Product: RND3 Antibody
Catalog: DF12311
Description: Rabbit polyclonal antibody to RND3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29 kDa; 27kD(Calculated).
Uniprot: P61587
RRID: AB_2845116

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RND3 Antibody detects endogenous levels of total RND3.
RRID:
AB_2845116
Cite Format: Affinity Biosciences Cat# DF12311, RRID:AB_2845116.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHE; memB; Protein MemB; Ras homolog gene family member E; Rho family GTPase 3; Rho-related GTP-binding protein Rho8; Rho-related GTP-binding protein RhoE; Rho8; RhoE; Rnd3; RND3_HUMAN; Small GTP binding protein Rho8;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P61587 RND3_HUMAN:

Ubiquitous.

Sequence:
MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61587 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation Q13464 (ROCK1)
K9 Ubiquitination
S11 Phosphorylation Q13464 (ROCK1)
K13 Ubiquitination
S14 Phosphorylation
K167 Ubiquitination
T173 Phosphorylation
S210 Phosphorylation Q05513 (PRKCZ)
S218 Phosphorylation Q13464 (ROCK1)
S222 Phosphorylation
S227 Phosphorylation
T231 Phosphorylation
S240 Phosphorylation Q13464 (ROCK1)

Research Backgrounds

Function:

Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Binds ROCK1 (By similarity). Interacts with UBXD5.

Family&Domains:

Belongs to the small GTPase superfamily. Rho family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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