Antizyme inhibitor 1 Antibody - #DF12348
| Product: | Antizyme inhibitor 1 Antibody |
| Catalog: | DF12348 |
| Description: | Rabbit polyclonal antibody to Antizyme inhibitor 1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 49.5 kDa; 50kD(Calculated). |
| Uniprot: | O14977 |
| RRID: | AB_2845153 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12348, RRID:AB_2845153.
Fold/Unfold
Antizyme inhibitor 1; AZI; Azin1; AZIN1_HUMAN; OAZI; OAZIN; ODC1L; Ornithine decarboxylase antizyme inhibitor;
Immunogens
- O14977 AZIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQWQNVVAQIKPFYTVKCNSAPAVLEILAALGTGFACSSKNEMALVQELGVPPENIIYISPCKQVSQIKYAAKVGVNILTCDNEIELKKIARNHPNAKVLLHIATEDNIGGEEGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKESQVYVHALSDARCVFDMAGEIGFTMNMLDIGGGFTGTEFQLEEVNHVISPLLDIYFPEGSGVKIISEPGSYYVSSAFTLAVNIIAKKVVENDKFPSGVEKTGSDEPAFMYYMNDGVYGSFASKLSEDLNTIPEVHKKYKEDEPLFTSSLWGPSCDELDQIVESCLLPELNVGDWLIFDNMGADSFHEPSAFNDFQRPAIYYMMSFSDWYEMQDAGITSDSMMKNFFFVPSCIQLSQEDSFSAEA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14977 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K340 | Ubiquitination | Uniprot |
Research Backgrounds
Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.
Ubiquitinated, leading to its proteasomal degradation; a process that is reduced in presence of antizyme OAZ1.
Nucleus.
Expressed in liver.
Monomer. Interacts with OAZ1 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1 (Probable).
Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.
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