DEK Antibody - #DF12097
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| Product: | DEK Antibody |
| Catalog: | DF12097 |
| Description: | Rabbit polyclonal antibody to DEK |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 43-50 kDa; 43kD(Calculated). |
| Uniprot: | P35659 |
| RRID: | AB_2844902 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12097, RRID:AB_2844902.
Fold/Unfold
D6S231E; Dek; DEK gene; DEK oncogene; DEK oncogene DNA binding; DEK_HUMAN; Protein DEK;
Immunogens
- P35659 DEK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASAPAAEGEGTPTQPASEKEPEMPGPREESEEEEDEDDEEEEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTIAQGKGQKLCEIERIHFFLSKKKTDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSVQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKTCSKGSKKERNSSGMARKAKRTKCPEILSDESSSDEDEKKNKEESSDDEDKESEEEPPKKTAKREKPKQKATSKSKKSVKSANVKKADSSTTKKNQNSSKKESESEDSSDDEPLIKKLKKPPTDEELKETIKKLLASANLEEVTMKQICKKVYENYPTYDLTERKDFIKTTVKELIS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35659 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| S32 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S51 | Phosphorylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T67 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S71 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| K84 | Methylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| R93 | Methylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| C161 | S-Nitrosylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| K283 | Acetylation | Uniprot | |
| K284 | Acetylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| T289 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| K326 | Acetylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| T342 | Phosphorylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot |
Research Backgrounds
Involved in chromatin organization.
Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
Nucleus.
Note: Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
Ubiquitous. Expressed at relatively high levels.
Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with histones H2A, H2B, H3, H4, acetylated histone H4, non-phosphorylated DAXX and HDAC2. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Binds DNA.
References
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