ISCU Antibody - #DF12158
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12158, RRID:AB_2844963.
Fold/Unfold
2310020H20Rik; HML; hnifU; Iron sulfur cluster assembly enzyme ISCU mitochondrial; Iron sulfur cluster scaffold homolog (E. coli); Iron sulfur cluster scaffold homolog; Iron-sulfur cluster assembly enzyme ISCU; Iscu; IscU iron sulfur cluster scaffold homolog; ISCU_HUMAN; ISU2; MGC74517; mitochondrial; NIFU; NifU like N terminal domain containing; NifU like N terminal domain containing protein; NifU like protein; NifU-like N-terminal domain-containing protein; NifU-like protein; NIFUN; Nitrogen fixation cluster like; OTTHUMP00000238760; OTTHUMP00000238761; OTTHUMP00000238762; OTTHUMP00000238764; OTTHUMP00000238765;
Immunogens
Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
- Q9H1K1 ISCU_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H1K1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S20 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot |
Research Backgrounds
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).
Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm.
Mitochondrion.
Cytoplasm. Nucleus.
Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
Binds NFS1. Interacts with HSCB. Interacts with GLRX5. Interacts with HSPA9. Interacts with HSC20.
Belongs to the NifU family.
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