BBS10 Antibody | Affinity Biosciences

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IF/ICC

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BBS10 Antibody - Western blot analysis of extracts from 3T3, using BBS10 antibody.

BBS10 Antibody - DF12356 staining HUVEC cells by IF/ICC.

Product:	BBS10 Antibody
Catalog:	DF12356
Description:	Rabbit polyclonal antibody to BBS10
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Zebrafish
Prediction:	Pig, Horse, Sheep, Rabbit
Mol.Wt.:	81 kDa; 81kD(Calculated).
Uniprot:	Q8TAM1
RRID:	AB_2845161

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Zebrafish

Prediction:

Pig(91%), Horse(82%), Sheep(82%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

BBS10 Antibody detects endogenous levels of total BBS10.

RRID:

AB_2845161
Cite Format: Affinity Biosciences Cat# DF12356, RRID:AB_2845161.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1300007O09Rik; AI452285; Bardet Biedl syndrome 10 protein; Bardet Biedl syndrome 10 protein homolog; Bardet-Biedl syndrome 10; C12orf58; Chromosome 12 open reading frame 58; FLJ23560; RGD1560748;

Immunogens

Immunogen:

Uniprot:

Q8TAM1(BBS10_HUMAN) >>Visit HPA database.

Gene(ID):

BBS10(79738)

Sequence:

Q8TAM1 BBS10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSSMAAAGSVKAALQVAEVLEAIVSCCVGPEGRQVLCTKPTGEVLLSRNGGRLLEALHLEHPIARMIVDCVSSHLKKTGDGAKTFIIFLCHLLRGLHAITDREKDPLMCENIQTHGRHWKNCSRWKFISQALLTFQTQILDGIMDQYLSRHFLSIFSSAKERTLCRSSLELLLEAYFCGRVGRNNHKFISQLMCDYFFKCMTCKSGIGVFELVDDHFVELNVGVTGLPVSDSRIIAGLVLQKDFSVYRPADGDMRMVIVTETIQPLFSTSGSEFILNSEAQFQTSQFWIMEKTKAIMKHLHSQNVKLLISSVKQPDLVSYYAGVNGISVVECLSSEEVSLIRRIIGLSPFVPPQAFSQCEIPNTALVKFCKPLILRSKRYVHLGLISTCAFIPHSIVLCGPVHGLIEQHEDALHGALKMLRQLFKDLDLNYMTQTNDQNGTSSLFIYKNSGESYQAPDPGNGSIQRPYQDTVAENKDALEKTQTYLKVHSNLVIPDVELETYIPYSTPTLTPTDTFQTVETLTCLSLERNRLTDYYEPLLKNNSTAYSTRGNRIEISYENLQVTNITRKGSMLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Sheep

Dog

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TAM1 As Substrate

Q8TAM1

Site	PTM Type	Source
K40	Ubiquitination	Uniprot
T135	Phosphorylation	Uniprot
Y148	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
K161	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
K307	Ubiquitination	Uniprot
K372	Ubiquitination	Uniprot
S378	Phosphorylation	Uniprot
K477	Ubiquitination	Uniprot
K482	Ubiquitination	Uniprot
Y536	Phosphorylation	Uniprot
K542	Ubiquitination	Uniprot
K642	Acetylation	Uniprot
K646	Sumoylation	Uniprot
K713	Ubiquitination	Uniprot
S719	Phosphorylation	Uniprot

Research Backgrounds

Q8TAM1_BBS10_HUMAN

Function:

Probable molecular chaperone that assists the folding of proteins upon ATP hydrolysis. Plays a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.

Subcellular Location:

Cell projection>Cilium.
Note: Located within the basal body of the primary cilium of differentiating preadipocytes.

Subunit Structure:

Component of a complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8.

Family&Domains:

Belongs to the TCP-1 chaperonin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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BBS10 Antibody - #DF12356