQYDLQKANSSATETINKLKVQEQELTRLRIDYERVSQERTVKDQDITRFQNSLKELQLQKQKVEEELNRLKRTASEDSCKRKKLEEELEGMRRSLKEQAIKITNLTQQLEQASIVKKRSEDDLRQQRDVLDGHLREKQRTQEELRRLSSEVEALRRQLLQEQESVKQAHLRNEHFQKAIEDKSRSLNESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNATILELRSQLQISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKNQCTQVVQERESLLVKIKVLEQDKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRKEEAIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERSRYQREIDKLRQRPYGSHRETQTECEWTVDTSKLVFDGLRKKVTAMQLYECQLIDKTTLDKLLKGKKSVEEVASEIQPFLRGAGSIAGASASPKEKYSLVEAKRKKLISPESTVMLLEAQAATGGIIDPHRNEKLTVDSAIARDLIDFDDRQQIYAAEKAITGFDDPFSGKTVSVSEAIKKNLIDRETGMRLLEAQIASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRDSQKNFVDPVTKKKVSYVQLKERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSGILRPSTVNELESGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRPGTALELLEAQAATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPETGNIISLFQAMNKELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDIAYKRGYFNEELSEILSDPSDDTKGFFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLRKRRVVIVDPETNKEMSVQEAYKKGLIDYETFKELCEQECEWEEITITGSDGSTRVVLVDRKTGSQYDIQDAIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGTSSSMGSGVSDDVFSSSRHESVSKISTISSVRNLTIRSSSFSDTLEESSPIAAIFDTENLEKISITEGIERGIVDSITGQRLLEAQACTGGIIHPTTGQKLSLQDAVSQGVIDQDMATRLKPAQKAFIGFEGVKGKKKMSAAEAVKEKWLPYEAGQRFLEFQYLTGGLVDPEVHGRISTEEAIRKGFIDGRAAQRLQDTSSYAKILTCPKTKLKISYKDAINRSMVEDITGLRLLEAASVSSKGLPSPYNMSSAPGSRSGSRSGSRSGSRSGSRSGSRRGSFDATGNSSYSYSYSFSSSSIGH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15924 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S7 Phosphorylation
T13 Phosphorylation
R19 Methylation
S22 Phosphorylation
R27 Methylation
Y28 Phosphorylation
T31 Phosphorylation
S32 Phosphorylation
T37 Phosphorylation
S38 Phosphorylation
Y41 Phosphorylation
Y42 Phosphorylation
S43 Phosphorylation
R45 Methylation
T49 Phosphorylation
S53 Phosphorylation
Y56 Phosphorylation
C57 S-Nitrosylation
T59 Phosphorylation
T61 Phosphorylation
K94 Ubiquitination
Y95 Phosphorylation
T102 Phosphorylation
K140 Ubiquitination
K154 Ubiquitination
S157 Phosphorylation
S165 Phosphorylation
S166 Phosphorylation
K167 Ubiquitination
Y172 Phosphorylation
T173 Phosphorylation
S176 Phosphorylation
S178 Phosphorylation
S227 Phosphorylation
Y231 Phosphorylation
K245 Ubiquitination
S246 Phosphorylation
Y249 Phosphorylation
Y255 Phosphorylation
S299 Phosphorylation
K301 Acetylation
K308 Acetylation
S313 Phosphorylation
S317 Phosphorylation
K330 Ubiquitination
K393 Ubiquitination
S398 Phosphorylation
K427 Ubiquitination
K441 Ubiquitination
S442 Phosphorylation
K449 Ubiquitination
K459 Ubiquitination
K478 Ubiquitination
K607 Ubiquitination
S610 Phosphorylation
T613 Phosphorylation
K689 Ubiquitination
K710 Ubiquitination
K727 Methylation
K738 Ubiquitination
K803 Ubiquitination
K815 Ubiquitination
K821 Acetylation
K829 Ubiquitination
K834 Ubiquitination
K855 Ubiquitination
K859 Ubiquitination
R866 Methylation
K872 Ubiquitination
K883 Ubiquitination
K900 Ubiquitination
S911 Phosphorylation
S914 Phosphorylation
K916 Ubiquitination
K931 Ubiquitination
K940 Acetylation
K940 Ubiquitination
S944 Phosphorylation
K949 Ubiquitination
S957 Phosphorylation
Y961 Phosphorylation
K1028 Ubiquitination
K1033 Ubiquitination
R1042 Methylation
K1056 Acetylation
K1056 Ubiquitination
K1064 Ubiquitination
Y1065 Phosphorylation
K1073 Methylation
K1073 Ubiquitination
K1075 Ubiquitination
S1078 Phosphorylation
K1083 Ubiquitination
K1099 Ubiquitination
Y1101 Phosphorylation
K1105 Ubiquitination
Y1116 Phosphorylation
K1126 Ubiquitination
S1127 Phosphorylation
K1136 Ubiquitination
Y1139 Phosphorylation
K1144 Ubiquitination
K1157 Ubiquitination
K1167 Acetylation
Y1169 Phosphorylation
T1183 Phosphorylation
K1185 Ubiquitination
Y1199 Phosphorylation
K1209 Ubiquitination
T1220 Phosphorylation
K1222 Ubiquitination
S1225 Phosphorylation
R1241 Methylation
K1250 Ubiquitination
S1259 Phosphorylation
S1282 Phosphorylation
K1310 Ubiquitination
T1319 Phosphorylation
K1350 Ubiquitination
S1361 Phosphorylation
T1373 Phosphorylation
Y1391 Phosphorylation
K1410 Ubiquitination
K1434 Ubiquitination
Y1512 Phosphorylation
K1527 Ubiquitination
K1529 Ubiquitination
Y1542 Phosphorylation
K1552 Ubiquitination
K1570 Ubiquitination
T1583 Phosphorylation
S1585 Phosphorylation
K1593 Acetylation
K1606 Ubiquitination
T1613 Phosphorylation
S1623 Phosphorylation
S1629 Phosphorylation
S1658 Phosphorylation
S1659 Phosphorylation
K1676 Ubiquitination
K1700 Ubiquitination
S1708 Phosphorylation
T1714 Phosphorylation
R1728 Methylation
R1735 Methylation
K1745 Ubiquitination
K1789 Ubiquitination
K1802 Methylation
K1802 Ubiquitination
K1818 Ubiquitination
K1820 Ubiquitination
K1826 Ubiquitination
K1840 Ubiquitination
S1841 Phosphorylation
K1857 Ubiquitination
K1868 Ubiquitination
K1965 Ubiquitination
Y1981 Phosphorylation
S2000 Phosphorylation
S2022 Phosphorylation
S2024 Phosphorylation
Y2029 Phosphorylation
S2030 Phosphorylation
K2066 Ubiquitination
S2071 Phosphorylation
R2083 Methylation
Y2087 Phosphorylation
K2103 Ubiquitination
S2108 Phosphorylation
K2145 Ubiquitination
Y2159 Phosphorylation
S2161 Phosphorylation
K2170 Ubiquitination
S2202 Phosphorylation
S2207 Phosphorylation
S2209 Phosphorylation
T2218 Phosphorylation
S2225 Phosphorylation
S2242 Phosphorylation
Y2264 Phosphorylation
K2271 Ubiquitination
Y2275 Phosphorylation
Y2316 Phosphorylation
K2317 Acetylation
K2317 Ubiquitination
K2328 Ubiquitination
K2361 Ubiquitination
S2383 Phosphorylation
K2393 Ubiquitination
S2406 Phosphorylation
Y2426 Phosphorylation
K2430 Ubiquitination
K2450 Acetylation
T2460 Phosphorylation
T2473 Phosphorylation
S2478 Phosphorylation
K2484 Acetylation
Y2490 Phosphorylation
K2523 Ubiquitination
T2524 Phosphorylation
S2526 Phosphorylation
K2536 Ubiquitination
Y2547 Phosphorylation
S2549 Phosphorylation
S2551 Phosphorylation
S2553 Phosphorylation
T2555 Phosphorylation
S2562 Phosphorylation
S2589 Phosphorylation
K2592 Ubiquitination
S2597 Phosphorylation
S2598 Phosphorylation
T2603 Phosphorylation
S2606 Phosphorylation
S2607 Phosphorylation
S2608 Phosphorylation
S2610 Phosphorylation
T2612 Phosphorylation
S2632 Phosphorylation
K2702 Ubiquitination
S2708 Phosphorylation
Y2720 Phosphorylation
Y2731 Phosphorylation
T2733 Phosphorylation
S2746 Phosphorylation
Y2785 Phosphorylation
K2786 Ubiquitination
S2792 Phosphorylation
T2798 Phosphorylation
S2810 Phosphorylation
K2811 Ubiquitination
S2815 Phosphorylation
Y2817 Phosphorylation
S2820 Phosphorylation
S2821 Phosphorylation
S2825 Phosphorylation
R2826 Methylation
S2827 Phosphorylation
S2829 Phosphorylation
R2830 Methylation
S2831 Phosphorylation
S2833 Phosphorylation
R2834 Methylation
S2837 Phosphorylation
S2839 Phosphorylation

