Piezo1 Antibody - #DF12083

(16)   (15)  
Product: Piezo1 Antibody
Catalog: DF12083
Description: Rabbit polyclonal antibody to Piezo1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 287 kDa; 287kD(Calculated).
Uniprot: Q92508
RRID: AB_2844888

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Piezo1 Antibody detects endogenous levels of total Piezo1.
RRID:
AB_2844888
Cite Format: Affinity Biosciences Cat# DF12083, RRID:AB_2844888.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHS; Fam38a; Family with sequence similarity 38 member A; KIAA0233; Membrane protein induced by beta-amyloid treatment; Mib; PIEZ1_HUMAN; Piezo-type mechanosensitive ion channel component 1; PIEZO1; Protein FAM38A; Protein FAM38B; Protein PIEZO1;

Immunogens

Immunogen:
Uniprot:

Q92508(PIEZ1_HUMAN) >>Visit HPA database.

Gene(ID):
PIEZO1(9780)
Expression:
Q92508 PIEZ1_HUMAN:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

Sequence:
MEPHVLGAVLYWLLLPCALLAACLLRFSGLSLVYLLFLLLLPWFPGPTRCGLQGHTGRLLRALLGLSLLFLVAHLALQICLHIVPRLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGICGRLARNTRQSPHPRELDDDERDVDASPTAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLLFLALCTWWACHFPISTRGFSRLCVAVGCFGAGHLICLYCYQMPLAQALLPPAGIWARVLGLKDFVGPTNCSSPHALVLNTGLDWPVYASPGVLLLLCYATASLRKLRAYRPSGQRKEAAKGYEARELELAELDQWPQERESDQHVVPTAPDTEADNCIVHELTGQSSVLRRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKWAESPAALTEVTVADTEPTRTQTLLQSLGELVKGVYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSLPGTRLPRWAHRQDAVSGTPLLREEQQEHQQQQQEEEEEEEDSRDEGLGVATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPFPNSTNLLPTEISQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHYRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFLVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTGFCWVIQLFSLVCTVKGYYDPKEMMDRDQDCLLPVEEAGIIWDSVCFFFLLLQRRVFLSHYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRIEEKSLAQLKRQMERIRAKQEKHRQGRVDRSRPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAVPEDPRPSAQSAFQLAYQAWVTNAQAVLRRRQQEQEQARQEQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHVVQRVLSTAQFLWMLGQALVDELTRWLQEFTRHHGTMSDVLRAERYLLTQELLQGGEVHRGVLDQLYTSQAEATLPGPTEAPNAPSTVSSGLGAEEPLSSMTDDMGSPLSTGYHTRSGSEEAVTDPGEREAGASLYQGLMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEIAVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGEEEQGAEEGPGVPAATTEDHIQVEARVGPTDGTPEPQVELRPRDTRRISLRFRRRKKEGPARKGAAAIEAEDREEEEGEEEKEAPTGREKRPSRSGGRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFNQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
92
Xenopus
83
Sheep
67
Zebrafish
64
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92508 As Substrate

Site PTM Type Enzyme
K113 Ubiquitination
S165 Phosphorylation
T167 Phosphorylation
T175 Phosphorylation
T179 Phosphorylation
K346 Ubiquitination
K540 Ubiquitination
S732 Phosphorylation
T734 Phosphorylation
S758 Phosphorylation
Y821 Phosphorylation
T822 Phosphorylation
T1016 Phosphorylation
T1027 Phosphorylation
Y1165 Phosphorylation
Y1195 Phosphorylation
K1267 Acetylation
K1334 Ubiquitination
K1345 Ubiquitination
K1351 Ubiquitination
K1360 Ubiquitination
K1381 Ubiquitination
S1391 Phosphorylation
S1395 Phosphorylation
S1396 Phosphorylation
T1479 Phosphorylation
S1509 Phosphorylation
T1510 Phosphorylation
S1619 Phosphorylation
S1621 Phosphorylation
T1626 Phosphorylation
S1636 Phosphorylation
Y1638 Phosphorylation
T1644 Phosphorylation
S1646 Phosphorylation
S1820 Phosphorylation
T1854 Phosphorylation
K1903 Ubiquitination
K1953 Ubiquitination
N2294 N-Glycosylation
K2313 Ubiquitination
K2372 Ubiquitination
K2479 Ubiquitination

Research Backgrounds

Function:

Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane>Multi-pass membrane protein. Cell projection>Lamellipodium membrane.
Note: In erythrocytes, located in the plasma membrane (PubMed:22529292, PubMed:23479567). Accumulates at the leading apical lamellipodia of endothelial cells in response to shear stress (PubMed:25119035).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

Subunit Structure:

Homotrimer. Interacts with PKD2. Interacts with STOML3.

Family&Domains:

Belongs to the PIEZO (TC 1.A.75) family.

