ADAM12 Antibody - #DF12017

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Product: ADAM12 Antibody
Catalog: DF12017
Description: Rabbit polyclonal antibody to ADAM12
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 97 kDa,68 kDa; 100kD(Calculated).
Uniprot: O43184
RRID: AB_2844822

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ADAM12 Antibody detects endogenous levels of total ADAM12.
RRID:
AB_2844822
Cite Format: Affinity Biosciences Cat# DF12017, RRID:AB_2844822.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; CAR10; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; Meltrin alpha; Meltrin-alpha; Metalloprotease disintegrin 12 transmembrane; MLTN; MLTNA; OTTHUMP00000046766;

Immunogens

Immunogen:
Uniprot:

O43184(ADA12_HUMAN) >>Visit HPA database.

Gene(ID):
ADAM12(8038)
Expression:
O43184 ADA12_HUMAN:

Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

Sequence:
MAARPLPVSPARALLLALAGALLAPCEARGVSLWNQGRADEVVSASVGSGDLWIPVKSFDSKNHPEVLNIRLQRESKELIINLERNEGLIASSFTETHYLQDGTDVSLARNYTVILGHCYYHGHVRGYSDSAVSLSTCSGLRGLIVFENESYVLEPMKSATNRYKLFPAKKLKSVRGSCGSHHNTPNLAAKNVFPPPSQTWARRHKRETLKATKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDMDKCSVSQDPFTSLHEFLDWRKMKLLPRKSHDNAQLVSGVYFQGTTIGMAPIMSMCTADQSGGIVMDHSDNPLGAAVTLAHELGHNFGMNHDTLDRGCSCQMAVEKGGCIMNASTGYPFPMVFSSCSRKDLETSLEKGMGVCLFNLPEVRESFGGQKCGNRFVEEGEECDCGEPEECMNRCCNATTCTLKPDAVCAHGLCCEDCQLKPAGTACRDSSNSCDLPEFCTGASPHCPANVYLHDGHSCQDVDGYCYNGICQTHEQQCVTLWGPGAKPAPGICFERVNSAGDPYGNCGKVSKSSFAKCEMRDAKCGKIQCQGGASRPVIGTNAVSIETNIPLQQGGRILCRGTHVYLGDDMPDPGLVLAGTKCADGKICLNRQCQNISVFGVHECAMQCHGRGVCNNRKNCHCEAHWAPPFCDKFGFGGSTDSGPIRQADNQGLTIGILVTILCLLAAGFVVYLKRKTLIRLLFTNKKTTIEKLRCVRPSRPPRGFQPCQAHLGHLGKGLMRKPPDSYPPKDNPRRLLQCQNVDISRPLNGLNVPQPQSTQRVLPPLHRAPRAPSVPARPLPAKPALRQAQGTCKPNPPQKPLPADPLARTTRLTHALARTPGQWETGLRLAPLRPAPQYPHQVPRSTHTAYIK

PTMs - O43184 As Substrate

Site PTM Type Enzyme
R430 Methylation
R449 Methylation
S782 Phosphorylation
Y783 Phosphorylation
T882 Phosphorylation
Y895 Phosphorylation

Research Backgrounds

Function:

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).

PTMs:

The precursor is cleaved by a furin endopeptidase.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Secreted.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

Subunit Structure:

Interacts with alpha-actinin-2 and with syndecans (By similarity). Interacts with SH3PXD2A. Interacts with FST3. Interacts with RACK1; the interaction is required for PKC-dependent translocation of ADAM12 to the cell membrane.

Family&Domains:

The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

References

1). Differential Gene Expression Profiling in Alveolar Echinococcosis Identifies Potential Biomarkers Associated With Angiogenesis. Open Forum Infectious Diseases, 2023 (PubMed: 36817746) [IF=3.8]

Application: IHC    Species: Human    Sample: AE samples

Figure 5. Immunohistochemical analysis of CD34, vascular endothelial growth factor (VEGF), and angiogenesis-related differentially expressed genes in the experimental (alveolar echinococcosis [AE]) and control (AE control group [AEC]) group samples. (A) CD34 and VEGF staining in AE samples showed different degrees of vascularization. Increased expression of SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 was observed in the AE samples, whereas low or no expression of these genes was observed in the AEC samples. (B) The H-scores of CD34, VEGF, SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 were significantly higher in the AE samples than in the AEC samples (values represent the mean ± standard error of the mean: ∗∗∗P < .001, ∗∗∗∗P < .0001).

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