EIF2D Antibody | Affinity Biosciences

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IF/ICC

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EIF2D Antibody - Western blot analysis of extracts from HepG2, using LGTN Antibody.

EIF2D Antibody - DF12419 staining Hela by IF/ICC.

Product:	EIF2D Antibody
Catalog:	DF12419
Description:	Rabbit polyclonal antibody to EIF2D
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	65 kDa; 65kD(Calculated).
Uniprot:	P41214
RRID:	AB_2845224

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

EIF2D Antibody detects endogenous levels of total EIF2D.

RRID:

AB_2845224
Cite Format: Affinity Biosciences Cat# DF12419, RRID:AB_2845224.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Eukaryotic translation initiation factor 2D; EIF2D; HCA56; Hepatocellular carcinoma associated antigen 56; Hepatocellular carcinoma-associated antigen 56; LGTN; LIGA_HUMAN; Ligatin; OTTHUMP00000034537; OTTHUMP00000034538;

Immunogens

Immunogen:

Uniprot:

P41214(EIF2D_HUMAN) >>Visit HPA database.

Gene(ID):

EIF2D(1939)

Sequence:

P41214 EIF2D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDAVTVYVSGGNPILFELEKNLYPTVYTLWSYPDLLPTFTTWPLVLEKLVGGADLMLPGLVMPPAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDHLWRSGNKSSPPSIAPLALDSADLSEEKGSVQMDSTLQGDMRHMTLEGEEENGEVHQAREDKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVESIVAVDWKHPRITSFVIPEPSPTSQTIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPILCDCILEKNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHHLGWLLLEEYQLPRKHIQGLEKALKPGKKK

PTMs - P41214 As Substrate

P41214

Site	PTM Type	Source
M1	Acetylation	Uniprot
K4	Ubiquitination	Uniprot
S17	Phosphorylation	Uniprot
K45	Ubiquitination	Uniprot
K52	Ubiquitination	Uniprot
K182	Ubiquitination	Uniprot
S184	Phosphorylation	Uniprot
S195	Phosphorylation	Uniprot
S199	Phosphorylation	Uniprot
T219	Phosphorylation	Uniprot
S237	Phosphorylation	Uniprot
S239	Phosphorylation	Uniprot
K333	Acetylation	Uniprot
S361	Phosphorylation	Uniprot
T366	Phosphorylation	Uniprot
K402	Ubiquitination	Uniprot
S404	Phosphorylation	Uniprot
K430	Ubiquitination	Uniprot
K446	Ubiquitination	Uniprot
K454	Ubiquitination	Uniprot
S459	Phosphorylation	Uniprot
K467	Ubiquitination	Uniprot
K484	Ubiquitination	Uniprot
K569	Ubiquitination	Uniprot
K576	Ubiquitination	Uniprot

Research Backgrounds

P41214_EIF2D_HUMAN

Function:

Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.

Subcellular Location:

Cytoplasm.

Family&Domains:

Belongs to the eIF2D family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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EIF2D Antibody - #DF12419