ICT1 Antibody - #DF12205
| Product: | ICT1 Antibody |
| Catalog: | DF12205 |
| Description: | Rabbit polyclonal antibody to ICT1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 21-24 kDa; 24kD(Calculated). |
| Uniprot: | Q14197 |
| RRID: | AB_2845010 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12205, RRID:AB_2845010.
Fold/Unfold
Digestion substraction 1; DS-1; DS1; Ict1; ICT1_HUMAN; Immature colon carcinoma transcript 1; Immature colon carcinoma transcript 1 protein; mitochondrial; Peptidyl-tRNA hydrolase ICT1; Peptidyl-tRNA hydrolase ICT1, mitochondrial;
Immunogens
Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.
- Q14197 ICT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14197 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y43 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| R76 | Methylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot |
Research Backgrounds
Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
Mitochondrion.
Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.
Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.
Belongs to the prokaryotic/mitochondrial release factor family. Mitochondrion-specific ribosomal protein mL62 subfamily.
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