SEPT9 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/6

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DF12229 staining HepG2 by IF/ICC.

DF12229 staining Hela cells by IF/ICC.

Product:	SEPT9 Antibody
Catalog:	DF12229
Description:	Rabbit polyclonal antibody to SEPT9
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	65 kDa,38-40 kDa; 65kD(Calculated).
Uniprot:	Q9UHD8
RRID:	AB_2845034

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

SEPT9 Antibody detects endogenous levels of total SEPT9.

RRID:

AB_2845034
Cite Format: Affinity Biosciences Cat# DF12229, RRID:AB_2845034.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AF17q25; Cell division control protein septin D1; KIAA0991; MLL septin like fusion protein; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; MSF; MSF1; NAPB; Ov/Br septin; Ovarian/breast septin alpha; Ovarian/Breast septin; PNUTL4; SEPT9; SEPT9_HUMAN; SeptD1; Septin 9; Septin D1; Septin-9; SINT1;

Immunogens

Immunogen:

Uniprot:

Q9UHD8(SEPT9_HUMAN) >>Visit HPA database.

Gene(ID):

SEPT9(10801)

Expression:

Q9UHD8 SEPT9_HUMAN:

Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.

Sequence:

Q9UHD8 SEPT9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHD8 As Substrate

Q9UHD8

Site	PTM Type	Source
M1	Acetylation	Uniprot
K2	Methylation	Uniprot
K3	Methylation	Uniprot
S6	Phosphorylation	Uniprot
R10	Methylation	Uniprot
T11	Phosphorylation	Uniprot
S12	Phosphorylation	Uniprot
S13	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
K28	Acetylation	Uniprot
K28	Methylation	Uniprot
K28	Ubiquitination	Uniprot
S30	Phosphorylation	Uniprot
T38	Phosphorylation	Uniprot
S41	Phosphorylation	Uniprot
T42	Phosphorylation	Uniprot
T49	Phosphorylation	Uniprot
S59	Phosphorylation	Uniprot
T60	Phosphorylation	Uniprot
K62	Acetylation	Uniprot
K62	Ubiquitination	Uniprot
S71	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S89	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S111	Phosphorylation	Uniprot
S115	Phosphorylation	Uniprot
K141	Ubiquitination	Uniprot
T142	Phosphorylation	Uniprot
S160	Phosphorylation	Uniprot
K186	Ubiquitination	Uniprot
S203	Phosphorylation	Uniprot
S211	Phosphorylation	Uniprot
S217	Phosphorylation	Uniprot
T235	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
T242	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
C248	S-Nitrosylation	Uniprot
T255	Phosphorylation	Uniprot
K262	Ubiquitination	Uniprot
K271	Ubiquitination	Uniprot
Y278	Phosphorylation	Uniprot
S283	Phosphorylation	Uniprot
K291	Ubiquitination	Uniprot
T317	Phosphorylation	Uniprot
K320	Methylation	Uniprot
K320	Ubiquitination	Uniprot
K322	Methylation	Uniprot
K326	Ubiquitination	Uniprot
S327	Phosphorylation	Uniprot
T331	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot
K338	Ubiquitination	Uniprot
K343	Ubiquitination	Uniprot
K352	Acetylation	Uniprot
K352	Ubiquitination	Uniprot
K357	Ubiquitination	Uniprot
T363	Phosphorylation	Uniprot
K381	Ubiquitination	Uniprot
K389	Ubiquitination	Uniprot
K400	Ubiquitination	Uniprot
K431	Acetylation	Uniprot
K431	Ubiquitination	Uniprot
K435	Ubiquitination	Uniprot
K445	Ubiquitination	Uniprot
T448	Phosphorylation	Uniprot
K458	Ubiquitination	Uniprot
K476	Acetylation	Uniprot
K476	Ubiquitination	Uniprot
S482	Phosphorylation	Uniprot
R485	Methylation	Uniprot
K490	Ubiquitination	Uniprot
S502	Phosphorylation	Uniprot
Y506	Phosphorylation	Uniprot
K511	Acetylation	Uniprot
K511	Ubiquitination	Uniprot
K519	Ubiquitination	Uniprot
T543	Phosphorylation	Uniprot
K549	Ubiquitination	Uniprot

Research Backgrounds

Q9UHD8_SEPT9_HUMAN

Function:

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.

Tissue Specificity:

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments, and microtubules. GTPase activity is required for filament formation. Interacts with SEPTIN2, SEPTIN6, SEPTIN7, SEPTIN11 and SEPTIN14. Interacts with RTKN and ARHGEF18. In a mesenchymal cell line, Rho/RTKN signals cause disruption of wild-type septin filaments, but not of those containing isoform 2 variants HNA Trp-106 and Phe-111. In a mesenchymal cell line, isoform 2 variants HNA Trp-106 and Phe-111, but not wild type, form filaments with SEPTIN4.

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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For Research Use Only.
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SEPT9 Antibody - #DF12229