KIFC3 Antibody - #DF12287

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Product: KIFC3 Antibody
Catalog: DF12287
Description: Rabbit polyclonal antibody to KIFC3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 80-85 kDa, 95-100 kDa; 93kD(Calculated).
Uniprot: Q9BVG8
RRID: AB_2845092

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
KIFC3 Antibody detects endogenous levels of total KIFC3.
RRID:
AB_2845092
Cite Format: Affinity Biosciences Cat# DF12287, RRID:AB_2845092.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686D23201; FLJ34694; Kifc3; KIFC3_HUMAN; Kinesin family member C3; Kinesin-like protein KIFC3;

Immunogens

Immunogen:
Uniprot:

Q9BVG8(KIFC3_HUMAN) >>Visit HPA database.

Gene(ID):
KIFC3(3801)
Sequence:
MVPSRRTWNLGATPSLRGLWRVGRAPEPEPGMARPAPAPASPAARPFPHTGPGRLRTGRGKDTPVCGDEDSSARSAARPALAQCRALSVDWAGPGSPHGLYLTLQVEHLKEKLISQAQEVSRLRSELGGTDLEKHRDLLMVENERLRQEMRRCEAELQELRTKPAGPCPGCEHSQESAQLRDKLSQLQLEMAESKGMLSELNLEVQQKTDRLAEVELRLKDCLAEKAQEEERLSRRLRDSHETIASLRAQSPPVKYVIKTVEVESSKTKQALSESQARNQHLQEQVAMQRQVLKEMEQQLQSSHQLTARLRAQIAMYESELERAHGQMLEEMQSLEEDKNRAIEEAFARAQVEMKAVHENLAGVRTNLLTLQPALRTLTNDYNGLKRQVRGFPLLLQEALRSVKAEIGQAIEEVNSNNQELLRKYRRELQLRKKCHNELVRLKGNIRVIARVRPVTKEDGEGPEATNAVTFDADDDSIIHLLHKGKPVSFELDKVFSPQASQQDVFQEVQALVTSCIDGFNVCIFAYGQTGAGKTYTMEGTAENPGINQRALQLLFSEVQEKASDWEYTITVSAAEIYNEVLRDLLGKEPQEKLEIRLCPDGSGQLYVPGLTEFQVQSVDDINKVFEFGHTNRTTEFTNLNEHSSRSHALLIVTVRGVDCSTGLRTTGKLNLVDLAGSERVGKSGAEGSRLREAQHINKSLSALGDVIAALRSRQGHVPFRNSKLTYLLQDSLSGDSKTLMVVQVSPVEKNTSETLYSLKFAERVRSVELGPGLRRAELGSWSSQEHLEWEPACQTPQPSARAHSAPSSGTSSRPGSIRRKLQPSGKSRPLPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Sheep
92
Dog
92
Horse
83
Rabbit
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BVG8 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
T13 Phosphorylation
S41 Phosphorylation
R45 Methylation
R54 Methylation
S88 Phosphorylation
S96 Phosphorylation
K112 Ubiquitination
S115 Phosphorylation
S121 Phosphorylation
K134 Ubiquitination
K163 Ubiquitination
K183 Ubiquitination
K195 Ubiquitination
K220 Ubiquitination
K226 Ubiquitination
S251 Phosphorylation
K259 Ubiquitination
K269 Ubiquitination
K294 Ubiquitination
Y317 Phosphorylation
S334 Phosphorylation
K355 Ubiquitination
K386 Ubiquitination
K404 Ubiquitination
K434 Ubiquitination
K443 Acetylation
K486 Ubiquitination
S557 Phosphorylation
K593 Ubiquitination
K669 Ubiquitination
S781 Phosphorylation
S784 Phosphorylation
S805 Phosphorylation
S808 Phosphorylation
S812 Phosphorylation
S813 Phosphorylation
S817 Phosphorylation

Research Backgrounds

Function:

Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.

Subcellular Location:

Cell junction>Adherens junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasmic vesicle membrane>Peripheral membrane protein.
Note: Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

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