MAP1B Antibody - #DF12422

(2)  
Product: MAP1B Antibody
Catalog: DF12422
Description: Rabbit polyclonal antibody to MAP1B
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 320 kDa; 271kD(Calculated).
Uniprot: P46821
RRID: AB_2845227

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
MAP1B Antibody detects endogenous levels of total MAP1B.
RRID:
AB_2845227
Cite Format: Affinity Biosciences Cat# DF12422, RRID:AB_2845227.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FUTSCH; LC1; MAP-1B; MAP1 light chain LC1; Map1b; MAP1B_HUMAN; MAP5; Microtubule associated protein 1B; Mtap1b; Mtap5;

Immunogens

Immunogen:
Uniprot:

P46821(MAP1B_HUMAN) >>Visit HPA database.

Gene(ID):
MAP1B(4131)
Sequence:
MATVVVEATEPEPSGSIANPAASTSPSLSHRFLDSKFYLLVVVGEIVTEEHLRRAIGNIELGIRSWDTNLIECNLDQELKLFVSRHSARFSPEVPGQKILHHRSDVLETVVLINPSDEAVSTEVRLMITDAARHKLLVLTGQCFENTGELILQSGSFSFQNFIEIFTDQEIGELLSTTHPANKASLTLFCPEEGDWKNSNLDRHNLQDFINIKLNSASILPEMEGLSEFTEYLSESVEVPSPFDILEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGFNMLINGGSERKSCFWKLIRHLDRVDSILLTHIGDDNLPGINSMLQRKIAELEEEQSQGSTTNSDWMKNLISPDLGVVFLNVPENLKNPEPNIKMKRSIEEACFTLQYLNKLSMKPEPLFRSVGNTIDPVILFQKMGVGKLEMYVLNPVKSSKEMQYFMQQWTGTNKDKAEFILPNGQEVDLPISYLTSVSSLIVWHPANPAEKIIRVLFPGNSTQYNILEGLEKLKHLDFLKQPLATQKDLTGQVPTPVVKQTKLKQRADSRESLKPAAKPLPSKSVRKESKEETPEVTKVNHVEKPPKVESKEKVMVKKDKPIKTETKPSVTEKEVPSKEEPSPVKAEVAEKQATDVKPKAAKEKTVKKETKVKPEDKKEEKEKPKKEVAKKEDKTPIKKEEKPKKEEVKKEVKKEIKKEEKKEPKKEVKKETPPKEVKKEVKKEEKKEVKKEEKEPKKEIKKLPKDAKKSSTPLSEAKKPAALKPKVPKKEESVKKDSVAAGKPKEKGKIKVIKKEGKAAEAVAAAVGTGATTAAVMAAAGIAAIGPAKELEAERSLMSSPEDLTKDFEELKAEEVDVTKDIKPQLELIEDEEKLKETEPVEAYVIQKEREVTKGPAESPDEGITTTEGEGECEQTPEELEPVEKQGVDDIEKFEDEGAGFEESSETGDYEEKAETEEAEEPEEDGEEHVCVSASKHSPTEDEESAKAEADAYIREKRESVASGDDRAEEDMDEAIEKGEAEQSEEEADEEDKAEDAREEEYEPEKMEAEDYVMAVVDKAAEAGGAEEQYGFLTTPTKQLGAQSPGREPASSIHDETLPGGSESEATASDEENREDQPEEFTATSGYTQSTIEISSEPTPMDEMSTPRDVMSDETNNEETESPSQEFVNITKYESSLYSQEYSKPADVTPLNGFSEGSKTDATDGKDYNASASTISPPSSMEEDKFSRSALRDAYCSEVKASTTLDIKDSISAVSSEKVSPSKSPSLSPSPPSPLEKTPLGERSVNFSLTPNEIKVSAEAEVAPVSPEVTQEVVEEHCASPEDKTLEVVSPSQSVTGSAGHTPYYQSPTDEKSSHLPTEVIEKPPAVPVSFEFSDAKDENERASVSPMDEPVPDSESPIEKVLSPLRSPPLIGSESAYESFLSADDKASGRGAESPFEEKSGKQGSPDQVSPVSEMTSTSLYQDKQEGKSTDFAPIKEDFGQEKKTDDVEAMSSQPALALDERKLGDVSPTQIDVSQFGSFKEDTKMSISEGTVSDKSATPVDEGVAEDTYSHMEGVASVSTASVATSSFPEPTTDDVSPSLHAEVGSPHSTEVDDSLSVSVVQTPTTFQETEMSPSKEECPRPMSISPPDFSPKTAKSRTPVQDHRSEQSSMSIEFGQESPEQSLAMDFSRQSPDHPTVGAGVLHITENGPTEVDYSPSDMQDSSLSHKIPPMEEPSYTQDNDLSELISVSQVEASPSTSSAHTPSQIASPLQEDTLSDVAPPRDMSLYASLTSEKVQSLEGEKLSPKSDISPLTPRESSPLYSPTFSDSTSAVKEKTATCHSSSSPPIDAASAEPYGFRASVLFDTMQHHLALNRDLSTPGLEKDSGGKTPGDFSYAYQKPEETTRSPDEEDYDYESYEKTTRTSDVGGYYYEKIERTTKSPSDSGYSYETIGKTTKTPEDGDYSYEIIEKTTRTPEEGGYSYDISEKTTSPPEVSGYSYEKTERSRRLLDDISNGYDDSEDGGHTLGDPSYSYETTEKITSFPESEGYSYETSTKTTRTPDTSTYCYETAEKITRTPQASTYSYETSDLCYTAEKKSPSEARQDVDLCLVSSCEYKHPKTELSPSFINPNPLEWFASEEPTEESEKPLTQSGGAPPPPGGKQQGRQCDETPPTSVSESAPSQTDSDVPPETEECPSITADANIDSEDESETIPTDKTVTYKHMDPPPAPVQDRSPSPRHPDVSMVDPEALAIEQNLGKALKKDLKEKTKTKKPGTKTKSSSPVKKSDGKSKPLAASPKPAGLKESSDKVSRVASPKKKESVEKAAKPTTTPEVKAARGEEKDKETKNAANASASKSAKTATAGPGTTKTTKSSAVPPGLPVYLDLCYIPNHSNSKNVDVEFFKRVRSSYYVVSGNDPAAEEPSRAVLDALLEGKAQWGSNMQVTLIPTHDSEVMREWYQETHEKQQDLNIMVLASSSTVVMQDESFPACKIEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
92
Xenopus
83
Horse
0
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46821 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S14 Phosphorylation
S25 Phosphorylation
S91 Phosphorylation
T129 Phosphorylation
R203 Methylation
K259 Ubiquitination
S336 Phosphorylation
S339 Phosphorylation
T340 Phosphorylation
S343 Phosphorylation
S351 Phosphorylation
T405 Phosphorylation
Y423 Phosphorylation
K506 Acetylation
T517 Phosphorylation
T527 Phosphorylation
S541 Phosphorylation
S544 Phosphorylation
K546 Ubiquitination
S561 Phosphorylation
T565 Phosphorylation
T569 Phosphorylation
S582 Phosphorylation
S601 Phosphorylation
S609 Phosphorylation
S614 Phosphorylation
K636 Acetylation
K643 Acetylation
T667 Phosphorylation
K685 Acetylation
K686 Acetylation
K693 Acetylation
K694 Acetylation
T704 Phosphorylation
K707 Acetylation
K714 Acetylation
K715 Acetylation
K719 Acetylation
T744 Phosphorylation
S747 Phosphorylation
K790 Acetylation
S828 Phosphorylation
S831 Phosphorylation
S832 Phosphorylation
T837 Phosphorylation
Y876 Phosphorylation
S891 Phosphorylation
T897 Phosphorylation
C905 S-Nitrosylation
T908 Phosphorylation
S936 Phosphorylation
S937 Phosphorylation
T939 Phosphorylation
T948 Phosphorylation
S970 Phosphorylation
T972 Phosphorylation
S977 Phosphorylation
Y985 Phosphorylation
S992 Phosphorylation
S995 Phosphorylation
R999 Methylation
S1016 Phosphorylation
Y1044 Phosphorylation
Y1062 Phosphorylation
T1066 Phosphorylation
T1067 Phosphorylation
T1069 Phosphorylation
S1076 Phosphorylation
S1144 Phosphorylation
T1147 Phosphorylation
T1152 Phosphorylation
S1154 Phosphorylation
S1156 Phosphorylation
T1163 Phosphorylation
Y1170 Phosphorylation
S1171 Phosphorylation
Y1174 Phosphorylation
S1187 Phosphorylation
Y1200 Phosphorylation
S1203 Phosphorylation
T1206 Phosphorylation
S1208 Phosphorylation
S1211 Phosphorylation
S1212 Phosphorylation
S1221 Phosphorylation
Y1227 Phosphorylation
S1229 Phosphorylation
S1247 Phosphorylation
S1248 Phosphorylation
S1252 Phosphorylation
S1254 Phosphorylation
S1256 Phosphorylation
S1258 Phosphorylation
S1260 Phosphorylation
S1262 Phosphorylation
S1265 Phosphorylation
T1270 Phosphorylation
S1276 Phosphorylation
S1280 Phosphorylation
T1282 Phosphorylation
S1298 Phosphorylation
