LMOD1 Antibody - #DF12160

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Product: LMOD1 Antibody
Catalog: DF12160
Description: Rabbit polyclonal antibody to LMOD1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 64,85 kDa; 67kD(Calculated).
Uniprot: P29536
RRID: AB_2844965

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Rabbit(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
LMOD1 Antibody detects endogenous levels of total LMOD1.
RRID:
AB_2844965
Cite Format: Affinity Biosciences Cat# DF12160, RRID:AB_2844965.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1D; 64 kDa autoantigen 1D; 64 kDa autoantigen 1D3; 64 kDa autoantigen D1; 9530015K06Rik; D1; Leiomodin; Leiomodin-1; Lmod1; LMOD1_HUMAN; muscle form; SM LMOD; SM-Lmod; SMLMOD; Smooth muscle leiomodin; Thyroid associated ophthalmopathy autoantigen; Thyroid-associated ophthalmopathy autoantigen;

Immunogens

Immunogen:
Uniprot:

P29536(LMOD1_HUMAN) >>Visit HPA database.

Gene(ID):
LMOD1(25802)
Expression:
P29536 LMOD1_HUMAN:

Detected in lung vascular smooth muscle (at protein level) (PubMed:27144530). Detected in thyroid and extraocular smooth muscle, but not skeletal muscle (PubMed:2026759). Detected in heart, aorta, skeletal muscle, colon, urinary bladder, uterus, stomach, and small intestine (PubMed:11318603).

Sequence:
MSRVAKYRRQVSEDPDIDSLLETLSPEEMEELEKELDVVDPDGSVPVGLRQRNQTEKQSTGVYNREAMLNFCEKETKKLMQREMSMDESKQVETKTDAKNGEERGRDASKKALGPRRDSDLGKEPKRGGLKKSFSRDRDEAGGKSGEKPKEEKIIRGIDKGRVRAAVDKKEAGKDGRGEERAVATKKEEEKKGSDRNTGLSRDKDKKREEMKEVAKKEDDEKVKGERRNTDTRKEGEKMKRAGGNTDMKKEDEKVKRGTGNTDTKKDDEKVKKNEPLHEKEAKDDSKTKTPEKQTPSGPTKPSEGPAKVEEEAAPSIFDEPLERVKNNDPEMTEVNVNNSDCITNEILVRFTEALEFNTVVKLFALANTRADDHVAFAIAIMLKANKTITSLNLDSNHITGKGILAIFRALLQNNTLTELRFHNQRHICGGKTEMEIAKLLKENTTLLKLGYHFELAGPRMTVTNLLSRNMDKQRQKRLQEQRQAQEAKGEKKDLLEVPKAGAVAKGSPKPSPQPSPKPSPKNSPKKGGAPAAPPPPPPPLAPPLIMENLKNSLSPATQRKMGDKVLPAQEKNSRDQLLAAIRSSNLKQLKKVEVPKLLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Bovine
92
Chicken
92
Xenopus
75
Dog
71
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29536 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S19 Phosphorylation
S59 Phosphorylation
S85 Phosphorylation
S119 Phosphorylation
S133 Phosphorylation
S135 Phosphorylation
T230 Phosphorylation
T232 Phosphorylation
K240 Methylation
K249 Acetylation
K250 Acetylation
K287 Acetylation
T288 Phosphorylation
T295 Phosphorylation
T300 Phosphorylation
K308 Acetylation
S508 Phosphorylation
S512 Phosphorylation
S516 Phosphorylation
S520 Phosphorylation
S524 Phosphorylation
S555 Phosphorylation
S574 Phosphorylation

Research Backgrounds

Function:

Mediates nucleation of actin filaments.

Subcellular Location:

Cytoplasm>Myofibril>Sarcomere. Cytoplasm>Cytoskeleton.
Note: Colocalizes with actin filaments in sarcomeres.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in lung vascular smooth muscle (at protein level). Detected in thyroid and extraocular smooth muscle, but not skeletal muscle. Detected in heart, aorta, skeletal muscle, colon, urinary bladder, uterus, stomach, and small intestine.

Family&Domains:

Belongs to the tropomodulin family.

References

1). Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB). Clinical Proteomics, 2019 (PubMed: 30988666) [IF=2.8]

Application: WB    Species: human    Sample: ESCC tissues

Fig. 5|The diferentially expressed proteins were validated by Western Blot. Compared with adjacent normal tissues, the protein expression of PTMA, PAK2, PPP1CA, HMGB2 were up-regulated (a, b), and the protein expression of Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1, Vinculin were down-regulated in ESCC tissues from four pairs of samples (c, d). Representative immunoblot images (a, c) and histograms (mean ± SD; b, d).The experiments were repeated at least three times, N represented normal tissues and T represented tumor tissues

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