MFF Antibody - #DF12006

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Product: MFF Antibody
Catalog: DF12006
Description: Rabbit polyclonal antibody to MFF
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 26-29, 35-38 kDa; 38kD(Calculated).
Uniprot: Q9GZY8
RRID: AB_2844811

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
MFF Antibody detects endogenous levels of total MFF.
RRID:
AB_2844811
Cite Format: Affinity Biosciences Cat# DF12006, RRID:AB_2844811.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C2orf33; Chromosome 2 open reading frame 33; DKFZp666J168; GL004; Mff; MFF_HUMAN; MGC110913; Mitochondrial fission factor; OTTHUMP00000164235;

Immunogens

Immunogen:
Uniprot:

Q9GZY8(MFF_HUMAN) >>Visit HPA database.

Gene(ID):
MFF(56947)
Expression:
Q9GZY8 MFF_HUMAN:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

Sequence:
MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Xenopus
50
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZY8 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
Y35 Phosphorylation
Y39 Phosphorylation
T40 Phosphorylation
K54 Ubiquitination
S74 Phosphorylation
S93 Phosphorylation
T106 Phosphorylation
K109 Ubiquitination
K114 Ubiquitination
T115 Phosphorylation
T121 Phosphorylation
S123 Phosphorylation
T137 Phosphorylation
T138 Phosphorylation
S155 Phosphorylation Q13131 (PRKAA1)
S157 Phosphorylation
S172 Phosphorylation Q13131 (PRKAA1)
S177 Phosphorylation
S179 Phosphorylation
T200 Phosphorylation
S202 Phosphorylation
S222 Phosphorylation
S223 Phosphorylation
S229 Phosphorylation
S233 Phosphorylation
S258 Phosphorylation
S263 Phosphorylation
K302 Ubiquitination

Research Backgrounds

Function:

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

Subcellular Location:

Mitochondrion outer membrane>Single-pass type IV membrane protein. Peroxisome. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

Subunit Structure:

Homodimer. Interacts with DNM1L (By similarity).

Family&Domains:

Belongs to the Tango11 family.

References

1). Paeoniflorin Ameliorates Skeletal Muscle Atrophy in Chronic Kidney Disease via AMPK/SIRT1/PGC-1α-Mediated Oxidative Stress and Mitochondrial Dysfunction. Frontiers in Pharmacology, 2022 (PubMed: 35370668) [IF=5.6]

Application: WB    Species: rat    Sample: C2C12 cells

FIGURE 8 | PGC-1α might be important in mediating the effects of PF on TNF-α-treated C2C12 cells.(I) Representative western blots using antibodies against p-DRP1 (Ser616), DRP1, FIS1, MFF, MTFP1 and GAPDH.
2). Valproic acid regulates MIEF1 through MST2-HIPPO to suppress breast cancer growth. Life sciences, 2022 (PubMed: 36126724) [IF=5.2]
3). Elucidation of SIRT-1/PGC-1α-associated mitochondrial dysfunction and autophagy in nonalcoholic fatty liver disease. Lipids in Health and Disease, 2021 (PubMed: 33902605) [IF=4.5]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group

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