LAPTM4B Antibody - #DF12416
Product: | LAPTM4B Antibody |
Catalog: | DF12416 |
Description: | Rabbit polyclonal antibody to LAPTM4B |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Mol.Wt.: | 24 kDa, 37 kDa; 35kD(Calculated). |
Uniprot: | Q86VI4 |
RRID: | AB_2845221 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12416, RRID:AB_2845221.
Fold/Unfold
LAPTM4beta; LC27; Lysosomal associated protein transmembrane 4 beta; Lysosomal associated transmembrane protein 4 beta; Lysosomal associated transmembrane protein 4B; Lysosomal protein transmembrane 4 beta;
Immunogens
A synthesized peptide derived from human LAPTM4B, corresponding to a region within the internal amino acids.
- Q86VI4 LAP4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA
Research Backgrounds
Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.
Undergoes proteolytic cleavage following delivery to the lysosomes.
Ubiquitinated by NEDD4.
Endomembrane system>Multi-pass membrane protein. Late endosome membrane. Cell membrane. Cell projection. Lysosome membrane. Endosome membrane. Endosome>Multivesicular body membrane. Endosome>Multivesicular body lumen.
Belongs to the LAPTM4/LAPTM5 transporter family.
Research Fields
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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