Stanniocalcin 2 Antibody - #DF12324

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Product: Stanniocalcin 2 Antibody
Catalog: DF12324
Description: Rabbit polyclonal antibody to Stanniocalcin 2
Application: WB IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit
Mol.Wt.: 33 kDa; 33kD(Calculated).
Uniprot: O76061
RRID: AB_2845129

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Stanniocalcin 2 Antibody detects endogenous levels of total Stanniocalcin 2.
RRID:
AB_2845129
Cite Format: Affinity Biosciences Cat# DF12324, RRID:AB_2845129.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Stanniocalcin related protein; Stanniocalcin-2; Stanniocalcin-related protein; Stanniocalcin2; STC 2; STC related protein; STC-2; STC-related protein; STC2; STC2_HUMAN; STCRP;

Immunogens

Immunogen:

A synthesized peptide derived from human Stanniocalcin 2, corresponding to a region within C-terminal amino acids.

Uniprot:

O76061(STC2_HUMAN) >>Visit HPA database.

Gene(ID):
STC2(8614)
Expression:
O76061 STC2_HUMAN:

Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

Sequence:
MCAERLGQFMTLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWEDEQSEYSDIRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

Family&Domains:

Belongs to the stanniocalcin family.

References

1). Vimentin, inversely correlating with infiltration of CD8 + T lymphocytes, promotes nuclear translocation of PD-L1 in esophageal squamous cell carcinoma. Biochimica et biophysica acta. Molecular cell research, 2024 (PubMed: 38901494) [IF=4.6]

Application: IHC    Species: Mouse    Sample:

Fig. 6. Blockage of STC-2 and TRAILR2 can recruit more CD8 + T cells in co-culture system. A: IHC examination of STC-2 and TRAILR2 expression in tumor lesions from C57/BL6 mice models. Magnification fold: 40×, Scale bar, 50 μm. ****P < 0.0001, ***P < 0.001, **P < 0.01, as analyzed by two-tailed independent sample Student's t-test. N = 6, each group. B: schema illustrating the co-culture system with Transwell inserts we set up. Dark pink, stands for the supernatant collected from AKR cells. Light pink denotes RPMI 1640 medium containing 3 × 105 murine CD8 + T cells. In antibody intervention group, that is VIM-shRNA-antibody, primary antibodies against STC2 and TRAILR2, respectively, were added at the dilution of 1:50 in the supernatant under Transwell inserts for 4 h. C: qualitative and quantitative analyses of murine CD8 + T cells invading through the pores of Transwell insert. The cell numbers on the images captured were counted using Image J software. *P < 0.05, **P < 0.01, as determined by two-tailed independent sample Student's t-test.

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