TLE3 Antibody - #DF12237

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Product: TLE3 Antibody
Catalog: DF12237
Description: Rabbit polyclonal antibody to TLE3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 83 kDa; 83kD(Calculated).
Uniprot: Q04726
RRID: AB_2845042

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TLE3 Antibody detects endogenous levels of total TLE3.
RRID:
AB_2845042
Cite Format: Affinity Biosciences Cat# DF12237, RRID:AB_2845042.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E(sp1) homolog Drosophila; Enhancer of split groucho 3; Enhancer of split groucho-like protein 3; ESG 3; ESG; ESG3; FLJ39460; GRG 3; GRG3; HsT18976; KIAA1547; TLE 3; Tle3; TLE3_HUMAN; Transducin like enhancer of split 3; Transducin like enhancer of split 3 homolog of Drosophila E(sp1); Transducin like enhancer protein 3; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); Transducin-like enhancer protein 3;

Immunogens

Immunogen:
Uniprot:

Q04726(TLE3_HUMAN) >>Visit HPA database.

Gene(ID):
TLE3(7090)
Expression:
Q04726 TLE3_HUMAN:

Placenta and lung.

Sequence:
MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPIGIMASALRTPISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSPMVSFGAVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04726 As Substrate

Site PTM Type Enzyme
Ubiquitination
R6 Methylation
K19 Methylation
K30 Ubiquitination
K49 Ubiquitination
K184 Ubiquitination
S196 Phosphorylation
S197 Phosphorylation
S201 Phosphorylation
S203 Phosphorylation
S205 Phosphorylation
S207 Phosphorylation
S211 Phosphorylation
K213 Acetylation
R215 Methylation
S217 Phosphorylation
Y220 Phosphorylation
S221 Phosphorylation
K232 Acetylation
S234 Phosphorylation
Y238 Phosphorylation
S240 Phosphorylation
S245 Phosphorylation
S253 Phosphorylation
T259 Phosphorylation
S263 Phosphorylation
S267 Phosphorylation
S278 Phosphorylation
T285 Phosphorylation
S286 Phosphorylation
S289 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
S295 Phosphorylation
T296 Phosphorylation
S299 Phosphorylation
K300 Acetylation
K302 Ubiquitination
K309 Acetylation
K309 Ubiquitination
S310 Phosphorylation
S311 Phosphorylation
T312 Phosphorylation
K316 Ubiquitination
S317 Phosphorylation
T319 Phosphorylation
T321 Phosphorylation
T328 Phosphorylation
S332 Phosphorylation
T333 Phosphorylation
T334 Phosphorylation
R338 Methylation
S413 Phosphorylation
S417 Phosphorylation
R430 Methylation
K445 Acetylation
S484 Phosphorylation
K506 Ubiquitination
K510 Ubiquitination
S515 Phosphorylation
S519 Phosphorylation
K520 Ubiquitination
S521 Phosphorylation
K539 Ubiquitination
R643 Methylation
Y694 Phosphorylation
S701 Phosphorylation
S705 Phosphorylation
K720 Acetylation
K720 Ubiquitination
K739 Ubiquitination
K763 Ubiquitination
K764 Sumoylation

Research Backgrounds

Function:

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

PTMs:

Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Placenta and lung.

Subunit Structure:

Homotetramer and heterooligomer with other family members. Binds LEF1, TCF7 and TCF7L1 (By similarity). Binds FOXA2. Interacts with XIAP/BIRC4 and TCF7L2/TCF4. Interacts with TBX18 (via engrailed homology 1 repressor motif), leading to decreased of TBX18 transcriptional activity.

Family&Domains:

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.

Belongs to the WD repeat Groucho/TLE family.

Restrictive clause

 

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