AFG3L2 Antibody - #DF12186
| Product: | AFG3L2 Antibody |
| Catalog: | DF12186 |
| Description: | Rabbit polyclonal antibody to AFG3L2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Dog, Chicken |
| Mol.Wt.: | 80-90 kDa; 89kD(Calculated). |
| Uniprot: | Q9Y4W6 |
| RRID: | AB_2844991 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12186, RRID:AB_2844991.
Fold/Unfold
AFG3 (ATPase family gene 3, yeast) like 2; AFG3 ATPase family gene 3 like 2 (yeast); AFG3 ATPase family gene 3 like 2; AFG3 like protein 2; AFG3-like protein 2; AFG32_HUMAN; AFG3L2; ATPase family gene 3 like 2; ATPase family gene 3 yeast; EC 3.4.24.-; FLJ25993; Paraplegin like protein; Paraplegin-like protein; SCA28; Spinocerebellar ataxia 28;
Immunogens
- Q9Y4W6 AFG32_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAHRCLRLWGRGGCWPRGLQQLLVPGGVGPGEQPCLRTLYRFVTTQARASRNSLLTDIIAAYQRFCSRPPKGFEKYFPNGKNGKKASEPKEVMGEKKESKPAATTRSSGGGGGGGGKRGGKKDDSHWWSRFQKGDIPWDDKDFRMFFLWTALFWGGVMFYLLLKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRTGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y4W6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K100 | Acetylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K306 | Acetylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K543 | Acetylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| T560 | Phosphorylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| R624 | Methylation | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| K710 | Acetylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S765 | Phosphorylation | Uniprot | |
| T770 | Phosphorylation | Uniprot |
Research Backgrounds
ATP-dependent protease which is essential for axonal and neuron development. In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU. Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form (By similarity). Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP).
Upon import into the mitochondrion, the N-terminal transit peptide is cleaved to generate an intermediate form which undergoes autocatalytic proteolytic processing to generate the proteolytically active mature form.
Mitochondrion. Mitochondrion inner membrane>Multi-pass membrane protein.
Ubiquitous. Highly expressed in the cerebellar Purkinje cells.
Homooligomer. Forms heterooligomers with SPG7 and AFG3L1. Interacts with SPG7; the interaction is required for the efficient assembly of mitochondrial complex I. Interacts with AFG3L1 (By similarity). Interacts with MAIP1.
In the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family.
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