IPO4 Antibody - #DF12207
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12207, RRID:AB_2845012.
Fold/Unfold
Imp4; Imp4b; Importin 4; Importin 4b; Importin-4; Importin-4b; Ipo4; IPO4_HUMAN; Ran binding protein 4; Ran-binding protein 4; RanBP4;
Immunogens
- Q8TEX9 IPO4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESAGLEQLLRELLLPDTERIRRATEQLQIVLRAPAALPALCDLLASAADPQIRQFAAVLTRRRLNTRWRRLAAEQRESLKSLILTALQRETEHCVSLSLAQLSATIFRKEGLEAWPQLLQLLQHSTHSPHSPEREMGLLLLSVVVTSRPEAFQPHHRELLRLLNETLGEVGSPGLLFYSLRTLTTMAPYLSTEDVPLARMLVPKLIMAMQTLIPIDEAKACEALEALDELLESEVPVITPYLSEVLTFCLEVARNVALGNAIRIRILCCLTFLVKVKSKALLKNRLLPPLLHTLFPIVAAEPPPGQLDPEDQDSEEEELEIELMGETPKHFAVQVVDMLALHLPPEKLCPQLMPMLEEALRSESPYQRKAGLLVLAVLSDGAGDHIRQRLLPPLLQIVCKGLEDPSQVVRNAALFALGQFSENLQPHISSYSREVMPLLLAYLKSVPLGHTHHLAKACYALENFVENLGPKVQPYLPELMECMLQLLRNPSSPRAKELAVSALGAIATAAQASLLPYFPAIMEHLREFLLTGREDLQPVQIQSLETLGVLARAVGEPMRPLAEECCQLGLGLCDQVDDPDLRRCTYSLFAALSGLMGEGLAPHLEQITTLMLLSLRSTEGIVPQYDGSSSFLLFDDESDGEEEEELMDEDVEEEDDSEISGYSVENAFFDEKEDTCAAVGEISVNTSVAFLPYMESVFEEVFKLLECPHLNVRKAAHEALGQFCCALHKACQSCPSEPNTAALQAALARVVPSYMQAVNRERERQVVMAVLEALTGVLRSCGTLTLKPPGRLAELCGVLKAVLQRKTACQDTDEEEEEEDDDQAEYDAMLLEHAGEAIPALAAAAGGDSFAPFFAGFLPLLVCKTKQGCTVAEKSFAVGTLAETIQGLGAASAQFVSRLLPVLLSTAQEADPEVRSNAIFGMGVLAEHGGHPAQEHFPKLLGLLFPLLARERHDRVRDNICGALARLLMASPTRKPEPQVLAALLHALPLKEDLEEWVTIGRLFSFLYQSSPDQVIDVAPELLRICSLILADNKIPPDTKAALLLLLTFLAKQHTDSFQAALGSLPVDKAQELQAVLGLS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TEX9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T18 | Phosphorylation | Uniprot | |
| C42 | S-Nitrosylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| T586 | Phosphorylation | Uniprot | |
| C708 | S-Nitrosylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| Y755 | Phosphorylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| T813 | Phosphorylation | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| K875 | Ubiquitination | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| S1081 | Phosphorylation | Uniprot |
Research Backgrounds
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Cytoplasm. Nucleus.
Binds with high affinity to RPS3A. The binding is coupled to RanGTP cycles. Binds to human cytomegalovirus UL84. Found in a cytosolic complex with ASF1A, ASF1B, CDAN1 and histones H3.1 and H4.
Belongs to the importin beta family.
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