CAMSAP2 Antibody - #DF12031

(2)  
Product: CAMSAP2 Antibody
Catalog: DF12031
Description: Rabbit polyclonal antibody to CAMSAP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 168 kDa; 168kD(Calculated).
Uniprot: Q08AD1
RRID: AB_2844836

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CAMSAP2 Antibody detects endogenous levels of total CAMSAP2.
RRID:
AB_2844836
Cite Format: Affinity Biosciences Cat# DF12031, RRID:AB_2844836.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calmodulin regulated spectrin associated protein 1 like 1; Calmodulin regulated spectrin associated protein 2; CAMSAP2; KIAA1078; MGC150680; MGC150681; RP11 93N17.1;

Immunogens

Immunogen:
Uniprot:

Q08AD1(CAMP2_HUMAN) >>Visit HPA database.

Gene(ID):
CAMSAP2(23271)
Sequence:
MGDAADPREMRKTFIVPAIKPFDHYDFSRAKIACNLAWLVAKAFGTENVPEELQEPFYTDQYDQEHIKPPVVNLLLSAELYCRAGSLILKSDAAKPLLGHDAVIQALAQKGLYVTDQEKLVTERDLHKKPIQMSAHLAMIDTLMMAYTVEMVSIEKVIACAQQYSAFFQATDLPYDIEDAVMYWINKVNEHLKDIMEQEQKLKEHHTVEAPGGQKSPSKWFWKLVPARYRKEQTLLKQLPCIPLVENLLKDGTDGCALAALIHFYCPDVVRLEDICLKETMSLADSLYNLQLIQEFCQEYLNQCCHFTLEDMLYAASSIKSNYLVFMAELFWWFEVVKPSFVQPRVVRPQGAEPVKDMPSIPVLNAAKRNVLDSSSDFPSSGEGATFTQSHHHLPSRYSRPQAHSSASGGIRRSSSMSYVDGFIGTWPKEKRSSVHGVSFDISFDKEDSVQRSTPNRGITRSISNEGLTLNNSHVSKHIRKNLSFKPINGEEEAESIEEELNIDSHSDLKSCVPLNTNELNSNENIHYKLPNGALQNRILLDEFGNQIETPSIEEALQIIHDTEKSPHTPQPDQIANGFFLHSQEMSILNSNIKLNQSSPDNVTDTKGALSPITDNTEVDTGIHVPSEDIPETMDEDSSLRDYTVSLDSDMDDASKFLQDYDIRTGNTREALSPCPSTVSTKSQPGSSASSSSGVKMTSFAEQKFRKLNHTDGKSSGSSSQKTTPEGSELNIPHVVAWAQIPEETGLPQGRDTTQLLASEMVHLRMKLEEKRRAIEAQKKKMEAAFTKQRQKMGRTAFLTVVKKKGDGISPLREEAAGAEDEKVYTDRAKEKESQKTDGQRSKSLADIKESMENPQAKWLKSPTTPIDPEKQWNLASPSEETLNEGEILEYTKSIEKLNSSLHFLQQEMQRLSLQQEMLMQMREQQSWVISPPQPSPQKQIRDFKPSKQAGLSSAIAPFSSDSPRPTHPSPQSSNRKSASFSVKSQRTPRPNELKITPLNRTLTPPRSVDSLPRLRRFSPSQVPIQTRSFVCFGDDGEPQLKESKPKEEVKKEELESKGTLEQRGHNPEEKEIKPFESTVSEVLSLPVTETVCLTPNEDQLNQPTEPPPKPVFPPTAPKNVNLIEVSLSDLKPPEKADVPVEKYDGESDKEQFDDDQKVCCGFFFKDDQKAENDMAMKRAALLEKRLRREKETQLRKQQLEAEMEHKKEETRRKTEEERQKKEDERARREFIRQEYMRRKQLKLMEDMDTVIKPRPQVVKQKKQRPKSIHRDHIESPKTPIKGPPVSSLSLASLNTGDNESVHSGKRTPRSESVEGFLSPSRCGSRNGEKDWENASTTSSVASGTEYTGPKLYKEPSAKSNKHIIQNALAHCCLAGKVNEGQKKKILEEMEKSDANNFLILFRDSGCQFRSLYTYCPETEEINKLTGIGPKSITKKMIEGLYKYNSDRKQFSHIPAKTLSASVDAITIHSHLWQTKRPVTPKKLLPTKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08AD1 As Substrate

