Piezo1 Antibody - #DF12083

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Product: Piezo1 Antibody
Catalog: DF12083
Description: Rabbit polyclonal antibody to Piezo1
Application: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 287 kDa; 287kD(Calculated).
Uniprot: Q92508
RRID: AB_2844888

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Piezo1 Antibody detects endogenous levels of total Piezo1.
RRID:
AB_2844888
Cite Format: Affinity Biosciences Cat# DF12083, RRID:AB_2844888.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHS; Fam38a; Family with sequence similarity 38 member A; KIAA0233; Membrane protein induced by beta-amyloid treatment; Mib; PIEZ1_HUMAN; Piezo-type mechanosensitive ion channel component 1; PIEZO1; Protein FAM38A; Protein FAM38B; Protein PIEZO1;

Immunogens

Immunogen:

A synthesized peptide derived from human Piezo1, corresponding to a region within the internal amino acids.

Uniprot:

Q92508(PIEZ1_HUMAN) >>Visit HPA database.

Gene(ID):
PIEZO1(9780)
Expression:
Q92508 PIEZ1_HUMAN:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

Sequence:
MEPHVLGAVLYWLLLPCALLAACLLRFSGLSLVYLLFLLLLPWFPGPTRCGLQGHTGRLLRALLGLSLLFLVAHLALQICLHIVPRLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGICGRLARNTRQSPHPRELDDDERDVDASPTAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLLFLALCTWWACHFPISTRGFSRLCVAVGCFGAGHLICLYCYQMPLAQALLPPAGIWARVLGLKDFVGPTNCSSPHALVLNTGLDWPVYASPGVLLLLCYATASLRKLRAYRPSGQRKEAAKGYEARELELAELDQWPQERESDQHVVPTAPDTEADNCIVHELTGQSSVLRRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKWAESPAALTEVTVADTEPTRTQTLLQSLGELVKGVYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSLPGTRLPRWAHRQDAVSGTPLLREEQQEHQQQQQEEEEEEEDSRDEGLGVATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPFPNSTNLLPTEISQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHYRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFLVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTGFCWVIQLFSLVCTVKGYYDPKEMMDRDQDCLLPVEEAGIIWDSVCFFFLLLQRRVFLSHYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRIEEKSLAQLKRQMERIRAKQEKHRQGRVDRSRPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAVPEDPRPSAQSAFQLAYQAWVTNAQAVLRRRQQEQEQARQEQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHVVQRVLSTAQFLWMLGQALVDELTRWLQEFTRHHGTMSDVLRAERYLLTQELLQGGEVHRGVLDQLYTSQAEATLPGPTEAPNAPSTVSSGLGAEEPLSSMTDDMGSPLSTGYHTRSGSEEAVTDPGEREAGASLYQGLMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEIAVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGEEEQGAEEGPGVPAATTEDHIQVEARVGPTDGTPEPQVELRPRDTRRISLRFRRRKKEGPARKGAAAIEAEDREEEEGEEEKEAPTGREKRPSRSGGRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFNQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
92
Xenopus
83
Sheep
67
Zebrafish
64
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane>Multi-pass membrane protein. Cell projection>Lamellipodium membrane.
Note: In erythrocytes, located in the plasma membrane (PubMed:22529292, PubMed:23479567). Accumulates at the leading apical lamellipodia of endothelial cells in response to shear stress (PubMed:25119035).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

Family&Domains:

Belongs to the PIEZO (TC 1.A.75) family.

References

1). A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotype. Cell discovery, 2022 (PubMed: 36068215) [IF=33.5]

Application: WB    Species: Rat    Sample:

Fig. 1: Enhanced expression of Piezo1 and integrin β1 arising from tissue stiffening during the onset of cardiac fibrosis. a Meta-analysis of published GEO data analysis showing Piezo1, integrin, collagen and connective tissue growth factor (CTGF) expression in lung fibrosis (bleomycin-mediated lung fibrosis, “Bleo”) and heart with sham and injury. b Correlation of Piezo1 and integrin β1 expression in the lung and heart with sham and fibrosis in GEO data. c Representative images of Masson’s trichrome-stained sections from the negative control (NC) and MI rats. Scale bar, 5 mm (LV: left ventricular). d Elastic modulus of the heart sections from NC and MI rats. The elastic modulus of the heart tissue increases from 4.35 kPa (NC) to 8.38 kPa (MI-7d) finally to 16.22 kPa (MI-28d). e Immunohistochemical staining of α-SMA, integrin β1, and Piezo1 in the cardiac tissues of NC and MI rats. Scale bar, 200 μm. f Relative protein levels of α-SMA and Piezo1 determined by Western blot analysis. g RT-PCR analysis of Col 1, α-SMA, TGF-β1, PDGFR-α, TCF21, Piezo1, and integrin β1 in NC and MI rats.

