AGGF1 Antibody - #DF12109

(3)   (7)  
Product: AGGF1 Antibody
Catalog: DF12109
Description: Rabbit polyclonal antibody to AGGF1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 84-100 kDa; 81kD(Calculated).
Uniprot: Q8N302
RRID: AB_2844914

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(90%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
AGGF1 Antibody detects endogenous levels of total AGGF1.
RRID:
AB_2844914
Cite Format: Affinity Biosciences Cat# DF12109, RRID:AB_2844914.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGGF 1; Aggf1; AGGF1_HUMAN; Angiogenic factor VG5Q; Angiogenic factor with G patch and FHA domains 1; G patch domain containing protein 7; G patch domain-containing protein 7; GPATC 7; GPATC7; GPATCH 7; GPATCH7; HSU84971; HUS84971; hVG5Q; Vasculogenesis gene on 5q; Vasculogenesis gene on 5q protein; VG5Q;

Immunogens

Immunogen:
Uniprot:

Q8N302(AGGF1_HUMAN) >>Visit HPA database.

Gene(ID):
AGGF1(55109)
Expression:
Q8N302 AGGF1_HUMAN:

Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells.

Sequence:
MASEAPSPPRSPPPPTSPEPELAQLRRKVEKLERELRSCKRQVREIEKLLHHTERLYQNAESNNQELRTQVEELSKILQRGRNEDNKKSDVEVQTENHAPWSISDYFYQTYYNDVSLPNKVTELSDQQDQAIETSILNSKDHLQVENDAYPGTDRTENVKYRQVDHFASNSQEPASALATEDTSLEGSSLAESLRAAAEAAVSQTGFSYDENTGLYFDHSTGFYYDSENQLYYDPSTGIYYYCDVESGRYQFHSRVDLQPYPTSSTKQSKDKKLKKKRKDPDSSATNEEKDLNSEDQKAFSVEHTSCNEEENFANMKKKAKIGIHHKNSPPKVTVPTSGNTIESPLHENISNSTSFKDEKIMETDSEPEEGEITDSQTEDSYDEAITSEGNVTAEDSEDEDEDKIWPPCIRVIVIRSPVLQIGSLFIITAVNPATIGREKDMEHTLRIPEVGVSKFHAEIYFDHDLQSYVLVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMPWVKGTLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
90
Xenopus
80
Zebrafish
80
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N302 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S7 Phosphorylation
S11 Phosphorylation
T16 Phosphorylation
S17 Phosphorylation
K48 Ubiquitination
S171 Phosphorylation
S306 Phosphorylation
S329 Phosphorylation
T341 Phosphorylation
S344 Phosphorylation
T445 Phosphorylation
S543 Phosphorylation
S615 Phosphorylation
S620 Phosphorylation
T647 Phosphorylation
K664 Acetylation
K679 Acetylation

Research Backgrounds

Function:

Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion.

Subcellular Location:

Cytoplasm. Secreted.
Note: Cytoplasmic in microvascular endothelial cells. Upon angiogenesis, when endothelial cell tube formation is initiated, it is secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells.

Subunit Structure:

Interacts with the secreted angiogenic factor TNFSF12.

References

1). The Relationship Between UBE2C and AGGF1 Overexpression and Tumor Angiogenesis in Non-Small Cell Lung Cancer. Cancer Management and Research, 2021 (PubMed: 34354374) [IF=2.5]

Application: IHC    Species: Human    Sample: NSCLC tissues

Figure 1 Immunostaining of UBE2C, AGGF1, VM and MVD in NSCLC and control tissues. (A) Positive staining of UBE2C in NSCLC tissues (×400). (B) Negative staining of UBE2C in control tissues (×400). (C) Positive staining of AGGF1 in NSCLC tissues (×400). (D) Negative staining of AGGF1 in control tissues (×400). (E) Positive staining of VM in NSCLC tissues (×400). (F) Negative staining of VM in control tissues (×400). (G) High MVD in NSCLC tissues (×400). (H) Low MVD in control tissues (×400).
2). Expression and Clinical Significance of the NCAPH, AGGF1, and FOXC2 Proteins in Serous Ovarian Cancer. Cancer Management and Research, 2022 (PubMed: 34584452) [IF=2.5]

Application: IHC    Species: Human    Sample: SOC tissue

Figure 1 The expression of the three proteins in SOC and ovarian serous cystadenoma (ElivisionTM, ×400; (A) NCAPH in SOC; proportion of positive cells; (B) NCAPH in ovarian serous cystadenoma; (C) AGGF1 in SOC; (D) AGGF1 in ovarian serous cystadenoma; (E) FOXC2 in SOC; (F) FOXC2 in ovarian serous cystadenoma).
3). The expression of AGGF1, FOXC2, and E-cadherin in esophageal carcinoma and their clinical significance. Medicine, 2020 (PubMed: 32925786) [IF=1.3]

Application: IHC    Species: human    Sample: esophageal squamous cell carcinoma tissue

Figure 1. |Immunostaining for AGGF1 (angiogenic factor with G-patch and FHA domain 1), FOXC2 (forkhead box C2), and E-cad (E-cadherin) in esophageal squamous cell carcinoma and control tissue. A, Positive AGGF1 in the cytoplasm of esophageal squamous cell carcinoma tissue (40 magnification)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.