MMS19 Antibody - #DF12212
| Product: | MMS19 Antibody |
| Catalog: | DF12212 |
| Description: | Rabbit polyclonal antibody to MMS19 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 103-113 kDa; 113kD(Calculated). |
| Uniprot: | Q96T76 |
| RRID: | AB_2845017 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12212, RRID:AB_2845017.
Fold/Unfold
hMMS19; homolog of yeast MMS19; MET18; MET18 homolog; MET18MMS19 nucleotide excision repair homolog (S. cerevisiae); mms19; MMS19 nucleotide excision repair protein homolog; MMS19 nucleotide excision repair, S. cerevisiae, homolog of; MMS19-like (MET18 homolog, S. cerevisiae); MMS19-like protein; MMS19_HUMAN; MMS19L;
Immunogens
- Q96T76 MMS19_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAAVEAAAPMGALWGLVHDFVVGQQEGPADQVAADVKSGNYTVLQVVEALGSSLENPEPRTRARAIQLLSQVLLHCHTLLLEKEVVHLILFYENRLKDHHLVIPSVLQGLKALSLCVALPPGLAVSVLKAIFQEVHVQSLPQVDRHTVYNIITNFMRTREEELKSLGADFTFGFIQVMDGEKDPRNLLVAFRIVHDLISRDYSLGPFVEELFEVTSCYFPIDFTPPPNDPHGIQREDLILSLRAVLASTPRFAEFLLPLLIEKVDSEVLSAKLDSLQTLNACCAVYGQKELKDFLPSLWASIRREVFQTASERVEAEGLAALHSLTACLSRSVLRADAEDLLDSFLSNILQDCRHHLCEPDMKLVWPSAKLLQAAAGASARACDSVTSNVLPLLLEQFHKHSQSSQRRTILEMLLGFLKLQQKWSYEDKDQRPLNGFKDQLCSLVFMALTDPSTQLQLVGIRTLTVLGAQPDLLSYEDLELAVGHLYRLSFLKEDSQSCRVAALEASGTLAALYPVAFSSHLVPKLAEELRVGESNLTNGDEPTQCSRHLCCLQALSAVSTHPSIVKETLPLLLQHLWQVNRGNMVAQSSDVIAVCQSLRQMAEKCQQDPESCWYFHQTAIPCLLALAVQASMPEKEPSVLRKVLLEDEVLAAMVSVIGTATTHLSPELAAQSVTHIVPLFLDGNVSFLPENSFPSRFQPFQDGSSGQRRLIALLMAFVCSLPRNVEIPQLNQLMRELLELSCCHSCPFSSTAAAKCFAGLLNKHPAGQQLDEFLQLAVDKVEAGLGSGPCRSQAFTLLLWVTKALVLRYHPLSSCLTARLMGLLSDPELGPAAADGFSLLMSDCTDVLTRAGHAEVRIMFRQRFFTDNVPALVQGFHAAPQDVKPNYLKGLSHVLNRLPKPVLLPELPTLLSLLLEALSCPDCVVQLSTLSCLQPLLLEAPQVMSLHVDTLVTKFLNLSSSPSMAVRIAALQCMHALTRLPTPVLLPYKPQVIRALAKPLDDKKRLVRKEAVSARGEWFLLGSPGS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96T76 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| K496 | Acetylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| K888 | Ubiquitination | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S967 | Phosphorylation | Uniprot | |
| K993 | Ubiquitination | Uniprot | |
| S1017 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot |
Research Backgrounds
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery.
Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination by MAGEF1-NSMCE1 ubiquitin ligase complex leading to proteasomal degradation.
Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Ubiquitously expressed with higher expression in testis.
Component of the CIA complex. In the CIA complex, interacts directly with CIAO2B and CIAO3. Component of the MMXD complex, composed of CIAO1, ERCC2, CIAO2B, MMS19 and SLC25A5. Interacts with CIAO2B; the interaction is direct. Interacts with ERCC2/XPD; the interaction is direct. Interacts with ERCC3/XPB and NCOA3/RAC3. Interacts with RTEL1; the interaction mediates the association of RTEL1 with the CIA complex. Interacts with BRIP1.
Belongs to the MET18/MMS19 family.
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