ODF2 Antibody - #DF12059

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Product: ODF2 Antibody
Catalog: DF12059
Description: Rabbit polyclonal antibody to ODF2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67-100 kDa; 95kD(Calculated).
Uniprot: Q5BJF6
RRID: AB_2844864

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ODF2 Antibody detects endogenous levels of total ODF2.
RRID:
AB_2844864
Cite Format: Affinity Biosciences Cat# DF12059, RRID:AB_2844864.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

84 kDa outer dense fiber protein; Cancer/testis antigen 134; Cenexin 1; Cenexin; Cenexin1 variant 1; CT134; FLJ44866; KKT 4; KKT4; MGC111096; MGC9034; ODF 2; ODF 84; odf2; ODF2/1; ODF2/2; ODF84; ODFP2_HUMAN; OTTHUMP00000022274; OTTHUMP00000022275; Outer dense fiber of sperm tail 2; Outer dense fiber of sperm tails 2; Outer dense fiber of sperm tails protein 2; Outer dense fiber of sperm tails, 84 kD; Outer dense fiber protein 2; Sperm outer dense fiber major protein 2; Sperm tail structural protein; Testis specific autoantigen;

Immunogens

Immunogen:
Uniprot:

Q5BJF6(ODFP2_HUMAN) >>Visit HPA database.

Gene(ID):
ODF2(4957)
Expression:
Q5BJF6 ODFP2_HUMAN:

Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.

Sequence:
MSASSSGGSPRFPSCGKNGVTSLTQKKVLRAPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKVPWMPPGKSSARPVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQKGERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSKLVEAEMDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSERLMEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQAQLRSKEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYAEQLHVQLADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSLVERLHRQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLERCDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRSRIEHQGDKLEMAREKHQASQKENKQLSLKVDELERKLEATSAQNIEFLQVIAKREEAIHQSQLRLEEKTRECGTLARQLESAIEDARRQVEQTKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
50
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5BJF6 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
K49 Ubiquitination
T52 Phosphorylation
S58 Phosphorylation
K72 Ubiquitination
K81 Ubiquitination
T92 Phosphorylation
S106 Phosphorylation
S109 Phosphorylation
T110 Phosphorylation
S115 Phosphorylation
K119 Ubiquitination
K126 Ubiquitination
S129 Phosphorylation
K138 Ubiquitination
S139 Phosphorylation
K159 Ubiquitination
K190 Ubiquitination
T193 Phosphorylation
K197 Ubiquitination
K198 Ubiquitination
K204 Ubiquitination
K210 Ubiquitination
K223 Ubiquitination
K229 Ubiquitination
T231 Phosphorylation
K239 Ubiquitination
T248 Phosphorylation
K253 Ubiquitination
K259 Ubiquitination
S261 Phosphorylation
K295 Ubiquitination
S302 Phosphorylation
K304 Ubiquitination
K329 Ubiquitination
K333 Ubiquitination
S339 Phosphorylation
K340 Ubiquitination
K368 Ubiquitination
K389 Ubiquitination
K482 Ubiquitination
K505 Ubiquitination
K521 Ubiquitination
K537 Ubiquitination
K572 Methylation
K572 Ubiquitination
K575 Acetylation
K580 Methylation
K604 Ubiquitination
K609 Ubiquitination
K623 Ubiquitination
S632 Phosphorylation
K689 Ubiquitination
K743 Ubiquitination
S792 Phosphorylation
S820 Phosphorylation

Research Backgrounds

Function:

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

PTMs:

Tyrosine phosphorylated. Phosphorylated by TSSK4 on Ser-95.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Spindle pole. Cell projection>Cilium>Flagellum.
Note: Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.

Subunit Structure:

Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts via its C-terminus with PLK1. Interacts with ODF1 (By similarity). Interacts with MARK4; the interaction is required for localization of ODF2 to centrioles. Interacts with TSSK4 (By similarity). Interacts with AKNA (By similarity). Interacts with QRICH2.

Family&Domains:

Belongs to the ODF2 family.

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