MCM10 Antibody - #DF12162

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Product: MCM10 Antibody
Catalog: DF12162
Description: Rabbit polyclonal antibody to MCM10
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 97-105 kDa; 98kD(Calculated).
Uniprot: Q7L590
RRID: AB_2844967

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
MCM10 Antibody detects endogenous levels of total MCM10.
RRID:
AB_2844967
Cite Format: Affinity Biosciences Cat# DF12162, RRID:AB_2844967.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CNA43; DNA43; FLJ31133; Homolog of yeast MCM10; HsMCM10; mcm10; MCM10 minichromosome maintenance deficient 10; MCM10_HUMAN; Minichromosome maintenance 10 replication initiation factor; Minichromosome maintenance complex component 10; Minichromosome maintenance protein 10 isoform 1; PRO2249; Protein MCM10 homolog;

Immunogens

Immunogen:
Uniprot:

Q7L590(MCM10_HUMAN) >>Visit HPA database.

Gene(ID):
MCM10(55388)
Sequence:
MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEADDGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPVEKSPRPPLKERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSAPSQPLQTISRNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQIKEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTVVGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Dog
92
Xenopus
92
Zebrafish
92
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L590 As Substrate

Site PTM Type Enzyme
S26 Phosphorylation
S54 Phosphorylation
T85 Phosphorylation
S93 Phosphorylation
S95 Phosphorylation
K110 Ubiquitination
K127 Ubiquitination
K134 Sumoylation
K134 Ubiquitination
K139 Sumoylation
K139 Ubiquitination
S143 Phosphorylation
S150 Phosphorylation
S155 Phosphorylation
T192 Phosphorylation
S196 Phosphorylation
S212 Phosphorylation
T217 Phosphorylation
S219 Phosphorylation
K222 Ubiquitination
T234 Phosphorylation
S238 Phosphorylation
S261 Phosphorylation
T296 Phosphorylation
K303 Ubiquitination
K313 Sumoylation
K313 Ubiquitination
T314 Phosphorylation
K360 Ubiquitination
K362 Sumoylation
K395 Ubiquitination
S435 Phosphorylation
Y460 Phosphorylation
S472 Phosphorylation
K482 Sumoylation
S488 Phosphorylation
K493 Ubiquitination
K505 Ubiquitination
K511 Sumoylation
K511 Ubiquitination
S514 Phosphorylation
K520 Ubiquitination
K534 Sumoylation
K534 Ubiquitination
K539 Ubiquitination
T541 Phosphorylation
S548 Phosphorylation
K554 Methylation
S557 Phosphorylation
K563 Ubiquitination
S593 Phosphorylation
T610 Phosphorylation
S612 Phosphorylation
T625 Phosphorylation
K627 Sumoylation
K627 Ubiquitination
Y641 Phosphorylation
S644 Phosphorylation
K650 Ubiquitination
S652 Phosphorylation
K658 Ubiquitination
K659 Ubiquitination
T664 Phosphorylation
K665 Sumoylation
K665 Ubiquitination
K669 Sumoylation
K675 Sumoylation
K675 Ubiquitination
K682 Sumoylation
K686 Ubiquitination
K695 Sumoylation
K695 Ubiquitination
T702 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
Y725 Phosphorylation
K733 Ubiquitination
K740 Ubiquitination
K746 Sumoylation
K746 Ubiquitination
K762 Sumoylation
K762 Ubiquitination
K769 Sumoylation
K777 Acetylation
K811 Ubiquitination
K815 Ubiquitination
S824 Phosphorylation
R827 Methylation
K831 Ubiquitination
K854 Sumoylation
K854 Ubiquitination
K869 Sumoylation
K869 Ubiquitination

Research Backgrounds

Function:

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38.

Subcellular Location:

Nucleus.
Note: Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Self-associates (By similarity). Interacts with ORC2. May interact with MCM2 and MCM6. Interacts with the DNA polymerase alpha subunit POLA1. Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity. Interacts with WDHD1.

Family&Domains:

Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.

The N-terminal domain mediates homodimerization.

Belongs to the MCM10 family.

References

1). Minichromosome maintenance protein 10 as a marker for proliferation and prognosis in lung cancer. International Journal of Oncology, 2019 (PubMed: 31638210) [IF=4.5]

Application: WB    Species: Human    Sample: A549 cells

Figure 7 - Downregulation of MCM10 induces G1 arrest by suppressing CCND1 expression in vitro. (A) Flow cytometry analysis of cell-cycle in A549 and H661 cells with or without MCM10 depletion. (B) Reverse transcription-quantitative PCR was performed to explore the expression of CDK1, CDK2, CDK4, CDK6, P27, P19, P21, CCNA2, CCND1, CCNB1, CCNE1 in siMCM10 and NC groups of A549 cells. (C) Relative expression of CCND1 after MCM10 knockdown in A549 and H661 cells. (D) Western blotting assay showing MCM10 and CCND1 protein expression level after MCM10 knockdown in A549 and H661 cells. GAPDH was used as a loading control. Data are presented as the mean ± SEM of three independent experiments, each performed in triplicate. *P

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