GDI2 Antibody - #DF12074

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Product: GDI2 Antibody
Catalog: DF12074
Description: Rabbit polyclonal antibody to GDI2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 48 kDa; 51kD(Calculated).
Uniprot: P50395
RRID: AB_2844879

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GDI2 Antibody detects endogenous levels of total GDI2.
RRID:
AB_2844879
Cite Format: Affinity Biosciences Cat# DF12074, RRID:AB_2844879.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GDI-2; GDI2; GDIB_HUMAN; GDP dissociation inhibitor 2; Guanosine diphosphate dissociation inhibitor 2; Rab GDI beta; Rab GDP dissociation inhibitor beta; RABGDIB;

Immunogens

Immunogen:
Uniprot:

P50395(GDIB_HUMAN) >>Visit HPA database.

Gene(ID):
GDI2(2665)
Expression:
P50395 GDIB_HUMAN:

Ubiquitous.

Sequence:
MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGSPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANFDEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQLICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50395 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y5 Phosphorylation
K29 Ubiquitination
R35 Methylation
Y38 Phosphorylation
Y53 Phosphorylation
K54 Acetylation
K54 Ubiquitination
K57 Ubiquitination
S61 Phosphorylation
R68 Methylation
R70 Methylation
K79 Ubiquitination
Y93 Phosphorylation
T94 Phosphorylation
Y111 Phosphorylation
K112 Acetylation
K112 Methylation
K112 Ubiquitination
Y117 Phosphorylation
S121 Phosphorylation
T122 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K153 Acetylation
K153 Ubiquitination
K164 Acetylation
K164 Ubiquitination
K174 Ubiquitination
Y203 Phosphorylation
T205 Phosphorylation
K210 Ubiquitination
S215 Phosphorylation
K221 Acetylation
K221 Methylation
K221 Ubiquitination
S222 Phosphorylation
Y224 Phosphorylation
Y226 Phosphorylation
Y229 Phosphorylation
Y245 Phosphorylation
K253 Ubiquitination
K269 Acetylation
K269 Sumoylation
K269 Ubiquitination
K278 Ubiquitination
S285 Phosphorylation
K288 Acetylation
K288 Ubiquitination
K293 Ubiquitination
S305 Phosphorylation
K309 Ubiquitination
S316 Phosphorylation
K329 Ubiquitination
S330 Phosphorylation
Y333 Phosphorylation
Y349 Phosphorylation
T355 Phosphorylation
K360 Ubiquitination
K364 Acetylation
K364 Ubiquitination
K379 Ubiquitination
S382 Phosphorylation
S384 Phosphorylation
K390 Ubiquitination
T394 Phosphorylation
S396 Phosphorylation
S401 Phosphorylation
K418 Ubiquitination
K422 Ubiquitination
T425 Phosphorylation
S427 Phosphorylation
K435 Acetylation
K435 Ubiquitination
K438 Ubiquitination
Y442 Phosphorylation

Research Backgrounds

Function:

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with RHOH. Interacts with the non-phosphorylated forms of RAB3A, RAB3B, RAB3C, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43; binds RAB3D to a lesser extent.

Family&Domains:

Belongs to the Rab GDI family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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