Melanophilin Antibody - #DF12163

(3)  
Product: Melanophilin Antibody
Catalog: DF12163
Description: Rabbit polyclonal antibody to Melanophilin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Chicken
Mol.Wt.: 66 kDa, 80 kDa; 66kD(Calculated).
Uniprot: Q9BV36
RRID: AB_2844968

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Sheep(83%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
Melanophilin Antibody detects endogenous levels of total Melanophilin.
RRID:
AB_2844968
Cite Format: Affinity Biosciences Cat# DF12163, RRID:AB_2844968.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2210418F23Rik; 5031433I09Rik; AW228792; D1Wsu84e; Exophilin 3; Exophilin-3; l(1)-3Rk; l1Rk3; Leaden; Leaden protein; ln; Melanophilin; MELPH_HUMAN; MGC2771; MGC59733; Mlph; Slac 2a; SlaC2-a; Slp homolog lacking C2 domains a; Synaptotagmin like protein 2a; Synaptotagmin like protein lacking C2 domains A; Synaptotagmin-like protein 2a;

Immunogens

Immunogen:
Uniprot:

Q9BV36(MELPH_HUMAN) >>Visit HPA database.

Gene(ID):
MLPH(79083)
Sequence:
MGKKLDLSKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKAAPHKAEGLEEADTGASGCHSHPEEQPTSISPSRHGALAELCPPGGSHRMALGTAAALGSNVIRNEQLPLQYLADVDTSDEESIRAHVMASHHSKRRGRASSESQIFELNKHISAVECLLTYLENTVVPPLAKGLGAGVRTEADVEEEALRRKLEELTSNVSDQETSSEEEEAKDEKAEPNRDKSVGPLPQADPEVGTAAHQTNRQEKSPQDPGDPVQYNRTTDEELSELEDRVAVTASEVQQAESEVSDIESRIAALRAAGLTVKPSGKPRRKSNLPIFLPRVAGKLGKRPEDPNADPSSEAKAMAVPYLLRRKFSNSLKSQGKDDDSFDRKSVYRGSLTQRNPNARKGMASHTFAKPVVAHQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
90
Bovine
83
Sheep
83
Horse
75
Rabbit
67
Pig
58
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BV36 As Substrate

Site PTM Type Enzyme
K136 Ubiquitination
S140 Phosphorylation
S155 Phosphorylation
S159 Phosphorylation
S162 Phosphorylation
T165 Phosphorylation
S173 Phosphorylation
K185 Acetylation
S191 Phosphorylation
S201 Phosphorylation
S204 Phosphorylation
S223 Phosphorylation
S226 Phosphorylation
T228 Phosphorylation
S231 Phosphorylation
S233 Phosphorylation
S266 Phosphorylation
S295 Phosphorylation
T313 Phosphorylation
S314 Phosphorylation
S318 Phosphorylation
S326 Phosphorylation
S336 Phosphorylation
S337 Phosphorylation
S397 Phosphorylation
T401 Phosphorylation
S402 Phosphorylation
S403 Phosphorylation
S420 Phosphorylation
S444 Phosphorylation
Y454 Phosphorylation
T457 Phosphorylation
T458 Phosphorylation
S463 Phosphorylation
S481 Phosphorylation
S484 Phosphorylation
S510 Phosphorylation
S552 Phosphorylation
S554 Phosphorylation
K560 Ubiquitination
S569 Phosphorylation
Y571 Phosphorylation
S574 Phosphorylation

Research Backgrounds

Function:

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds RAB27A that has been activated by GTP-binding via its N-terminus. Binds MYO5A via its C-terminal coiled coil domain.

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