T1302 Phosphorylation
S1312 Phosphorylation
T1317 Phosphorylation
S1322 Phosphorylation
S1324 Phosphorylation
S1326 Phosphorylation
T1328 Phosphorylation
S1330 Phosphorylation
T1334 Phosphorylation
Y1336 Phosphorylation
Y1337 Phosphorylation
S1339 Phosphorylation
T1341 Phosphorylation
S1346 Phosphorylation
S1362 Phosphorylation
S1376 Phosphorylation
S1378 Phosphorylation
S1387 Phosphorylation
S1389 Phosphorylation
S1396 Phosphorylation
S1400 Phosphorylation Q13627 (DYRK1A)
S1406 Phosphorylation
S1408 Phosphorylation
Y1410 Phosphorylation
S1412 Phosphorylation
S1415 Phosphorylation
S1421 Phosphorylation
S1427 Phosphorylation
S1433 Phosphorylation
S1438 Phosphorylation
S1443 Phosphorylation
S1446 Phosphorylation
T1449 Phosphorylation
S1450 Phosphorylation
T1451 Phosphorylation
S1452 Phosphorylation
Y1454 Phosphorylation
S1462 Phosphorylation
S1501 Phosphorylation
T1503 Phosphorylation
S1508 Phosphorylation
S1512 Phosphorylation
T1517 Phosphorylation
S1520 Phosphorylation
T1525 Phosphorylation
S1527 Phosphorylation
Y1543 Phosphorylation
S1618 Phosphorylation
S1620 Phosphorylation
S1625 Phosphorylation
T1628 Phosphorylation
S1631 Phosphorylation
T1633 Phosphorylation
S1646 Phosphorylation
S1653 Phosphorylation
S1657 Phosphorylation
S1666 Phosphorylation
T1671 Phosphorylation
T1680 Phosphorylation
T1685 Phosphorylation
Y1689 Phosphorylation
S1690 Phosphorylation
S1743 Phosphorylation
S1760 Phosphorylation
Y1762 Phosphorylation
S1764 Phosphorylation
S1772 Phosphorylation
S1779 Phosphorylation
S1782 Phosphorylation
S1785 Phosphorylation P28482 (MAPK1)
T1788 Phosphorylation
S1792 Phosphorylation
S1793 Phosphorylation
Y1796 Phosphorylation
S1797 Phosphorylation
T1799 Phosphorylation
S1801 Phosphorylation
T1804 Phosphorylation
S1805 Phosphorylation
K1810 Ubiquitination
T1811 Phosphorylation
T1813 Phosphorylation
C1814 S-Nitrosylation
S1816 Phosphorylation
S1817 Phosphorylation
S1818 Phosphorylation
S1819 Phosphorylation
S1826 Phosphorylation
Y1830 Phosphorylation
T1840 Phosphorylation
S1852 Phosphorylation
T1853 Phosphorylation
K1858 Ubiquitination
K1863 Ubiquitination
T1864 Phosphorylation
S1869 Phosphorylation
Y1870 Phosphorylation
Y1872 Phosphorylation
K1874 Ubiquitination
T1878 Phosphorylation
T1879 Phosphorylation
R1880 Methylation
S1881 Phosphorylation
Y1887 Phosphorylation
Y1889 Phosphorylation
S1891 Phosphorylation
Y1892 Phosphorylation
K1894 Ubiquitination
T1898 Phosphorylation
S1899 Phosphorylation
Y1904 Phosphorylation
Y1905 Phosphorylation
Y1906 Phosphorylation
K1908 Ubiquitination
T1912 Phosphorylation
T1913 Phosphorylation
K1914 Ubiquitination
S1915 Phosphorylation
S1917 Phosphorylation
S1919 Phosphorylation
Y1921 Phosphorylation
S1922 Phosphorylation
Y1923 Phosphorylation
