ETFA Antibody - #DF12394

(3)  
Product: ETFA Antibody
Catalog: DF12394
Description: Rabbit polyclonal antibody to ETFA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 30 kDa; 35kD(Calculated).
Uniprot: P13804
RRID: AB_2845199

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Horse(92%), Rabbit(92%), Dog(89%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
ETFA Antibody detects endogenous levels of total ETFA.
RRID:
AB_2845199
Cite Format: Affinity Biosciences Cat# DF12394, RRID:AB_2845199.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha ETF; Alpha-ETF; Electron transfer flavoprotein alpha polypeptide; Electron transfer flavoprotein alpha subunit; Electron transfer flavoprotein subunit alpha mitochondrial; Electron transfer flavoprotein subunit alpha, mitochondrial; Electron transferring flavoprotein alpha polypeptide; EMA; ETFA; ETFA_HUMAN; GA2; Glutaric aciduria II;

Immunogens

Immunogen:
Uniprot:

P13804(ETFA_HUMAN) >>Visit HPA database.

Gene(ID):
ETFA(2108)
Sequence:
MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Horse
92
Pig
91
Dog
89
Chicken
83
Xenopus
75
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13804 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S32 Phosphorylation
T37 Phosphorylation
T42 Phosphorylation
T45 Phosphorylation
S52 Phosphorylation
C53 S-Nitrosylation
K62 Ubiquitination
K69 Acetylation
K69 Ubiquitination
K75 Ubiquitination
Y84 Phosphorylation
K85 Acetylation
K85 Ubiquitination
T93 Phosphorylation
K101 Acetylation
K126 Ubiquitination
K139 Acetylation
K139 Ubiquitination
S140 Phosphorylation
T143 Phosphorylation
Y149 Phosphorylation
K158 Ubiquitination
K164 Ubiquitination
R169 Methylation
T171 Phosphorylation
S172 Phosphorylation
S179 Phosphorylation
K187 Ubiquitination
S190 Phosphorylation
S192 Phosphorylation
K203 Acetylation
K203 Ubiquitination
K206 Ubiquitination
S207 Phosphorylation
T213 Phosphorylation
K216 Ubiquitination
R223 Methylation
K226 Ubiquitination
S227 Phosphorylation
Y235 Phosphorylation
S248 Phosphorylation
K294 Ubiquitination
K331 Ubiquitination

Research Backgrounds

Function:

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity.

Family&Domains:

Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.

Belongs to the ETF alpha-subunit/FixB family.

Restrictive clause

 

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For Research Use Only.
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