Kindlin 2 Antibody - #DF12078

(1)   (8)  
Product: Kindlin 2 Antibody
Catalog: DF12078
Description: Rabbit polyclonal antibody to Kindlin 2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 78 kDa; 78kD(Calculated).
Uniprot: Q96AC1
RRID: AB_2844883

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Kindlin 2 Antibody detects endogenous levels of total Kindlin 2.
RRID:
AB_2844883
Cite Format: Affinity Biosciences Cat# DF12078, RRID:AB_2844883.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ34213; FLJ44462; UNC112; FERM2_HUMAN; Fermitin family homolog 2; Fermt2; KIND2; Kindlin-2; MIG-2; MIG2; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; Pleckstrin homology domain containing family C (with FERM domain) member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112B;

Immunogens

Immunogen:
Uniprot:

Q96AC1(FERM2_HUMAN) >>Visit HPA database.

Gene(ID):
FERMT2(10979)
Expression:
Q96AC1 FERM2_HUMAN:

Ubiquitous. Found in numerous tumor tissues.

Sequence:
MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96AC1 As Substrate

Site PTM Type Enzyme
T32 Phosphorylation
T36 Phosphorylation
K51 Ubiquitination
K56 Acetylation
S59 Phosphorylation
K67 Ubiquitination
K68 Acetylation
K81 Ubiquitination
K89 Ubiquitination
K97 Ubiquitination
K107 Ubiquitination
R118 Methylation
K128 Ubiquitination
T129 Phosphorylation
K142 Ubiquitination
K154 Ubiquitination
S159 Phosphorylation
T172 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
Y179 Phosphorylation
S180 Phosphorylation
S181 Phosphorylation
Y185 Phosphorylation
S186 Phosphorylation
T192 Phosphorylation
Y193 Phosphorylation P12931 (SRC)
S199 Phosphorylation
T204 Phosphorylation
K238 Ubiquitination
K245 Ubiquitination
K247 Ubiquitination
S258 Phosphorylation
K285 Acetylation
K285 Ubiquitination
S339 Phosphorylation
S351 Phosphorylation
Y378 Phosphorylation
K390 Ubiquitination
Y395 Phosphorylation
S403 Phosphorylation
K410 Ubiquitination
S414 Phosphorylation
T416 Phosphorylation
R424 Methylation
S435 Phosphorylation
K438 Ubiquitination
S523 Phosphorylation
K528 Ubiquitination
K533 Ubiquitination
T536 Phosphorylation
S550 Phosphorylation
K582 Ubiquitination
Y590 Phosphorylation
S610 Phosphorylation
S666 Phosphorylation

Research Backgrounds

Function:

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.

Subcellular Location:

Cytoplasm. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell junction>Focal adhesion. Membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus. Cytoplasm>Myofibril>Sarcomere>I band. Cell surface.
Note: Colocalizes with actin stress fibers at cell-ECM focal adhesion sites. Colocalizes with ITGB3 at lamellipodia at the leading edge of spreading cells. Binds to membranes that contain phosphatidylinositides.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Found in numerous tumor tissues.

Subunit Structure:

Interacts with ILK (By similarity). Interacts with FBLIM1. Interacts with ITGB1 and ITGB3. Interacts with active, unphosphorylated CTNNB1. Identified in a complex with CTNNB1 and TCF7L2/TCF4. Interacts with ITGB1; the interaction is inhibited in presence of ITGB1BP1.

Family&Domains:

The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

The PH domain binds phospholipids. Binds preferentially phosphatidylinositol-3,4,5-trisphosphate, and has lower affinity for phosphatidylinositol-4,5-bisphosphate (PubMed:22030399).

The N-terminal region displays a ubiquitin-type fold and mediates interaction with membranes containing negatively charged phosphatidylinositol phosphate via a surface enriched in positively charged residues.

Belongs to the kindlin family.

References

1). YBX1 knockdown induces renal cell carcinoma cell apoptosis via Kindlin-2. Cell Cycle, 2021 (PubMed: 34709966) [IF=3.4]

Application: WB    Species: Human    Sample: renal cancer cells

Figure 1. YBX1 regulates the expression of Kindlin-2 in renal cancer cells. (a) GSEA analyzes genes positively or negatively regulated by YBX1, and the enrichment map shows that the differentially expressed genes are significantly related to EMT. (b) The heat map shows differently expressed genes after YBX1 knockdown during the EMT process. (c) The mRNA levels of Kindlin-2 were examined by qRT-PCR in YBX1 knockdown ACHN (left panel) and 786-O (right-panel) cells. (d) Kindlin-2 protein expressions in YBX1 knockdown ACHN (left panel) and 786-O (right panel) cells were detected by western blot assay. Scr: down-expression empty plasmid control; shYBX1: YBX1 knockdown; Statistically significant differences were indicated

Application: IHC    Species: Human    Sample: renal cancer cells

Figure 2. YBX1 and Kindlin-2 are elevated in RCC and high YBX1 expression correlates with poor prognosis in renal cancer. (a) Relative expression of YBX1 and Kindlin-2 proteins in 20 pairs of RCC tissues (T) and adjacent normal tissues (N) using western blot assay. Upper panel: representative four pairs of western blot images. Lower panel: the expressions of YBX1 and Kindlin-2 was quantified by normalizing with β-actin. The analysis was used paired t-test, P = 0.0059, P = 0.0334, respectively, n = 20. Representative images of immunohistochemical staining for YBX1 and Kindlin-2 are shown in (b). Scale bar = 50 μm. (c) High YBX1 expression correlates with poor prognosis in RCC patients. (http://www.oncolnc.org/, https://kmplot.com/analysis/) Statistically significant differences were indicated:

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.