CRBN Antibody - #DF12054
| Product: | CRBN Antibody |
| Catalog: | DF12054 |
| Description: | Rabbit polyclonal antibody to CRBN |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat, Monkey |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 51-55 kDa; 51kD(Calculated). |
| Uniprot: | Q96SW2 |
| RRID: | AB_2844859 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12054, RRID:AB_2844859.
Fold/Unfold
2610203G15Rik; 2900045O07Rik; AF229032; AW108261; Cereblon; Crbn; CRBN_HUMAN; DKFZp781K0715; MGC27358; MRT2A; OTTHUMP00000209555; piL; Protein cereblon; Protein x 0001;
Immunogens
- Q96SW2 CRBN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96SW2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| C441 | S-Nitrosylation | Uniprot |
Research Backgrounds
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
Cytoplasm. Nucleus. Membrane>Peripheral membrane protein.
Widely expressed. Highly expressed in brain.
Interacts with KCNT1 (By similarity). Component of a DCX (DDB1-CUL4-X-box) protein ligase complex, at least composed of CRBN, CUL4A, DDB1 and RBX1. Interacts directly with DDB1. Interacts (in pomalidomide-bound form) with IKZF1 and IKZF3.
The CULT domain binds thalidomide and related drugs, such as pomalidomide and lenalidomide. Drug binding leads to a change in substrate specificity of the human DCX (DDB1-CUL4-X-box) E3 protein ligase complex, while no such change is observed in rodents.
Belongs to the CRBN family.
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