OSBP Antibody - #DF12128

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Product: OSBP Antibody
Catalog: DF12128
Description: Rabbit polyclonal antibody to OSBP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 89 kDa; 89kD(Calculated).
Uniprot: P22059
RRID: AB_2844933

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
OSBP Antibody detects endogenous levels of total OSBP.
RRID:
AB_2844933
Cite Format: Affinity Biosciences Cat# DF12128, RRID:AB_2844933.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OSBP 1; OSBP; OSBP1_HUMAN; Oxysterol binding protein 1; Oxysterol-binding protein 1;

Immunogens

Immunogen:
Uniprot:

P22059(OSBP1_HUMAN) >>Visit HPA database.

Gene(ID):
OSBP(5007)
Expression:
P22059 OSBP1_HUMAN:

Widely expressed.

Sequence:
MAATELRGVVGPGPAAIAALGGGGAGPPVVGGGGGRGDAGPGSGAASGTVVAAAAGGPGPGAGGVAAAGPAPAPPTGGSGGSGAGGSGSAREGWLFKWTNYIKGYQRRWFVLSNGLLSYYRSKAEMRHTCRGTINLATANITVEDSCNFIISNGGAQTYHLKASSEVERQRWVTALELAKAKAVKMLAESDESGDEESVSQTDKTELQNTLRTLSSKVEDLSTCNDLIAKHGTALQRSLSELESLKLPAESNEKIKQVNERATLFRITSNAMINACRDFLMLAQTHSKKWQKSLQYERDQRIRLEETLEQLAKQHNHLERAFRGATVLPANTPGNVGSGKDQCCSGKGDMSDEDDENEFFDAPEIITMPENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRTRIPYKPNYSLNLWSIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYHELLDRAAKCENSLEQLCYVAAFTVSSYSTTVFRTSKPFNPLLGETFELDRLEENGYRSLCEQVSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLGTIHCIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKFVPYSYFSRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIGENGGDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPTDSRLRPDQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKALWFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Sheep
92
Chicken
92
Bovine
91
Zebrafish
67
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22059 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T4 Phosphorylation
R7 Methylation
K97 Ubiquitination
K103 Acetylation
Y105 Phosphorylation
Y119 Phosphorylation
T129 Phosphorylation
T174 Phosphorylation
S190 Phosphorylation
S193 Phosphorylation
S198 Phosphorylation
S200 Phosphorylation
T202 Phosphorylation
T210 Phosphorylation
T213 Phosphorylation
K217 Ubiquitination
K230 Ubiquitination
T233 Phosphorylation
S238 Phosphorylation
S240 Phosphorylation Q15139 (PRKD1)
K246 Ubiquitination
S269 Phosphorylation
S287 Phosphorylation
K292 Ubiquitination
Y296 Phosphorylation
R323 Methylation
T332 Phosphorylation
S338 Phosphorylation
K340 Ubiquitination
S345 Phosphorylation
S351 Phosphorylation
T377 Phosphorylation
S379 Phosphorylation
S382 Phosphorylation
S385 Phosphorylation
S386 Phosphorylation
S389 Phosphorylation
K395 Ubiquitination
K429 Ubiquitination
K433 Ubiquitination
Y456 Phosphorylation
R462 Methylation
S469 Phosphorylation
K493 Ubiquitination
K544 Ubiquitination
Y553 Phosphorylation
Y573 Phosphorylation
K604 Ubiquitination
T628 Phosphorylation
S635 Phosphorylation
T645 Phosphorylation
T758 Phosphorylation
T762 Phosphorylation
Y764 Phosphorylation
Y767 Phosphorylation
K768 Ubiquitination
T780 Phosphorylation
Y787 Phosphorylation

Research Backgrounds

Function:

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Perinuclear region. Golgi apparatus membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus>trans-Golgi network.
Note: Predominantly cytosolic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Homodimer or homotrimer. Interacts (via FFAT motif) with VAPA. Interacts (via C-terminus) with RELCH (via the third HEAT repeat) (By similarity). Found in a complex composed of RELCH, OSBP1 and RAB11A (By similarity).

Family&Domains:

The FFAT motif is required for interaction with VATA and proper localization of the protein.

The PH and the Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding.

The second coiled-coil domain is required for interaction with the tyrosine phosphatase.

Belongs to the OSBP family.

References

1). Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3. International Journal of Molecular Sciences, 2020 (PubMed: 32054021) [IF=5.6]
2). Proteomic Profile of Silicotic Rats Identifies PTPN2, VRK1, and B-Factor as New Biomarkers of Pneumoconiosis. , 2019

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