Research Backgrounds

Function:

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

PTMs:

Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.

Subcellular Location:

Cell junction>Desmosome. Cytoplasm>Cytoskeleton. Cell membrane.
Note: Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.

Subunit Structure:

Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Interacts with PKP2. Interacts weakly with TMEM65 (By similarity).

Family&Domains:

Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.

The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.

The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.

Belongs to the plakin or cytolinker family.

Research Fields

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

References

1). SerpinA3N Regulates the Secretory Phenotype of Mouse Senescent Astrocytes Contributing to Neurodegeneration. The journals of gerontology. Series A, Biological sciences and medical sciences, 2024 (PubMed: 38175667) [IF=4.3]
2). Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal. International Urology and Nephrology, 2023 (PubMed: 37594700) [IF=1.8]

Application: WB    Species: Human    Sample:

Fig. 1 Serpina3/serpina3n expression was down-regulated in IC/BPS. A Assessment of the pathological changes in human bladder tissues by HE staining (× 200). B Evaluation of mast-cell infiltration in human bladder tissues using toluidine blue staining (× 200). C, D The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. E Assessment of the pathological changes in mouse bladder tissues by HE staining (× 200). F Evaluation of mast-cell infiltration in mouse bladder tissues using toluidine blue staining (× 200). G, H The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. (*P 

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