T1925 Phosphorylation
K1928 Ubiquitination
T1930 Phosphorylation
K1931 Ubiquitination
T1932 Phosphorylation
Y1938 Phosphorylation
S1939 Phosphorylation
Y1940 Phosphorylation
K1945 Ubiquitination
T1946 Phosphorylation
T1947 Phosphorylation
T1949 Phosphorylation
Y1955 Phosphorylation
S1956 Phosphorylation
Y1957 Phosphorylation
S1960 Phosphorylation
T1963 Phosphorylation
T1964 Phosphorylation
S1965 Phosphorylation
S1970 Phosphorylation
Y1972 Phosphorylation
S1973 Phosphorylation
Y1974 Phosphorylation
K1976 Ubiquitination
S1988 Phosphorylation
Y1991 Phosphorylation
S1994 Phosphorylation
S2005 Phosphorylation
Y2006 Phosphorylation
Y2008 Phosphorylation
Y2023 Phosphorylation
S2024 Phosphorylation
Y2025 Phosphorylation
K2030 Ubiquitination
T2031 Phosphorylation
T2034 Phosphorylation
T2037 Phosphorylation
S2038 Phosphorylation
Y2040 Phosphorylation
C2041 S-Nitrosylation
Y2042 Phosphorylation
K2047 Ubiquitination
T2051 Phosphorylation
S2055 Phosphorylation
Y2059 Phosphorylation
S2062 Phosphorylation
T2067 Phosphorylation
S2072 Phosphorylation
S2074 Phosphorylation
S2086 Phosphorylation
Y2090 Phosphorylation
T2095 Phosphorylation
S2098 Phosphorylation
S2100 Phosphorylation
S2180 Phosphorylation
T2192 Phosphorylation
T2194 Phosphorylation
Y2195 Phosphorylation
R2208 Methylation
S2209 Phosphorylation
S2211 Phosphorylation
S2218 Phosphorylation
K2237 Acetylation
K2242 Acetylation
K2246 Acetylation
K2247 Acetylation
K2253 Acetylation
S2256 Phosphorylation
K2259 Acetylation
S2265 Phosphorylation
S2271 Phosphorylation
S2289 Phosphorylation
T2303 Phosphorylation
T2305 Phosphorylation
T2334 Phosphorylation
Y2433 Phosphorylation
S2450 Phosphorylation

Research Backgrounds

Function:

Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.

PTMs:

LC1 is generated from MAP1B by proteolytic processing.

S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm. Cell junction>Synapse. Cell projection>Dendritic spine.
Note: Colocalizes with DAPK1 in the microtubules and cortical actin fibers.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. LC1 interacts with the amino-terminal region of MAP1B. Interacts with ANP32A and TIAM2. Interacts with the tubulin tyrosine TTL (By similarity). Interacts (via C-terminus) with GAN (via Kelch domains). Interacts (via N-terminus) with DAPK1. Interacts with TMEM185A. Interacts with MAP1LC3B. Interacts with KIRREL3. MAP1 light chain LC1 (via C-terminus): Interacts with ELAVL4; the interaction contributes to the association of ELAVL4 with microtubules (By similarity). MAP1 light chain LC1: Interacts with ELAVL2 and ELAVL3 (By similarity).

Family&Domains:

Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.

Belongs to the MAP1 family.

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