TRIM9 Antibody - #DF12337
| Product: | TRIM9 Antibody |
| Catalog: | DF12337 |
| Description: | Rabbit polyclonal antibody to TRIM9 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 65-95kD; 79kD(Calculated). |
| Uniprot: | Q9C026 |
| RRID: | AB_2845142 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12337, RRID:AB_2845142.
Fold/Unfold
E3 ubiquitin-protein ligase TRIM9; HGNC:16288; Homolog of rat RING finger Spring; KIAA0282; RING finger protein 91; RING type E3 ubiquitin transferase TRIM9; RNF 91; RNF91; SPRING; TRIM 9; Tripartite motif containing 9; Tripartite motif containing protein 9; Tripartite motif protein 9;
Immunogens
Brain. Highly expressed in the cerebral cortex (at protein level). Severely decreased in the affected brain areas in Parkinson disease and dementia with Lewy bodies.
- Q9C026 TRIM9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSHRAAGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPPATHLSPALAPVPRNSCITCPQCHRSLILDDRGLRGFPKNRVLEGVIDRYQQSKAAALKCQLCEKAPKEATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRVSRRLSPRKVSTCTDHELENHSMYCVQCKMPVCYQCLEEGKHSSHEVKALGAMWKLHKSQLSQALNGLSDRAKEAKEFLVQLRNMVQQIQENSVEFEACLVAQCDALIDALNRRKAQLLARVNKEHEHKLKVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHLTEDQWGKGTLTPRMTTDFDLSLDNSPLLQSIHQLDFVQVKASSPVPATPILQLEECCTHNNSATLSWKQPPLSTVPADGYILELDDGNGGQFREVYVGKETMCTVDGLHFNSTYNARVKAFNKTGVSPYSKTLVLQTSEVAWFAFDPGSAHSDIILSNDNLTVTCSSYDDRVVLGKTGFSKGIHYWELTVDRYDNHPDPAFGVARMDVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTEGGITKGATIGVLLDLNRKNLTFFINDEQQGPIAFDNVEGLFFPAVSLNRNVQVTLHTGLPVPDFYSSRASIA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9C026 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T41 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y619 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.
Auto-ubiquitinated. Poly-ubiquitinated in cultured cells, whereas it is monoubiquitinated in vitro.
Cytoplasm. Cell projection>Dendrite. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle. Cell junction>Synapse. Cytoplasm>Cytoskeleton.
Note: Enriched at synaptic terminals where it exists in a soluble form and a synaptic vesicle-associated form. Associated with the cytoskeleton (By similarity). Found in proximal dendrites of pyramidal neurons in the cerebral cortex and hippocampus, and Purkinje cells in the cerebellum (Ref.7).
Brain. Highly expressed in the cerebral cortex (at protein level). Severely decreased in the affected brain areas in Parkinson disease and dementia with Lewy bodies.
Interacts with SNAP25.
The coiled coil domain mediates the interaction with the N-terminal t-SNARE domain of SNAP25.
Belongs to the TRIM/RBCC family.
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