Product: IFT88 Antibody
Catalog: DF12001
Description: Rabbit polyclonal antibody to IFT88
Application: WB IHC
Reactivity: Human, Mouse, Rat, Dog
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 88-95 kDa; 94kD(Calculated).
Uniprot: Q13099
RRID: AB_2844806

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Dog
Prediction:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
IFT88 Antibody detects endogenous levels of total IFT88.
RRID:
AB_2844806
Cite Format: Affinity Biosciences Cat# DF12001, RRID:AB_2844806.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D13S1056E; DAF19; hTg737; Ift88; IFT88_HUMAN; Intraflagellar transport 88 homolog; Intraflagellar transport protein 88 homolog; MGC26259; Polaris homolog; Probe hTg737 (polycystic kidney disease, autosomal recessive); Recessive polycystic kidney disease protein Tg737 homolog; RP11-172H24.2; Tetratricopeptide repeat domain 10; Tetratricopeptide repeat protein 10; TG737; TPR repeat protein 10; TTC10;

Immunogens

Immunogen:

A synthesized peptide derived from human IFT88, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q13099 IFT88_HUMAN:

Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Sequence:
MKFTNTKVQMMQNVHLAPETDEDDLYSGYNDYNPIYDIEELENDAAFQQAVRTSHGRRPPITAKISSTAVTRPIATGYGSKTSLASSIGRPMTGAIQDGVTRPMTAVRAAGFTKAALRGSAFDPLSQSRGPASPLEAKKKDSPEEKIKQLEKEVNELVEESCIANSCGDLKLALEKAKDAGRKERVLVRQREQVTTPENINLDLTYSVLFNLASQYSVNEMYAEALNTYQVIVKNKMFSNAGILKMNMGNIYLKQRNYSKAIKFYRMALDQVPSVNKQMRIKIMQNIGVTFIQAGQYSDAINSYEHIMSMAPNLKAGYNLTICYFAIGDREKMKKAFQKLITVPLEIDEDKYISPSDDPHTNLVTEAIKNDHLRQMERERKAMAEKYIMTSAKLIAPVIETSFAAGYDWCVEVVKASQYVELANDLEINKAVTYLRQKDYNQAVEILKVLEKKDSRVKSAAATNLSALYYMGKDFAQASSYADIAVNSDRYNPAALTNKGNTVFANGDYEKAAEFYKEALRNDSSCTEALYNIGLTYEKLNRLDEALDCFLKLHAILRNSAEVLYQIANIYELMENPSQAIEWLMQVVSVIPTDPQVLSKLGELYDREGDKSQAFQYYYESYRYFPCNIEVIEWLGAYYIDTQFWEKAIQYFERASLIQPTQVKWQLMVASCFRRSGNYQKALDTYKDTHRKFPENVECLRFLVRLCTDLGLKDAQEYARKLKRLEKMKEIREQRIKSGRDGSGGSRGKREGSASGDSGQNYSASSKGERLSARLRALPGTNEPYESSSNKEIDASYVDPLGPQIERPKTAAKKRIDEDDFADEELGDDLLPE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
92
Xenopus
92
Rabbit
92
Zebrafish
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cell projection>Cilium>Flagellum.
Note: Colocalizes with ENTR1 and gamma-tubulin at the basal body of primary cilia. Colocalizes with ENTR1 and pericentrin at the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Restrictive clause

 

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For Research Use Only.
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