GOLPH3 Antibody - #DF12099
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| Product: | GOLPH3 Antibody |
| Catalog: | DF12099 |
| Description: | Rabbit polyclonal antibody to GOLPH3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 34 kDa; 34kD(Calculated). |
| Uniprot: | Q9H4A6 |
| RRID: | AB_2844904 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12099, RRID:AB_2844904.
Fold/Unfold
Coat protein; Coat protein GPP34; FLJ90675; Golgi associated protein; Golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3 (coat protein); Golgi phosphoprotein 3; Golgi protein; GOLP3_HUMAN; Golph3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor;
Immunogens
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
- Q9H4A6 GOLP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSLTQRSSGLVQRRTEASRNAADKERAAGGGAGSSEDDAQSRRDEQDDDDKGDSKETRLTLMEEVLLLGLKDREGYTSFWNDCISSGLRGCMLIELALRGRLQLEACGMRRKSLLTRKVICKSDAPTGDVLLDEALKHVKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDRRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECLKANTNEVLWAVVAAFTK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H4A6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| T143 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T148 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| Y168 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| C280 | S-Nitrosylation | Uniprot | |
| K298 | Ubiquitination | Uniprot |
Research Backgrounds
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
Phosphorylated.
Golgi apparatus>Golgi stack membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein>Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome.
Note: Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35.
Belongs to the GOLPH3/VPS74 family.
References
Application: IF/ICC Species: human Sample: Beas-2B cells
Application: WB Species: human Sample: Beas-2B cells
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