Application: IHC    Species: Rat    Sample:

Fig. 1: Enhanced expression of Piezo1 and integrin β1 arising from tissue stiffening during the onset of cardiac fibrosis. a Meta-analysis of published GEO data analysis showing Piezo1, integrin, collagen and connective tissue growth factor (CTGF) expression in lung fibrosis (bleomycin-mediated lung fibrosis, “Bleo”) and heart with sham and injury. b Correlation of Piezo1 and integrin β1 expression in the lung and heart with sham and fibrosis in GEO data. c Representative images of Masson’s trichrome-stained sections from the negative control (NC) and MI rats. Scale bar, 5 mm (LV: left ventricular). d Elastic modulus of the heart sections from NC and MI rats. The elastic modulus of the heart tissue increases from 4.35 kPa (NC) to 8.38 kPa (MI-7d) finally to 16.22 kPa (MI-28d). e Immunohistochemical staining of α-SMA, integrin β1, and Piezo1 in the cardiac tissues of NC and MI rats. Scale bar, 200 μm. f Relative protein levels of α-SMA and Piezo1 determined by Western blot analysis. g RT-PCR analysis of Col 1, α-SMA, TGF-β1, PDGFR-α, TCF21, Piezo1, and integrin β1 in NC and MI rats.

Application: IF/ICC    Species: Rat    Sample:

Fig. 2: Matrix stiffness-mediated increases of Piezo1 and integrin β1 promote CF activation in vitro. a Schematic of different stages of cardiac fibrosis. In injured regions, accumulation of ECM proteins promotes tissue stiffening. b Moduli of peptide-modified PEG-MAL hydrogels with increasing concentration of PEG-SH. PEG-SH was swollen into the network (2.5% of PEG-MAL) at 1%, 1.125%, 1.25%, 1.5%, and 1.625%, resulting in hydrogels with stiffness of 4, 6, 8, 9, and 15 kPa, respectively. Elastic modulus was measured by rotational rheometry. c Immunofluorescence analysis indicated enhanced activation of CFs with increasing matrix stiffness (gray, Piezo1; green, α-SMA; Pink, Integrin β1). Scale bar, 50 μm. d RT-PCR analysis of α-SMA, PDGFR-α, TGF-β1 and Col 1 in CFs after 7-day culture. e Quantifications of integrin β1 plaque size on matrices of different elastic modulus (n ≥ 90 data points). f Immunofluorescence and RT-PCR analyses of α-SMA, Col 1 and PDGFR-α indicated that activation of CFs decreased following siRNA silencing of Piezo1 (green, α-SMA). Scale bar, 50 μm. g Immunofluorescence and RT-PCR analyses of α-SMA, Col 1 and PDGFR-α indicated that activation of CFs decreased in integrin β1-depleted CFs, both with and without integrin-blocking antibodies (green, α-SMA). Scale bar, 50 μm.
2). Gastric mechanosensitive channel Piezo1 regulates ghrelin production and food intake. Nature metabolism, 2024 (PubMed: 38467889) [IF=20.8]
3). Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration. Journal of nanobiotechnology, 2025 (PubMed: 40069791) [IF=12.6]
4). Piezo1 induces Wnt7b+ astrocytes transformation to modulate glial scar stiffness and neuro-regeneration after stroke. Theranostics, 2026 (PubMed: 41346705) [IF=12.4]
5). Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration. Molecular Therapy, 2022 (PubMed: 35619555) [IF=12.1]

