HEPACAM Antibody - #DF12075

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Product: HEPACAM Antibody
Catalog: DF12075
Description: Rabbit polyclonal antibody to HEPACAM
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 46-72 kDa; 46kD(Calculated).
Uniprot: Q14CZ8
RRID: AB_2844880

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HEPACAM Antibody detects endogenous levels of total HEPACAM.
RRID:
AB_2844880
Cite Format: Affinity Biosciences Cat# DF12075, RRID:AB_2844880.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ25530; GlialCAM; HECAM_HUMAN; HEPACAM; Hepatocyte cell adhesion molecule; Protein hepaCAM;

Immunogens

Immunogen:
Uniprot:

Q14CZ8(HECAM_HUMAN) >>Visit HPA database.

Gene(ID):
HEPACAM(220296)
Sequence:
MKRERGALSRASRALRLAPFVYLLLIQTDPLEGVNITSPVRLIHGTVGKSALLSVQYSSTSSDRPVVKWQLKRDKPVTVVQSIGTEVIGTLRPDYRDRIRLFENGSLLLSDLQLADEGTYEVEISITDDTFTGEKTINLTVDVPISRPQVLVASTTVLELSEAFTLNCSHENGTKPSYTWLKDGKPLLNDSRMLLSPDQKVLTITRVLMEDDDLYSCMVENPISQGRSLPVKITVYRRSSLYIILSTGGIFLLVTLVTVCACWKPSKRKQKKLEKQNSLEYMDQNDDRLKPEADTLPRSGEQERKNPMALYILKDKDSPETEENPAPEPRSATEPGPPGYSVSPAVPGRSPGLPIRSARRYPRSPARSPATGRTHSSPPRAPSSPGRSRSASRTLRTAGVHIIREQDEAGPVEISA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
80
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14CZ8 As Substrate

Site PTM Type Enzyme
S278 Phosphorylation
T295 Phosphorylation
S318 Phosphorylation
Y340 Phosphorylation
S350 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
T371 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
S383 Phosphorylation
S384 Phosphorylation
S415 Phosphorylation

Research Backgrounds

Function:

Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.

PTMs:

N-glycosylated.

Subcellular Location:

Cytoplasm. Membrane>Single-pass type I membrane protein>Cytoplasmic side.
Note: In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep-G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface. Part of a complex containing MLC1, TRPV4, AQP4 and ATP1B1.

Family&Domains:

The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.

References

1). Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation. Nature communications, 2023 (PubMed: 37620511) [IF=16.6]

Application: WB    Species: Mouse    Sample:

Fig. 2 Involvement of A-Exo. surface signals in promoting axon growth and identification of the surface expression of HepaCAM (GlialCAM) on A-Exo. Representative images (a) and quantification (b) of axon length of cortical neurons in control (i) or treated with proteinase K (10 μg/ml, 5 min) digested A-Exo. (ii) sonicated (30 s) and proteinase K digested A-Exo. (iii) A-Exo. (iv) sonicated A-Exo. (v) or sonicated (30 s) and RNase (10 μg/ml, 5 min) digested A-Exo. (vi) 1 μg A-Exo./sample was used in each treatment in (a, b). White arrows: elongated axons; Number of neurons quantified in each group shown in the graph (6–11 neurons/replicate, 3 biological replicates)/group; Scale bar: 100 μm; Representative images (c) and quantification (d) of axon length of cortical neurons plated on either poly-D-lysine (PDL) coated or PDL/A-Exo. coated coverslips. Number of neurons quantified in each group shown in the graph (10–11 neurons/replicate, 3 biological replicates)/group; Scale bar: 100 μm; e Quantification of axon length of cortical neurons following A-Exo. treatment or co-treatment with A-Exo. and dynasore (dynamin inhibitor, 50 μM). n = 22 (control) or 21 (A-Exo. and A-Exo. + dynasore) neurons (6–7 neurons/replicate, 3 biological replicates)/group; f Proteomic identification of different categories of transmembrane proteins on A-Exo. surface. Specific transmembrane proteins are included in Supplementary Table 1. n = 3 biological replicates; g Detection of specific HepaCAM immunoreactivity (>5 replicates) from spinal cord lysate (10 μg/lane), astrocyte lysate (10 μg/lane), and A-Exo. (1 μg/lane) prepared from WT (+/+), HepaCAM heterozygous (+/−), and HepaCAM KO (−/−) mice; Red arrow: specific HepaCAM immunoreactivity; Black arrow: non-specific immunoreactivity; h Detection of specific HepaCAM immunoreactivity (>5 replicates) in A-Exo. but not in exosome-free ACM fractions; 1 μg A-Exo. was used in each experiment. p value in (d) determined from two-tailed t-test; p values in (b, e) determined by one-way ANOVA followed by post hoc Tukey’s test. Data are presented as mean values ± SEM.

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