PPP1R15B Antibody - #DF12050
(8)
| Product: | PPP1R15B Antibody |
| Catalog: | DF12050 |
| Description: | Rabbit polyclonal antibody to PPP1R15B |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 100-110 kDa; 79kD(Calculated). |
| Uniprot: | Q5SWA1 |
| RRID: | AB_2844855 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(82%), Chicken(82%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PPP1R15B Antibody detects endogenous levels of total PPP1R15B.
RRID:
AB_2844855
Cite Format: Affinity Biosciences Cat# DF12050, RRID:AB_2844855.
Cite Format: Affinity Biosciences Cat# DF12050, RRID:AB_2844855.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
CREP; FLJ14744; PPP1R15B; Protein phosphatase 1, regulatory (inhibitor) subunit 15B; Protein phosphatase 1, regulatory subunit 15B;
Immunogens
Immunogen:
Uniprot:
Sequence:
- Q5SWA1 PR15B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPENSGNPTLLSSAQPETRVSYWTKLLSQLLAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGVYSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPLDWLEEGIHWQYSPPDLKLELKAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNIQRIDNFSVVSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIPASWQGCPPLSTEGLPEIHHLRMKRLEFLQQANKGQDLPTPDQDNGYHSLEEEHSLLRMDPKHCRDNPTQFVPAAGDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSKTPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDAIGYCLTFEHRERMFNRLQGTCFKGLNVLKQC
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
88
Zebrafish
88
Dog
82
Chicken
82
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5SWA1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K35 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| Y650 | Phosphorylation | Uniprot | |
| Y651 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K711 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1.
Subunit Structure:
Part of a complex containing PPP1R15B, PP1 and NCK1/2 (By similarity). Interacts with PP1.
Family&Domains:
Belongs to the PPP1R15 family.
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