Application: WB    Species: Human    Sample: NP tissues

Figure 5. Mechano-stress initiates the positive loop of NF-kB and periostin via PIEZO1 (A) Immunoblotting results of three control and three degenerated human NP tissues. C, control; D, degenerated. (B) Quantitation of Ca2+ influx changes in hNPCs after 5 mM Yoda1 stimulation (left panel), and representative fluorescence images at indicated time points (right panel). Yoda1 was administrated at 10 s. (C) Representative images of Fluo-8 AM in hNPCs cultured on different hydrogels. Scale bar = 100 mm. (D) Representative b-Gal staining images of hNPCs treated with vehicle or Yoda1 for 24 h (left panel), and quantitation of b-Gal+ senescent cells (right panel). n = 3 biologically independent samples. Scale bar, 100 mm. (E–G) qPCR results of senescence (E), SASP (F), and matrix metabolism (G) markers in hNPCs treated with vehicle or Yoda1 for 24 h. n = 3 biologically independent samples. (H) Representative immunostaining images of NF-kB p65 (red) in hNPCs under indicated treatments for 30 min. DAPI (blue) indicates nuclei. (I) Quantification of p65 localization in (H), presented as mean ± SD. In total, 237 cells from the vehicle group and 232 cells from the Yoda1 group were analyzed. *Represents the statistical analysis was conducted in terms of the proportion of N > C between the two groups and the p < 0.05. n = 3 biologically independent samples. Scale bar = 25 mm. (J) Immunoblotting results of hNPCs treated with vehicle or Yoda1 for 48 h. (K and L) Representative b-Gal staining images of hNPCs under indicated conditions for 24 h (K), and quantitation of b-Gal+ senescent cells (L). n = 3 biologically independent samples. Scale bar, 100 mm. (M) Relative mRNA expression of the matrix metabolism and senescence markers and POSTN in hNPCs under indicated conditions. n = 3 biologically independent samples. (N and O) Representative T2WI image (N) and MRI Pfirrmann grade (O) of rat tail IVDs with indicated administration. n = 9 discs.
6). Built-In Electric Field Accelerates Nanotopography-Mediated Enhancement of Vascularized Osseointegration via Cav1.2/Piezo/Ca2+/PI3K Signaling. Small science, 2025 (PubMed: 41058722) [IF=11.1]

Application: IF/ICC    Species: human    Sample:

Figure 7 NBT and NBTP surfaces promote HUVEC spreading, proliferation, and angiogenesis. A) Confocal fluorescence images of HUVEC cytoskeleton (F-actin, yellow) and nuclei (blue) after 24 h of culture on different surfaces. B) Semiquantitative analysis of cell aspect ratio. C) Semiquantitative analysis of nucleus-to-cytoplasm ratio. D) CCK-8 proliferation assay on each surface after seeding for 1, 4, and 7 days. E) Tube formation assay after 7 days of culture. F) Semiquantitative analysis of tube formation parameters. G) mRNA expression of angiogenesis-related genes (eNOS, VEGF, HIF-1α). H) Immunofluorescence staining of CD31, VEGF, and eNOS and corresponding semiquantitative analysis. I) Immunofluorescent staining of Piezo1, vinculin, YAP, and Ca2+ and corresponding semiquantitative analysis. ANOVA followed by Tukey's post hoc test was performed for statistical analysis
7). LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX. Redox biology, 2024 (PubMed: 39260063) [IF=10.7]
8). Inhalable microplastics of different shapes disrupt airway epithelial homeostasis: A comparative study of fibers and irregular particles. Environment international, 2025 (PubMed: 40845405) [IF=10.3]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 6. Polyacrylonitrile exposure alters ciliary structure and Piezo1 expression in airway epithelial cells. (A) The representative immunofluorescence images of cilia in different groups. Arl13b, red fluorescence; DAPI, blue fluorescence. (B) Representative immunofluorescence images of Piezo1 expression in mouse bronchial epithelium across experimental groups. Piezo1, red fluorescence; DAPI, blue fluorescence. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
9). Mechanically tunable fiber-based hydrogel activates PIEZO1-integrin axis for enhanced bone repair. Journal of nanobiotechnology, 2025 (PubMed: 40903759) [IF=10.2]

Application: IF/ICC    Species: Rat    Sample:

Fig. 7 OHADN fiber@Yoda1 hydrogel promoted alveolar bone regeneration in vivo. (a) Immunohistochemical staining and quantification of average optical density for RUNX2 and OPN of the alveolar bone defect. (b) Immunohistochemical staining and quantification of average optical density for PIEZO1, ITGα5 and TNS1 of the alveolar bone defect. (c) Immunohistochemical co-staining of PIEZO1 (green) and ITGα5 (red). Yellow indicates their spatial co-localization. Right panel: Quantitative curve of the fluorescence assay analyzed by Image J software. (**P

Application: IHC    Species: Rat    Sample:

Fig. 7 OHADN fiber@Yoda1 hydrogel promoted alveolar bone regeneration in vivo. (a) Immunohistochemical staining and quantification of average optical density for RUNX2 and OPN of the alveolar bone defect. (b) Immunohistochemical staining and quantification of average optical density for PIEZO1, ITGα5 and TNS1 of the alveolar bone defect. (c) Immunohistochemical co-staining of PIEZO1 (green) and ITGα5 (red). Yellow indicates their spatial co-localization. Right panel: Quantitative curve of the fluorescence assay analyzed by Image J software. (**P
10). CXCR4 Clustering Induced by Polymeric Nanothreads Impedes Cancer Cell Metastasis via PIEZO1-Mediated Mechanotransduction. Advanced healthcare materials, 2025 (PubMed: 40567012) [IF=10.0]
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