TRIM44 Antibody - #DF12177

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Product: TRIM44 Antibody
Catalog: DF12177
Description: Rabbit polyclonal antibody to TRIM44
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50-55kd; 38kD(Calculated).
Uniprot: Q96DX7
RRID: AB_2844982

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TRIM44 Antibody detects endogenous levels of total TRIM44.
RRID:
AB_2844982
Cite Format: Affinity Biosciences Cat# DF12177, RRID:AB_2844982.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AN3; DIPB; HSA249128; MC7; MGC3490; Protein DIPB; TRI44_HUMAN; TRIM44; Tripartite motif containing 44; Tripartite motif containing protein 44; Tripartite motif-containing protein 44;

Immunogens

Immunogen:
Uniprot:

Q96DX7(TRI44_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM44(54765)
Expression:
Q96DX7 TRI44_HUMAN:

Highly expressed in testis.

Sequence:
MASGVGAAFEELPHDGTCDECEPDEAPGAEEVCRECGFCYCRRHAEAHRQKFLSHHLAEYVHGSQAWTPPADGEGAGKEEAEVKVEQEREIESEAGEESESEEESESEEESETEEESEDESDEESEEDSEEEMEDEQESEAEEDNQEEGESEAEGETEAESEFDPEIEMEAERVAKRKCPDHGLDLSTYCQEDRQLICVLCPVIGAHQGHQLSTLDEAFEELRSKDSGGLKAAMIELVERLKFKSSDPKVTRDQMKMFIQQEFKKVQKVIADEEQKALHLVDIQEAMATAHVTEILADIQSHMDRLMTQMAQAKEQLDTSNESAEPKAEGDEEGPSGASEEEDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Zebrafish
83
Xenopus
43
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96DX7 As Substrate

Site PTM Type Enzyme
K178 Ubiquitination
K225 Ubiquitination
K231 Ubiquitination
K249 Ubiquitination
K256 Ubiquitination
K264 Ubiquitination
K265 Ubiquitination
K268 Ubiquitination
K314 Ubiquitination
T319 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
S336 Phosphorylation
S339 Phosphorylation

Research Backgrounds

Function:

May play a role in the process of differentiation and maturation of neuronal cells (By similarity). May regulate the activity of TRIM17. Is a negative regulator of PAX6 expression.

Tissue Specificity:

Highly expressed in testis.

Subunit Structure:

Interacts (via coiled coil) with TRIM17 (via coiled coil).

References

1). Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion via targeting miR‐134 in retinoblastoma. JOURNAL OF GENE MEDICINE, 2021 (PubMed: 33818859) [IF=3.2]

Application: WB    Species: Human    Sample: Retinoblastoma (Rb) tissues

FIGURE 5 TRIM44 is a molecular target of miR-134. (A) Schematic of TRIM44 30 -UTR wild-type (WT) and mutant (Mut) luciferase reporter vectors. (B) Verification of TRIM44 as a target gene of miR-134 by the dual-luciferase reporter assay. (C) Detection of TRIM44 mRNA expression in Weri-Rb1, Y79 and ARPE-19. (D) Detection of TRIM44 mRNA-expressing levels in Rb tissues and adjacent normal tissues (n = 50). (E) Linear regression analysis for the correlation between TRIM44 and miR-134 in Rb tissues (n = 50). (F) Detection of TRIM44 protein expression in WeriRb1, Y79, and ARPE-19. (G) Detection of TRIM44 protein expression in Rb and adjacent normal tissues (n = 50). (H) Analysis of TRIM44 protein expression in Weri-Rb1 and Y79 cells transfected with miR-NC or miR-134. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01

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