BBS4 Antibody - #DF12358
| Product: | BBS4 Antibody |
| Catalog: | DF12358 |
| Description: | Rabbit polyclonal antibody to BBS4 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 50-60 kDa; 58kD(Calculated). |
| Uniprot: | Q96RK4 |
| RRID: | AB_2845163 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12358, RRID:AB_2845163.
Fold/Unfold
Bardet Biedl syndrome 4 protein; Bardet-Biedl syndrome 4 protein; Bbs4; BBS4_HUMAN;
Immunogens
- Q96RK4 BBS4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNLEDIENAKRAYAEAVHLDKCNPLVNLNYAVLLYNQGEKKNALAQYQEMEKKVSLLKDNSSLEFDSEMVEMAQKLGAALQVGEALVWTKPVKDPKSKHQTTSTSKPASFQQPLGSNQALGQAMSSAAAYRTLPSGAGGTSQFTKPPSLPLEPEPAVESSPTETSEQIREK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96RK4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K20 | Acetylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| Y478 | Phosphorylation | Uniprot |
Research Backgrounds
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell projection>Cilium membrane. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cell projection>Cilium>Flagellum. Cell projection>Cilium.
Note: Localizes to the pericentriolar material. Centrosomal localization requires dynein (By similarity). Localizes to the connecting cilium of photoreceptor cells (By similarity).
Ubiquitously expressed. The highest level of expression is found in the kidney.
Part of BBSome complex, that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts with PCM1 and DCTN1. Interacts with DC28B. Interacts with ALDOB and C2CD3. Interacts with PKD1. Interacts with CEP290.
Belongs to the BBS4 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.