TET2 Antibody - #DF12089

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Product: TET2 Antibody
Catalog: DF12089
Description: Rabbit polyclonal antibody to TET2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 130 kDa,133 kDa,224 kDa; 224kD(Calculated).
Uniprot: Q6N021
RRID: AB_2844894

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TET2 Antibody detects endogenous levels of total TET2.
RRID:
AB_2844894
Cite Format: Affinity Biosciences Cat# DF12089, RRID:AB_2844894.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ20032; KIAA1546; MDS; Methylcytosine dioxygenase TET2; Nbla00191; Probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Tet 2; Tet methylcytosine dioxygenase 2; Tet oncogene 2; Tet oncogene family member 2; TET2; TET2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q6N021(TET2_HUMAN) >>Visit HPA database.

Gene(ID):
TET2(54790)
Expression:
Q6N021 TET2_HUMAN:

Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.

Sequence:
MEQDRTNHVEGNRLSPFLIPSPPICQTEPLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGEEFCSGSSSNLQAPGGSSERYLKQNEMNGAYFKQSSVFTKDSFSATTTPPPPSQLLLSPPPPLPQVPQLPSEGKSTLNGGVLEEHHHYPNQSNTTLLREVKIEGKPEAPPSQSPNPSTHVCSPSPMLSERPQNNCVNRNDIQTAGTMTVPLCSEKTRPMSEHLKHNPPIFGSSGELQDNCQQLMRNKEQEILKGRDKEQTRDLVPPTQHYLKPGWIELKAPRFHQAESHLKRNEASLPSILQYQPNLSNQMTSKQYTGNSNMPGGLPRQAYTQKTTQLEHKSQMYQVEMNQGQSQGTVDQHLQFQKPSHQVHFSKTDHLPKAHVQSLCGTRFHFQQRADSQTEKLMSPVLKQHLNQQASETEPFSNSHLLQHKPHKQAAQTQPSQSSHLPQNQQQQQKLQIKNKEEILQTFPHPQSNNDQQREGSFFGQTKVEECFHGENQYSKSSEFETHNVQMGLEEVQNINRRNSPYSQTMKSSACKIQVSCSNNTHLVSENKEQTTHPELFAGNKTQNLHHMQYFPNNVIPKQDLLHRCFQEQEQKSQQASVLQGYKNRNQDMSGQQAAQLAQQRYLIHNHANVFPVPDQGGSHTQTPPQKDTQKHAALRWHLLQKQEQQQTQQPQTESCHSQMHRPIKVEPGCKPHACMHTAPPENKTWKKVTKQENPPASCDNVQQKSIIETMEQHLKQFHAKSLFDHKALTLKSQKQVKVEMSGPVTVLTRQTTAAELDSHTPALEQQTTSSEKTPTKRTAASVLNNFIESPSKLLDTPIKNLLDTPVKTQYDFPSCRCVEQIIEKDEGPFYTHLGAGPNVAAIREIMEERFGQKGKAIRIERVIYTGKEGKSSQGCPIAKWVVRRSSSEEKLLCLVRERAGHTCEAAVIVILILVWEGIPLSLADKLYSELTETLRKYGTLTNRRCALNEERTCACQGLDPETCGASFSFGCSWSMYYNGCKFARSKIPRKFKLLGDDPKEEEKLESHLQNLSTLMAPTYKKLAPDAYNNQIEYEHRAPECRLGLKEGRPFSGVTACLDFCAHAHRDLHNMQNGSTLVCTLTREDNREFGGKPEDEQLHVLPLYKVSDVDEFGSVEAQEEKKRSGAIQVLSSFRRKVRMLAEPVKTCRQRKLEAKKAAAEKLSSLENSSNKNEKEKSAPSRTKQTENASQAKQLAELLRLSGPVMQQSQQPQPLQKQPPQPQQQQRPQQQQPHHPQTESVNSYSASGSTNPYMRRPNPVSPYPNSSHTSDIYGSTSPMNFYSTSSQAAGSYLNSSNPMNPYPGLLNQNTQYPSYQCNGNLSVDNCSPYLGSYSPQSQPMDLYRYPSQDPLSKLSLPPIHTLYQPRFGNSQSFTSKYLGYGNQNMQGDGFSSCTIRPNVHHVGKLPPYPTHEMDGHFMGATSRLPPNLSNPNMDYKNGEHHSPSHIIHNYSAAPGMFNSSLHALHLQNKENDMLSHTANGLSKMLPALNHDRTACVQGGLHKLSDANGQEKQPLALVQGVASGAEDNDEVWSDSEQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFIKSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6N021 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S21 Phosphorylation
S38 Phosphorylation
K53 Acetylation
S75 Phosphorylation
T97 Phosphorylation
S99 Phosphorylation Q13131 (PRKAA1)
S102 Phosphorylation
K110 Acetylation
K111 Acetylation
S334 Phosphorylation
R369 Methylation
S585 Phosphorylation
K655 Ubiquitination
S696 Phosphorylation
K747 Ubiquitination
S817 Phosphorylation
Y819 Phosphorylation
S820 Phosphorylation
T822 Phosphorylation
T859 Phosphorylation
K900 Ubiquitination
S1099 Phosphorylation
S1107 Phosphorylation
S1109 Phosphorylation
T1114 Phosphorylation
K1117 Acetylation
T1122 Phosphorylation
K1299 Ubiquitination
K1317 Acetylation
T1331 Phosphorylation
K1338 Ubiquitination
K1339 Acetylation
K1339 Ubiquitination
Y1345 Phosphorylation
S1449 Phosphorylation
T1463 Phosphorylation
K1478 Acetylation
S1481 Phosphorylation
S1494 Phosphorylation
S1497 Phosphorylation
T1502 Phosphorylation
S1506 Phosphorylation
S1518 O-Glycosylation
S1559 Phosphorylation
Y1560 Phosphorylation
S1561 Phosphorylation
R1682 Methylation
T1975 Phosphorylation

Research Backgrounds

Function:

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

PTMs:

May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT. In contrast, another group reports GlcNAcylation by OGT in mouse ortholog.

Tissue Specificity:

Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.

Subunit Structure:

Interacts with HCFC1 and OGT.

Family&Domains:

Belongs to the TET family.

References

1). Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration. Development (Cambridge, England), 2025 (PubMed: 39976298) [IF=3.7]
2). CircDIP2C ameliorates oxidized low-density lipoprotein-induced cell dysfunction by binding to miR-556-5p to induce TET2 in human umbilical vein endothelial cells. VASCULAR PHARMACOLOGY, 2021 (PubMed: 34147657) [IF=3.5]

Application: WB    Species: Human    Sample: umbilical vein endothelial cells

Fig. 5. TET2 expression was modulated by circDIP2C through functioning as a target gene of miR-556-5p in HUVECs. (A) The binding sites between miR-556-5p and TET2 were predicted by targetscan database. (B-D) The binding relationship between miR-556-5p and TET2 was proved by dual-luciferase reporter, RIP and RNA pull-down assays in HUVECs. (E and F) The mRNA and protein expression of TET2 were detected by qRT-PCR or western blot analysis in the HUVECs transfected with miR-NC or miR-556-5p. (G and H) The effects between circDIP2C and miR-556-5p overexpression on the mRNA and protein expression of TET2 were determined by qRT-PCR or western blot analysis in HUVECs. (I and J) The impacts of ox-LDL on the mRNA and protein expression of TET2 were demonstrated by qRT-PCR and western blot analysis, respectively, in HUVECs. *P < 0.05.

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