SPG11 Antibody - #DF12322

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Product: SPG11 Antibody
Catalog: DF12322
Description: Rabbit polyclonal antibody to SPG11
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 279 kDa, 220 kDa; 279kD(Calculated).
Uniprot: Q96JI7
RRID: AB_2845127

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
SPG11 Antibody detects endogenous levels of total SPG11.
RRID:
AB_2845127
Cite Format: Affinity Biosciences Cat# DF12322, RRID:AB_2845127.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Colorectal carcinoma associated protein; Colorectal carcinoma-associated protein; DKFZp762B1512; FLJ21439; KIAA1840; Spastic paraplegia 11 (autosomal recessive); Spastic paraplegia 11; Spastic paraplegia 11 protein; Spatacsin; SPG 11; Spg11; SPTCS_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96JI7(SPTCS_HUMAN) >>Visit HPA database.

Gene(ID):
SPG11(80208)
Expression:
Q96JI7 SPTCS_HUMAN:

Expressed in all structures of brain, with a high expression in cerebellum. Expressed in cortical projection neurons.

Sequence:
MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
82
Zebrafish
73
Xenopus
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96JI7 As Substrate

Site PTM Type Enzyme
S148 Phosphorylation
K311 Ubiquitination
K326 Ubiquitination
K329 Ubiquitination
S331 Phosphorylation
K339 Ubiquitination
K350 Ubiquitination
K414 Ubiquitination
K552 Ubiquitination
K561 Ubiquitination
T644 Phosphorylation
K660 Ubiquitination
Y668 Phosphorylation
K676 Ubiquitination
K678 Ubiquitination
S687 Phosphorylation
K739 Ubiquitination
K746 Ubiquitination
K777 Ubiquitination
K819 Ubiquitination
K825 Methylation
K827 Ubiquitination
K835 Ubiquitination
K839 Ubiquitination
K844 Ubiquitination
K876 Ubiquitination
K942 Ubiquitination
T974 Phosphorylation
Y980 Phosphorylation
K981 Ubiquitination
K1024 Ubiquitination
K1110 Ubiquitination
K1117 Ubiquitination
Y1290 Phosphorylation
S1291 Phosphorylation
S1296 Phosphorylation
K1300 Ubiquitination
K1303 Ubiquitination
K1417 Ubiquitination
K1426 Ubiquitination
K1571 Ubiquitination
K1577 Ubiquitination
S1578 Phosphorylation
K1640 Ubiquitination
K1706 Ubiquitination
T1709 Phosphorylation
T1714 Phosphorylation
K1716 Ubiquitination
K1724 Ubiquitination
K1739 Ubiquitination
K1746 Ubiquitination
K1793 Sumoylation
S1827 Phosphorylation
T1828 Phosphorylation
K1853 Ubiquitination
K1863 Ubiquitination
S1955 Phosphorylation
T1956 Phosphorylation
S1957 Phosphorylation
S1958 Phosphorylation
S1961 Phosphorylation
K1963 Ubiquitination
T1966 Phosphorylation
K1983 Ubiquitination
K1988 Ubiquitination
K2022 Ubiquitination
K2045 Ubiquitination
K2071 Ubiquitination
K2099 Ubiquitination
Y2169 Phosphorylation
T2173 Phosphorylation
Y2174 Phosphorylation
K2182 Methylation
K2192 Ubiquitination
K2208 Ubiquitination
S2226 Phosphorylation
K2244 Ubiquitination
K2256 Ubiquitination
K2281 Ubiquitination
K2368 Ubiquitination
K2374 Ubiquitination
K2383 Ubiquitination
Y2404 Phosphorylation

Research Backgrounds

Function:

May play a role in neurite plasticity by maintaining cytoskeleton stability and regulating synaptic vesicle transport.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus. Cell projection>Axon. Cell projection>Dendrite.
Note: Mainly cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all structures of brain, with a high expression in cerebellum. Expressed in cortical projection neurons.

Subunit Structure:

Interacts with AP5Z1, AP5B1, AP5S1 and ZFYVE26.

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