Site PTM Type Enzyme
K12 Acetylation
S86 Phosphorylation
K95 Ubiquitination
T142 Phosphorylation
S360 Phosphorylation
K368 Methylation
Y398 Phosphorylation
S406 Phosphorylation
S408 Phosphorylation
S414 Phosphorylation
S415 Phosphorylation
S416 Phosphorylation
S418 Phosphorylation
T426 Phosphorylation
S433 Phosphorylation
S434 Phosphorylation
S439 Phosphorylation
S443 Phosphorylation
T454 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation O14757 (CHEK1)
T469 Phosphorylation
S473 Phosphorylation
S476 Phosphorylation
S484 Phosphorylation
S496 Phosphorylation
S522 Phosphorylation
Y528 Phosphorylation
K529 Ubiquitination
S566 Phosphorylation
T569 Phosphorylation
S598 Phosphorylation
S599 Phosphorylation
S611 Phosphorylation
S649 Phosphorylation
S673 Phosphorylation
S683 Phosphorylation
T711 Phosphorylation
S720 Phosphorylation
T724 Phosphorylation
S810 Phosphorylation
S842 Phosphorylation
S844 Phosphorylation
K861 Methylation
S862 Phosphorylation
T864 Phosphorylation
T865 Phosphorylation
S877 Phosphorylation
S900 Phosphorylation
S901 Phosphorylation
S931 Phosphorylation
S936 Phosphorylation
S960 Phosphorylation
S963 Phosphorylation
T967 Phosphorylation
S970 Phosphorylation
S973 Phosphorylation
K977 Acetylation
K977 Methylation
S978 Phosphorylation
S980 Phosphorylation
K984 Methylation
S985 Phosphorylation
T988 Phosphorylation
T997 Phosphorylation
T1002 Phosphorylation
T1004 Phosphorylation
S1008 Phosphorylation
S1011 Phosphorylation
R1014 Methylation
S1019 Phosphorylation
S1021 Phosphorylation
S1029 Phosphorylation
Y1144 Phosphorylation
S1148 Phosphorylation
K1170 Acetylation
K1178 Acetylation
T1250 Phosphorylation
S1268 Phosphorylation
S1276 Phosphorylation
T1279 Phosphorylation
S1290 Phosphorylation
S1293 Phosphorylation
S1304 Phosphorylation
T1308 Phosphorylation
S1311 Phosphorylation
S1313 Phosphorylation
S1319 Phosphorylation
S1321 Phosphorylation
T1338 Phosphorylation
S1339 Phosphorylation
S1340 Phosphorylation
T1348 Phosphorylation
K1354 Ubiquitination
Y1413 Phosphorylation
Y1415 Phosphorylation

Research Backgrounds

Function:

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In addition, it also reduces the velocity of microtubule polymerization. Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes. Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation. Through the microtubule cytoskeleton, regulates the autophagosome transport.

Subcellular Location:

Cytoplasm>Cytoskeleton. Golgi apparatus.
Note: Associated with the minus-end of microtubules and also detected at the centrosomes (PubMed:23169647, PubMed:24486153, PubMed:24908486, PubMed:24706919, PubMed:27666745, PubMed:28814570). Decorates the minus-end of microtubules by decreasing the rate of tubulin incorporation and remaining bound (PubMed:24486153). The length of CAMSAP2-decorated stretches on the minus-end of microtubules depends on MAPRE1/EB1 and MAPRE3/EB3, which promote elongation of CAMSAP2-decorated microtubule stretches (PubMed:28814570). Recruited to the Golgi apparatus by AKAP9 and PDE4DIP isoform 13/MMG8/SMYLE (PubMed:27666745). In neurons, localizes to the minus-end of microtubules in axon and dendrites (PubMed:24908486).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CAMSAP3 (By similarity). Interacts with KATNA1 and KATNB1; leading to regulate the length of CAMSAP2-decorated microtubule stretches. Interacts with a complex formed by AKAP9 and PDE4DIP isoform 13/MMG8/SMYLE, which recruits CAMSAP2 to the Golgi. Interacts with MAPRE1/EB1.

Family&Domains:

The CKK domain binds microtubules and specifically recognizes the minus-end of microtubules (PubMed:24486153).

The MBD (microtubule-binding domain) region can recognize some features of the microtubule lattice, which might contribute to the specific decoration of growing microtubule minus-ends by CAMSAP2.

Belongs to the CAMSAP1 family.

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