STAU1 Antibody - #DF12325

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Product: STAU1 Antibody
Catalog: DF12325
Description: Rabbit polyclonal antibody to STAU1
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 55 kDa; 63kD(Calculated).
Uniprot: O95793
RRID: AB_2845130

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(83%), Rabbit(83%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
STAU1 Antibody detects endogenous levels of total STAU1.
RRID:
AB_2845130
Cite Format: Affinity Biosciences Cat# DF12325, RRID:AB_2845130.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Double stranded RNA binding protein Staufen homolog 1; Double stranded RNA binding protein Staufen homolog; Double-stranded RNA-binding protein Staufen homolog 1; FLJ25010; MGC124588; PPP1R150; STAU; STAU1; STAU1_HUMAN; staufen; Staufen RNA binding protein (Drosophila); Staufen RNA binding protein homolog 1; Staufen, Drosophila, homolog of, 1; Staufen, RNA binding protein, homolog 1 (Drosophila); staufen-like;

Immunogens

Immunogen:

A synthesized peptide derived from human STAU1, corresponding to a region within the internal amino acids.

Uniprot:

O95793(STAU1_HUMAN) >>Visit HPA database.

Gene(ID):
STAU1(6780)
Expression:
O95793 STAU1_HUMAN:

Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.

Sequence:
MSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSASITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
83
Rabbit
83
Chicken
82
Xenopus
67
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.

(Microbial infection) Plays a role in virus particles production of many viruses including of HIV-1, HERV-K, ebola virus and influenza virus. Acts by interacting with various viral proteins involved in particle budding process.

Subcellular Location:

Cytoplasm. Rough endoplasmic reticulum.
Note: Localizes exclusively with the rough reticulum endoplasmic (RER).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.

Family&Domains:

One of the DRDB could be involved in RER binding.

The C-terminal contains the tubulin binding domain (TBD).

References

1). Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Frontiers in Immunology, 2022 (PubMed: 35350787) [IF=5.7]

Application: IHC    Species: Mice    Sample: lung tissues

Figure 9 The expression of SLC27A3 and STAU1 in COPD models. (A) Representative immunohistochemistry images of SLC27A3 in lung tissues of mice. (B) Representative immunohistochemistry images of STAU1 in lung tissues of mice. (C) The expression of SLC27A3 mRNA in lung tissues of mice. (D) The expression of SLC27A3 protein in lung tissues of mice. (E) The expression of STAU1 mRNA in lung tissues of mice. (F) The expression of STAU1 protein in lung tissues of mice. (G) The expression of SLC27A3 mRNA in BEAS-2B cells. (H) The expression of SLC27A3 protein in BEAS-2B cells. (I) The expression of STAU1 mRNA in BEAS-2B cells. (J) The expression of STAU1 protein in BEAS-2B cells. **P < 0.01, ***P < 0.001 vs. Control.

Application: WB    Species: Mice    Sample: lung tissues

Figure 9 The expression of SLC27A3 and STAU1 in COPD models. (A) Representative immunohistochemistry images of SLC27A3 in lung tissues of mice. (B) Representative immunohistochemistry images of STAU1 in lung tissues of mice. (C) The expression of SLC27A3 mRNA in lung tissues of mice. (D) The expression of SLC27A3 protein in lung tissues of mice. (E) The expression of STAU1 mRNA in lung tissues of mice. (F) The expression of STAU1 protein in lung tissues of mice. (G) The expression of SLC27A3 mRNA in BEAS-2B cells. (H) The expression of SLC27A3 protein in BEAS-2B cells. (I) The expression of STAU1 mRNA in BEAS-2B cells. (J) The expression of STAU1 protein in BEAS-2B cells. **P < 0.01, ***P < 0.001 vs. Control.

Application: IHC    Species: mouse    Sample: lung

FIGURE 9 | The expression of SLC27A3 and STAU1 in COPD models. (A) Representative immunohistochemistry images of SLC27A3 in lung tissues of mice. (B) Representative immunohistochemistry images of STAU1 in lung tissues of mice.

Application: WB    Species: mouse    Sample: lung

FIGURE 9 | The expression of SLC27A3 and STAU1 in COPD models. . (F) The expression of STAU1 protein in lung tissues of mice.

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