EXOC5 Antibody - #DF12397

(1)   (3)  
Product: EXOC5 Antibody
Catalog: DF12397
Description: Rabbit polyclonal antibody to EXOC5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 77 kDa; 82kD(Calculated).
Uniprot: O00471
RRID: AB_2845202

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
EXOC5 Antibody detects endogenous levels of total EXOC5.
RRID:
AB_2845202
Cite Format: Affinity Biosciences Cat# DF12397, RRID:AB_2845202.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI447711; AI448003; DKFZp666H126; EXOC5; exocyst complex component 5; Exocyst complex component Sec10; Gm76; HSEC10; p71 component of rsec6/8 secretory complex; PRO1912; SEC10; SEC10 like 1; SEC10L1; SEC10P;

Immunogens

Immunogen:
Uniprot:

O00471(EXOC5_HUMAN) >>Visit HPA database.

Gene(ID):
EXOC5(10640)
Expression:
O00471 EXOC5_HUMAN:

Ubiquitous.

Sequence:
MATTAELFEEPFVADEYIERLVWRTPGGGSRGGPEAFDPKRLLEEFVNHIQELQIMDERIQRKVEKLEQQCQKEAKEFAKKVQELQKSNQVAFQHFQELDEHISYVATKVCHLGDQLEGVNTPRQRAVEAQKLMKYFNEFLDGELKSDVFTNSEKIKEAADIIQKLHLIAQELPFDRFSEVKSKIASKYHDLECQLIQEFTSAQRRGEISRMREVAAVLLHFKGYSHCVDVYIKQCQEGAYLRNDIFEDAGILCQRVNKQVGDIFSNPETVLAKLIQNVFEIKLQSFVKEQLEECRKSDAEQYLKNLYDLYTRTTNLSSKLMEFNLGTDKQTFLSKLIKSIFISYLENYIEVETGYLKSRSAMILQRYYDSKNHQKRSIGTGGIQDLKERIRQRTNLPLGPSIDTHGETFLSQEVVVNLLQETKQAFERCHRLSDPSDLPRNAFRIFTILVEFLCIEHIDYALETGLAGIPSSDSRNANLYFLDVVQQANTIFHLFDKQFNDHLMPLISSSPKLSECLQKKKEIIEQMEMKLDTGIDRTLNCMIGQMKHILAAEQKKTDFKPEDENNVLIQYTNACVKVCAYVRKQVEKIKNSMDGKNVDTVLMELGVRFHRLIYEHLQQYSYSCMGGMLAICDVAEYRKCAKDFKIPMVLHLFDTLHALCNLLVVAPDNLKQVCSGEQLANLDKNILHSFVQLRADYRSARLARHFS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
92
Xenopus
73
Zebrafish
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00471 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
T4 Phosphorylation
K87 Ubiquitination
T122 Phosphorylation
K132 Ubiquitination
K135 Ubiquitination
K146 Ubiquitination
S147 Phosphorylation
K155 Ubiquitination
K157 Ubiquitination
K165 Ubiquitination
R177 Methylation
S210 Phosphorylation
Y225 Phosphorylation
K259 Ubiquitination
K289 Ubiquitination
K297 Ubiquitination
T328 Phosphorylation
T332 Phosphorylation
S378 Phosphorylation
K388 Ubiquitination
K557 Ubiquitination

Research Backgrounds

Function:

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

Subcellular Location:

Cytoplasm. Midbody.
Note: Localization at the midbody requires the presence of RALA, EXOC2 and EXOC3.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1 (By similarity).

Family&Domains:

Belongs to the SEC10 family.

References

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=6.5]

Application: IHC    Species: Human    Sample: LC tissue

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

Application: WB    Species: Human    Sample: HN4 and NP69 cells

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.