References

1). Gastric mechanosensitive channel Piezo1 regulates ghrelin production and food intake. Nature metabolism, 2024 (PubMed: 38467889) [IF=20.8]
2). Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration. Molecular Therapy, 2022 (PubMed: 35619555) [IF=12.1]

Application: WB    Species: Human    Sample: NP tissues

Figure 5. Mechano-stress initiates the positive loop of NF-kB and periostin via PIEZO1 (A) Immunoblotting results of three control and three degenerated human NP tissues. C, control; D, degenerated. (B) Quantitation of Ca2+ influx changes in hNPCs after 5 mM Yoda1 stimulation (left panel), and representative fluorescence images at indicated time points (right panel). Yoda1 was administrated at 10 s. (C) Representative images of Fluo-8 AM in hNPCs cultured on different hydrogels. Scale bar = 100 mm. (D) Representative b-Gal staining images of hNPCs treated with vehicle or Yoda1 for 24 h (left panel), and quantitation of b-Gal+ senescent cells (right panel). n = 3 biologically independent samples. Scale bar, 100 mm. (E–G) qPCR results of senescence (E), SASP (F), and matrix metabolism (G) markers in hNPCs treated with vehicle or Yoda1 for 24 h. n = 3 biologically independent samples. (H) Representative immunostaining images of NF-kB p65 (red) in hNPCs under indicated treatments for 30 min. DAPI (blue) indicates nuclei. (I) Quantification of p65 localization in (H), presented as mean ± SD. In total, 237 cells from the vehicle group and 232 cells from the Yoda1 group were analyzed. *Represents the statistical analysis was conducted in terms of the proportion of N > C between the two groups and the p < 0.05. n = 3 biologically independent samples. Scale bar = 25 mm. (J) Immunoblotting results of hNPCs treated with vehicle or Yoda1 for 48 h. (K and L) Representative b-Gal staining images of hNPCs under indicated conditions for 24 h (K), and quantitation of b-Gal+ senescent cells (L). n = 3 biologically independent samples. Scale bar, 100 mm. (M) Relative mRNA expression of the matrix metabolism and senescence markers and POSTN in hNPCs under indicated conditions. n = 3 biologically independent samples. (N and O) Representative T2WI image (N) and MRI Pfirrmann grade (O) of rat tail IVDs with indicated administration. n = 9 discs.
3). LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX. Redox biology, 2024 (PubMed: 39260063) [IF=10.7]
4). Zuogui pill disrupt the malignant cycle in breast cancer bone metastasis through the Piezo1-Notch-1-GPX4 pathway and active molecules fishing. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38103318) [IF=6.7]
5). Activation of Piezo1 or TRPV2 channels inhibits human ureteral contractions via NO release from the mucosa. Frontiers in Pharmacology, 2024 [IF=5.6]

Application: IF/ICC    Species: Human    Sample:

FIGURE 1. mRNA and protein expression patterns of mechano-sensitive channels in human ureter. (A) Relative mRNA expression of mechano-sensitive channels in intact human proximal ureter or mucosa. Total RNA was extracted from intact proximal ureter or mucosa for RT-qPCR. The primer sets for amplification of the four channels are shown in Table 1. In view of the significantly lower expression of the four channels relative to beta-actin, mRNA expression in each case was determined relative to Piezo1, which showed the highest expression. Summary data are the average values of six experiments. Expression patterns were similar in intact ureter and mucosa. (B) Immunofluorescence labeling showing that TRPV2, TRPV4, Piezo1, and Piezo 2 are mainly expressed on the urothelium of ureter. Urothelial cells were marked with the specific urothelial marker AE1/AE3. The scale bar = 100 μm.
6). Gsmtx4 Alleviated Osteoarthritis through Piezo1/Calcineurin/NFAT1 Signaling Axis under Excessive Mechanical Strain. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36835440) [IF=5.6]

Application: IF/ICC    Species: human and rat    Sample:

Figure 1. Piezo1 was up-regulated in the articular cartilage of OA patients and rats. (a,c) Representative images of HE, Safranin-O/Fast Green staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and OARSI scores (n = 5). (b,d) Immunohistochemistry staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and the percentage of Piezo1 positive cells (n = 5). (e) Immunofluorescence staining and quantified results of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h (n = 3). (f,g) RT-qPCR and Western blot analysis of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h. * p < 0.05; ** p < 0.01; ns, no significant differences. Mann–Whitney U test for (a,c), Student’s t-test for (b,d), one-way ANOVA with Bonferroni’s test for (e–g); scale bar: 100 μm.

Application: WB    Species: human and rat    Sample:

Figure 1. Piezo1 was up-regulated in the articular cartilage of OA patients and rats. (a,c) Representative images of HE, Safranin-O/Fast Green staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and OARSI scores (n = 5). (b,d) Immunohistochemistry staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and the percentage of Piezo1 positive cells (n = 5). (e) Immunofluorescence staining and quantified results of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h (n = 3). (f,g) RT-qPCR and Western blot analysis of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h. * p < 0.05; ** p < 0.01; ns, no significant differences. Mann–Whitney U test for (a,c), Student’s t-test for (b,d), one-way ANOVA with Bonferroni’s test for (e–g); scale bar: 100 μm.
7). Fibrotic matrix induces mesenchymal transformation of epithelial cells in oral submucous fibrosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2023 (PubMed: 34528373) [IF=5.3]
8). Substrate stiffness modulates osteogenic and adipogenic differentiation of osteosarcoma through PIEZO1 mediated signaling pathway. Cellular signalling, 2025 (PubMed: 39798771) [IF=4.8]
9). Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head. Frontiers in cell and developmental biology, 2023 (PubMed: 37876552) [IF=4.6]

Application: IHC    Species: Rat    Sample:

FIGURE 4 In (A), the immunohistochemical results of Piezo1, RUNX2, BMP2, CD31, HIF-a, and VEGF are presented. Additionally, (B–G) display the corresponding quantitative immunohistochemical results. In comparison to the model group, both the blank and WBVT groups exhibited significantly higher expression levels of Piezo1, RUNX2, BMP2, CD31, HIF-a, and VEGF. These differences were statistically significant (p < 0.05).
10). Trimethylamine-N-oxide sensitizes chondrocytes to mechanical loading through the upregulation of Piezo1. FOOD AND CHEMICAL TOXICOLOGY, 2023 (PubMed: 36925039) [IF=3.9]
Load more

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.