Product: TIP47 Antibody
Catalog: DF12139
Description: Rabbit polyclonal antibody to TIP47
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: O60664
RRID: AB_2844944

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
TIP47 Antibody detects endogenous levels of total TIP47.
RRID:
AB_2844944
Cite Format: Affinity Biosciences Cat# DF12139, RRID:AB_2844944.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; Mannose-6-phosphate receptor-binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin-3; Perilipin3; Placental protein 17; PLIN3; PLIN3_HUMAN; pp17; Tail interacting protein 47 kD; TIP47;

Immunogens

Immunogen:

A synthesized peptide derived from human TIP47

Uniprot:
Gene(ID):
Sequence:
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQVLSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK

Research Backgrounds

Function:

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

Subcellular Location:

Cytoplasm. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet.
Note: Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the perilipin family.

References

1). Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver. ACS Applied Materials & Interfaces, 2018 (PubMed: 29521495) [IF=8.3]

Application: IF/ICC    Species:    Sample: HeLa cells

Figure S24|Confocal fluorescence images of HeLa cells co@stained with N@Cy and anti@TIP47 antiserum. (1): DIC; (2): N-Cy; (4): Anti@TIP47 antiserum; (4): Merged image of (2) and (3); (5): Merged image of (1) @ (3). (6): The normalized fluorescence profiles of N-Cy and ER red along the white line in (4).N-Cy: 4 UM, 2 min; λex = 488 nm, λem = 510@540 nm. Anti@TIP47 antiserum (fluorescence from Alexa Fluor 594@conjugated goat anti@rabbit IgG): λex = 561 nm; λem = 600@700 nm. Bar = 20 Um.

Restrictive clause

 

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For Research Use Only.
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