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LETM1 Antibody - Western blot analysis of extracts from various samples, using LETM1 Antibody.

Product:	LETM1 Antibody
Catalog:	DF12417
Description:	Rabbit polyclonal antibody to LETM1
Application:	WB IHC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	83 kDa; 83kD(Calculated).
Uniprot:	O95202
RRID:	AB_2845222

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

LETM1 Antibody detects endogenous levels of total LETM1.

RRID:

AB_2845222
Cite Format: Affinity Biosciences Cat# DF12417, RRID:AB_2845222.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

LETM 1; LETM1 and EF hand domain containing protein 1, mitochondrial; LETM1 and EF-hand domain-containing protein 1; Letm1; LETM1_HUMAN; Leucine zipper EF hand containing transmembrane protein 1; Leucine zipper-EF-hand-containing transmembrane protein 1; Mdm38 homolog; mitochondrial;

Immunogens

Immunogen:

A synthesized peptide derived from human LETM1, corresponding to a region within the internal amino acids.

Uniprot:

O95202(LETM1_HUMAN) >>Visit HPA database.

Gene(ID):

LETM1(3954)

Sequence:

O95202 LETM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHLGCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGSATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O95202_LETM1_HUMAN

Function:

Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein.

Family&Domains:

Belongs to the LETM1 family.

References

1). LETM1 Promotes Gastric Cancer Cell Proliferation, Migration, and Invasion via the PI3K/Akt Signaling Pathway. Journal of Gastric Cancer, 2020 (PubMed: 32595998) [IF=3.2]

Application: WB Species: human Sample: GC cell lines and tissues

Fig. 1. |LETM1 is overexpressed in the human GC cell lines and tissues.(D) The protein expression levels of LETM1 in the GC cell lines (MGC-803, AGS, and MKN-45) and GES-1 cells. Data are presented as mean±standard deviation.LETM1 = leucine zipper-EF-hand containing transmembrane protein 1; GC = gastric cancer; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; HR = hazard ratio.*P<0.01.

Restrictive clause

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For Research Use Only.
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LETM1 Antibody - #DF12417