DACH1 Antibody - #DF12008
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12008, RRID:AB_2844813.
Fold/Unfold
DACH; Dach1; DACH1_HUMAN; dachshund homolog 1 (Drosophila); Dachshund homolog 1; Dachsund homolog 1; FLJ10138; OTTHUMP00000018494;
Immunogens
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
- Q9UI36 DACH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATVTSTGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINASTGSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELICLPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCTNASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHASNNQHGADSENGDMNSSVGSSDGSWDKETLPSSPSQGPQASITHPRMPGARSLPLSHPLNHLQQSHLLPNGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UI36 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| T578 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| T582 | Phosphorylation | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| K666 | Ubiquitination | Uniprot | |
| K680 | Acetylation | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| T727 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain (By similarity).
Nucleus.
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
Interacts with SIX1, SIX6 and EYA3. Interacts with NCOR1 and HDAC3 through its N-terminus. Interacts with SIN3A through its C-terminus (By similarity). Interacts with SMAD3 and SMAD4.
The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
Belongs to the DACH/dachshund family.
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