SRX1 Antibody - #DF12028

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Product: SRX1 Antibody
Catalog: DF12028
Description: Rabbit polyclonal antibody to SRX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 14 kDa; 14kD(Calculated).
Uniprot: Q9BYN0
RRID: AB_2844833

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SRX1 Antibody detects endogenous levels of total SRX1.
RRID:
AB_2844833
Cite Format: Affinity Biosciences Cat# DF12028, RRID:AB_2844833.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C20orf1392; Chromosome 20 open reading frame 139; dJ850E9.2; Npn3; Npn31; SRX1; SRXN 1; Sulfiredoxin 1; Sulfiredoxin 1 homolog (S. cerevisiae); Sulfiredoxin 1 homolog; YKL086W;

Immunogens

Immunogen:
Uniprot:

Q9BYN0(SRXN1_HUMAN) >>Visit HPA database.

Gene(ID):
SRXN1(140809)
Expression:
Q9BYN0 SRXN1_HUMAN:

Widely expressed with highest levels in kidney, lung, spleen and thymus.

Sequence:
MGLRAGGTLGRAGAGRGAPEGPGPSGGAQGGSIHSGRIAAVHNVPLSVLIRPLPSVLDPAKVQSLVDTIREDPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYN0 As Substrate

Site PTM Type Enzyme
R4 Methylation
T8 Phosphorylation
R11 Methylation
R16 Methylation
S32 Phosphorylation
R37 Methylation
S123 Phosphorylation

Research Backgrounds

Function:

Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in kidney, lung, spleen and thymus.

Family&Domains:

Belongs to the sulfiredoxin family.

References

1). Ferroptosis-related gene expression in the pathogenesis of preeclampsia. Frontiers in Genetics, 2022 (PubMed: 36061193) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 6 Diagnostic accuracy analysis and experimental verification of six hub genes. (A–F) ROC curves were plotted to examine the diagnostic potential and discriminatory accuracy of NQO1 (A), SRXN1 (B), CDO1 (C), EGFR (D), FBXW7 (E), and JUN. (F–I) Western blot analysis of NQO1 and SRXN1 protein expression (G), and gray-scale analysis of Western blot analysis results (H–J). Representative images of immunohistochemical staining for NQO1 and SRXN1 in human placenta from normal pregnancy and preeclampsia. (K,L) Statistical analysis of immunohistochemistry results in non-PE placenta tissues (n = 15) and PE placenta tissues (n = 15). IHC means immunohistochemistry. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars = 50 μm in (J).

Application: IHC    Species: Human    Sample:

FIGURE 6 Diagnostic accuracy analysis and experimental verification of six hub genes. (A–F) ROC curves were plotted to examine the diagnostic potential and discriminatory accuracy of NQO1 (A), SRXN1 (B), CDO1 (C), EGFR (D), FBXW7 (E), and JUN. (F–I) Western blot analysis of NQO1 and SRXN1 protein expression (G), and gray-scale analysis of Western blot analysis results (H–J). Representative images of immunohistochemical staining for NQO1 and SRXN1 in human placenta from normal pregnancy and preeclampsia. (K,L) Statistical analysis of immunohistochemistry results in non-PE placenta tissues (n = 15) and PE placenta tissues (n = 15). IHC means immunohistochemistry. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars = 50 μm in (J).

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